Recombinant antibody having unique glycan profile produced by CHO host cell with edited genome and preparation method thereof
The present invention, in the field of bioengineering and biotechnology, relates to a method for preparing a recombinant antibody with a unique glycan profile produced by a genome-edited CHO host cell. Specifically, according to a method of the present invention, the TALEN technology is used to edit the FUT8 gene in CHO cells that have been adapted for serum-free suspension growth. The edited CHO host cells can produce recombinant antibodies with a unique glycan profile. The unique glycan profile can be characterized by non-fucosylated N-linked oligosaccharide chains of the antibodies, extremely low N-glycosylation heterogeneity and uniform carbohydrate chains. The antibody prepared by the method of the invention exhibit significantly increased ADCC and greater stability.
1 . An antibody having specificity to CD20 and comprising two light chains each comprising the amino acid sequence of SEQ ID NO: 22 and two heavy chains each comprising the amino acid sequence of SEQ ID NO: 23, wherein the antibody contains no fucose and has a G0 content greater than or equal to 60% and less than or equal to 80%.
2 . The antibody of claim 1 , which has a mannose content that is less than or equal to 5%.
3 . A pharmaceutical composition, comprising the antibody of claim 1 and a pharmaceutically acceptable carrier.