Nucleic acid purification method, nucleic acid extraction liquid, and nucleic acid purification kit
Provided is a nucleic acid purification method capable of efficiently purifying a nucleic acid. The nucleic acid purification method including the steps of: mixing a nucleic acid with an antifoaming agent and a coprecipitation agent; and purifying the nucleic acid, in which the antifoaming agent is at least one of a nonionic surfactant and a silicone antifoaming agent.
1 . A nucleic acid purification method, comprising:
a step of mixing a sample containing a nucleic acid with an antifoaming agent and a coprecipitation agent, thus obtaining a nucleic acid extraction liquid containing the nucleic acid; and
a step of purifying the nucleic acid,
wherein
the antifoaming agent is at least one of a nonionic surfactant and a silicone antifoaming agent,
a content of the antifoaming agent in the nucleic acid extraction liquid containing the nucleic acid is 0.001 vol % or more and 1 vol % or less, and
the coprecipitation agent is a polyadenine.
2 . The nucleic acid purification method according to claim 1 , wherein the nucleic acid is mixed with the antifoaming agent and the coprecipitation agent, and then the nucleic acid is adsorbed on a solid phase carrier and purified.
3 . The nucleic acid purification method according to claim 1 , further comprising:
a step of preparing a nucleic acid extraction liquid containing the antifoaming agent and the coprecipitation agent in advance, wherein
the nucleic acid is mixed with the antifoaming agent and the coprecipitation agent by bringing a sample containing the nucleic acid into contact with the nucleic acid extraction liquid.
4 . The nucleic acid purification method according to claim 1 , wherein the antifoaming agent is the nonionic surfactant.
5 . The nucleic acid purification method according to claim 4 , wherein the nonionic surfactant is at least one selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyethylene branched alkyl ether, a polyoxyethylene octylphenyl ether, a polyoxyethylene distyrenated phenyl ether, a polyoxyethylene phytosteryl ether, a polyoxyalkylene fatty acid ester, a polyoxyalkylene glyceryl ether, a sorbitan fatty acid ester, a lauryl-β-D-maltoside, a polyoxyethylene alkylamine, a palm kernel oil fatty acid diethanolamide, and a digitonin.
6 . The nucleic acid purification method according to claim 4 , wherein the nonionic surfactant is a mixture of a polyoxyalkylene alkyl ether and a polyoxyalkylene fatty acid ester.
7 . The nucleic acid purification method according to claim 1 , wherein the antifoaming agent is the silicone antifoaming agent.
8 . The nucleic acid purification method according to claim 7 , wherein the silicone antifoaming agent includes a silicone oil and an emulsifier.
9 . The nucleic acid purification method according to claim 7 , wherein the silicone antifoaming agent is obtained by emulsifying the silicone oil with the nonionic surfactant.