Method for producing cell population containing CAR-expressing immune cells
The present disclosure includes a method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are normal blood cells that have been engineered to express the target antigen.
1 . A method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are cells that have been prepared by gene transfer into peripheral blood mononuclear cells (PBMCs), wherein the target antigen is Human epidermal growth factor receptor 2 (HER2) or Ephrin type-B receptor 4 (EPHB4).
2 . The method according to claim 1 , wherein the immune cells are lymphocytes.
3 . The method according to claim 1 , wherein the immune cells are T cells.
4 . The method according to claim 1 , wherein the CAR-expressing immune cells are cells that have been prepared by gene transfer into peripheral blood mononuclear cells (PBMCs).
5 . The method according to claim 1 , further comprising preparing the CAR-expressing immune cells.
6 . The method according to claim 5 , wherein the CAR-expressing immune cells are prepared by gene transfer into PBMCs.
7 . The method according to claim 5 , wherein the CAR-expressing immune cells are prepared by the piggyflac transposon-mediated method.
8 . The method according to claim 1 , wherein the target antigen-expressing cells are cells into which one or more genes of one or more co-stimulatory molecules have been introduced.
9 . The method according to claim 1 , wherein the gene transfer includes transfer of a target antigen gene.
10 . The method according to claim 1 , further comprising preparing the target antigen-expressing cells.
11 . The method according to claim 10 , wherein the target antigen-expressing cells are prepared from PBMCs.
12 . The method according to claim 11 , wherein the target antigen-expressing cells are prepared by gene transfer into PBMCs.
13 . The method according to claim 12 , wherein the gene transfer includes transfer of a target antigen gene.
14 . The method according to claim 8 , wherein the one or more co-stimulatory molecules are selected from CD40, CD80, 4-1BBL, and OX40L.
15 . A cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells produced by the method according to claim 1 .
16 . A method for treating cancer, comprising administering the cell population according to claim 15 to a subject.
17 . The method according to claim 16 , wherein the cancer is a solid tumor.