Disposable cell culture bag
Disclosed is a disposable cell culture bag capable of facilitating cell culture by using a flexible transparent resin film, the disposable cell culture bag including a body having a sealed space for accommodating a medium, and made of a flexible transparent resin film usable for disposable products, and one or more tubing lines extending from an inside to an outside of the body, wherein the body includes a bottom-side integrated part provided by extending one or more side surfaces from a bottom surface of the body to be spread in a radial shape, in order to obtain continuously smooth four edges of the bottom surface, and a top surface fusion bonded to the side surfaces of the bottom-side integrated part after the side surfaces are fusion bonded to each other.
1 . A method of fabricating a disposable cell culture bag, comprising:
folding up side surfaces of a bottom-side integrated part having a one-piece bottom surface and four rectangular side surfaces extending from the bottom surface, the bottom surface having a round shape and a unitary construction and being integrally formed with the one four rectangular side surfaces prior to bonding first bonding lines to one another, the first bonding lines being located at boundaries of the bottom-side integrated part, wherein the bottom-side integrated part is provided in a cross shape formed by the four rectangular side surfaces extending from the bottom surface without touching each other in combination with the bottom surface having the round shape;
fusion bonding the first bonding lines to one another;
fusion bonding a top surface to the four rectangular side surfaces of the bottom-side integrated part to thereby define a body having a sealed space for accommodating a medium and being made of a flexible transparent resin film used for disposable products; and
arranging one or more tubing lines so that the tubing lines extend from outside of the body, through the top surface to an inside of the body.
2 . The method of claim 1 , wherein the top surface has a rectangular shape corresponding to the side surfaces of the bottom-side integrated part, and comprises second fusion bonding lines at boundaries.
3 . The method of claim 1 , wherein each of the one or more tubing lines comprises a tube and flange, and further comprising:
fusion bonding the flange to an internal surface of the body; and
penetrating the tube through the body and fixing the tube to the body by arranging the tube in the flange so that the flange protrudes in an outer circumferential direction from the tube.
4 . The method of claim 1 , wherein the tubing lines comprise one or more of a temperature sensor line, a sampling line, a feeding line, a gas-in line, a gas-out line, an air line, a base line, or a combination thereof.
5 . The method of claim 4 , further comprising sealing the one or more tubing lines with fasteners to prevent external air from entering while air is filled in the body.
6 . The method of claim 1 , wherein the one or more tubing lines extend along the four rectangular side surfaces of the body to the bottom surface to minimize bubbles formed when a gas or a liquid is supplied from outside to the medium.
7 . The method of claim 1 , further comprising providing in the body a patch for measuring an acid level and a patch for measuring an oxygen saturation level.
8 . The method of claim 1 , wherein fusion bonding the first bonding lines comprises providing fusion bonded wings on an outside of the body.