Biological reaction apparatus and method for performing biological detection on basis of apparatus
A biological reaction apparatus used for biological macromolecules includes a power supply module ( 1 ), a control module ( 2 ), a liquid processing module ( 3 ), a reactor module ( 4 ) and a sensor ( 5 ); the power supply module ( 1 ) includes a direct current power supply ( 6 ) and a switch ( 7 ); the control module ( 2 ) includes a system controller ( 8 ), an input device ( 9 ) and an output device ( 10 ); the liquid processing module ( 3 ) includes a valve ( 11 ) or a combination of valves ( 11 ), a pump ( 12 ) or a combination of pumps ( 12 ) and sample cells ( 13 ); the reactor module ( 4 ) includes a reactor ( 14 ); the reactor ( 14 ) includes a reactor frame ( 15 ) and a reactor cavity ( 16 ) formed by the reactor frame ( 15 ). The apparatus has a simple and practical structure and is easy to operate. In the reactor ( 14 ), a very small amount of a reagent is used to achieve a uniform and highly-sensitive reaction.
1 . A biological reaction apparatus, comprising a power supply module, a control module, a liquid processing module, a reactor module and a sensor; wherein
the power supply module comprises a direct current power supply and a switch;
the control module comprises a system controller, an input device and an output device;
the liquid processing module comprises a valve or a combination of valves, a pump or a combination of pumps and sample cells;
the reactor module comprises a reactor;
the power supply module is separately connected to the control module, the liquid processing module and the reactor module through cables;
the control module is separately connected to the liquid processing module and the reactor module through the cables;
the liquid processing module is connected to the reactor module through a pipeline and configured to input a reactant to the reactor; and
the control module is connected to the reactor module through the sensor, wherein the reactor comprises a reactor frame, a pressing part, a sealing part, and a rotating shaft,
the reactor frame includes a reactor front plate, a reactor rear plate, and a limiting part, and a reactor cavity is formed between the reactor front plate and the reactor rear plate,
the limiting part is arranged in the reactor cavity between the reactor front plate and the reactor rear plate and configured to maintain the reactor cavity after the reactor is closed,
the pressing part is configured to press the reactor front plate and the reactor rear plate after the reactor is closed, and the pressing part is fixed to the reactor frame through the rotating shaft and is rotatable around the rotating shaft,
the sealing part is arranged between the reactor front plate and the reactor rear plate to seal the reactor cavity,
at least one opening is formed on the reactor frame, and a liquid flow or a gas flow enters the reactor cavity through the at least one opening and flows through a gap between the reactant and the reactor cavity to make the reactant suspended in the reactor cavity; and
the biological reaction apparatus further comprises a waste liquid collector configured to receive waste liquid from the reactor, a liquid level indicator, an air inlet pipeline, and a gas-liquid selection valve, wherein:
the liquid level indicator is maintained below a liquid level of the waste liquid collector,
the air inlet pipeline is maintained above the liquid level of the waste liquid collector to ensure that waste liquid is not sucked into the pipeline when the gas flow is introduced, and
the gas-liquid selection valve is connected to a tail end of the air inlet pipeline to ensure that gas intake and liquid discharge are isolated.
2 . The biological reaction apparatus according to claim 1 , wherein a mother liquid pool is also arranged between the liquid processing module and the reactor module.
3 . The biological reaction apparatus according to claim 1 , wherein an inner wall of the reactor is subjected to hydrophobization treatment or hydrophilization treatment; wherein the hydrophobization treatment is one or more of siliconization and alkylation; and wherein the hydrophilization treatment is one or more of hydroxylation, carboxylation and amination.
4 . The biological reaction apparatus according to claim 1 , wherein the reactor is made from a metal material or a polymer material, the metal material is selected from stainless steel and aluminum alloy, and the polymer material is selected from polypropylene and an acrylonitrile-styrene-butadiene copolymer.
5 . The biological reaction apparatus according to claim 1 , wherein the sealing part is made from an elastic material or a material repellent to a reaction solution in the biological reaction apparatus.
6 . The biological reaction apparatus according to claim 1 , wherein the reactor module further comprises an identification module, wherein the identification module is RFID.
7 . The biological reaction apparatus according to claim 1 , wherein the liquid processing module comprises one or more sample storage containers, and the sample storage containers are the sample cells.
8 . The biological reaction apparatus according to claim 1 , wherein the liquid processing module comprises at least one one-way and/or two-way pump, wherein the pump is one or more of a peristaltic pump, a diaphragm pump, a gear pump and a plunger pump.
9 . The biological reaction apparatus according to claim 1 , wherein the valve is one or more of a diaphragm valve, a pinch valve and a column valve.
10 . The biological reaction apparatus according to claim 1 , wherein the reactor is connected to the liquid processing module through the pipeline, a one-way valve or a two-way fast connector.
11 . The biological reaction apparatus according to claim 1 , wherein a liquid film or a gas film is formed between the reactant and a wall of the reactor to prevent a wall attachment effect of the reactant.
12 . The biological reaction apparatus according to claim 1 , wherein the at least one opening includes two liquid inlets arranged on the reactor front plate and the reactor rear plate, respectively.
13 . The biological reaction apparatus according to claim 1 , wherein the pressing part includes a front pressing structure arranged on the reactor front plate and a rear pressing structure arranged on the reactor rear plate, each of the front pressing structure and the rear pressing structure is fixed through the rotating shaft and is rotatable around the rotating shaft.
14 . The biological reaction apparatus according to claim 1 , further comprising at least one diversion block arranged within the reactor cavity to make the liquid flow entering the reactor cavity uniformly distributed.
15 . The biological reaction apparatus according to claim 1 , further comprising a two-way fast card set configured to connect the pipeline and the reactor, the two-way fast card set including a first fast card and a second fast card, wherein:
the first fast card comprises a first pagoda connector, a closure member, a first main body, a conductor, a first guide structure, an inner limiting structure, an outer limiting structure, and a first one-way valve function,
the first pagoda connector is connected to one of the pipeline or the reactor, and includes a first internal flow channel,
the closure member and the first main body form a containing cavity,
the conductor includes a second internal flow channel,
the inner limiting structure limits a backward distance of the conductor, and the outer limiting structure limits an outward distance of the conductor,
the second fast card comprises a second main body, a second pagoda connector, a second guide structure, and a second one-way valve structure,
the second pagoda connector is connected to the other one of the reactor and the pipeline, and includes a third internal flow channel,
the first guide structure and the second guide structure are configured for internal guide fixation,
the first one-way valve function and the second one-way valve structure are configured to control the first fast card to separate from the second fast card,
when the first fast card and the second fast card are connected, the third internal flow channel, the second internal flow channel, and the first internal flow channel are in communication.