Anti-MICA/B antibodies that block MICA/B shedding and methods of use
Provided herein are antibodies that specifically bind to MICA/B having heavy chain, light chain, variable heavy chain domains (VH), variable light chain domains (VL), and complementarity determining regions (CDRs) disclosed herein, as well as methods and uses thereof.
1 . A monoclonal antibody or an antigen-binding fragment thereof, wherein the monoclonal antibody or antigen binding fragment thereof specifically binds to a MICA protein, a MICB protein, or both MICA and MICB, comprising
a. A light chain variable domain (VL) comprising an amino acid sequence 100% identical to SEQ ID NO: 7 and a heavy chain variable domain (VH) comprising an amino acid sequence 100% identical to SEQ ID NO: 8; or
b. A VL comprising an amino acid sequence 100% identical to SEQ ID NO: 15 and a heavy chain variable domain (VH) comprising an amino acid sequence 100% identical to SEQ ID NO: 16.
2 . The monoclonal antibody of claim 1 , wherein the monoclonal antibody or antigen binding fragment thereof is selected from a whole immunoglobulin, an scFv, a Fab, a F(ab′)2, or a disulfide linked Fv.
3 . The monoclonal antibody of claim 1 , wherein the monoclonal antibody or antigen binding fragment thereof is an IgG or IgM.
4 . The monoclonal antibody of claim 1 , wherein the monoclonal antibody or antigen binding fragment thereof is humanized or chimeric.