Blue-excitable water-solvated polymeric dyes
Water solvated polymeric dyes and polymeric tandem dyes having a blue excitation spectrum are provided. The polymeric dyes are conjugated polymers that can include a thiophene-containing co-monomer. The polymeric tandem dyes further include a signaling chromophore covalently linked to the conjugated polymer in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
1 . A kit comprising: a polymeric dye comprising a thiophene-containing co-monomer and a fused tricyclic co-monomer and having a blue excitation spectrum; and a container.
2 . The kit according to claim 1 , further comprising one or more components selected from the group consisting of a polymeric tandem dye, a fluorophore, a specific binding member, a specific binding member conjugate, a cell, a support, a biocompatible aqueous elution buffer and instructions for use.
3 . The kit according to claim 1 , wherein the polymeric dye is covalently linked to a specific binding member.
4 . The kit according to claim 3 , wherein the specific binding member is an antibody.
5 . The kit according to claim 3 , wherein the specific binding member is an antibody fragment or binding derivative thereof.
6 . The kit according to claim 5 , wherein the antibody fragment or binding derivative thereof is selected from the group consisting of a Fab fragment, a F(ab′)2 fragment, a scFv, a diabody and a triabody.
7 . The kit according to claim 1 , wherein polymeric dye further comprises an acceptor signaling chromophore covalently linked to the polymeric due in energy-receiving proximity therewith.
8 . The kit according to claim 1 , wherein the fused tricyclic co-monomer is described by the formula:
wherein:
Y is C(R 3 ) 2 , —C(R 3 ) 2 C(R 3 ) 2 —, NR 3 , Si(R 3 ) 2 or Se;
each Z is CH, CR or N;
each R 3 is independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, acyl, substituted acyl, alkoxy, substituted alkoxy, amido, substituted amido, an aralkyl, a substituted aralkyl, a PEG moiety, -L 1 -Z 1 , where L 1 is a linker and Z 1 is a chemoslective tag (e.g., a tag including a chemoslective functional group) and a WSG;
each R is independently H or one or more aryl or heteroaryl substituents, In some cases, each R refers to one or two ring substituents independently selected from halogen, sulfonate, alkoxy, substituted alkoxy, alkyl and substituted alkyl, In certain instances, at least two of Z in each ring is CH or CR, In certain instances, one and only one of Z in each ring is N; and
* denotes a site for covalent attachment to another co-monomer of the polymeric dye.