Gene transfection system and method
The gene transfection system includes an acoustothermal module and a signal generating module; the acoustothermal module includes a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate and N sound-absorbing vessels arranged on the acoustothermal chip and used for cultivating recipient cells, and N is an integer greater than or equal to 1; the signal generating module is used to output basic frequency signals; the acoustothermal chip is used to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field with the acoustic wave signal, and control the temperature of the recipient cell in the sound-absorbing vessels with the temperature gradient field, so that the pores are opened on the membranes of the recipient cell, and the nucleic acids or other substances can go into the cells through these pores.
1 . A gene transfection system, wherein the system comprises: an acoustothermal module and a signal generating module;
the acoustothermal module comprises: a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate, and N sound-absorbing vessels arranged on the acoustothermal chip and configured for culturing recipient cells, wherein N is an integer greater than 1;
the signal generating module is configured to output a basic frequency signal;
the acoustothermal chip is configured to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field in the sound-absorbing vessels with the acoustic wave signal, and precisely control the temperature of the recipient cells in the sound-absorbing vessels to a specified temperature that enables a perforation effect on membranes of the recipient cells to reach a level of 100% by controlling an intensity of the acoustic wave signal at a predetermined acoustic wave signal intensity based on the temperature gradient field, so that pores are opened on the membranes of the recipient cells, and nucleic acids or other substances are allowed to go into the recipient cells through these pores;
wherein the temperature gradient field is a set of temperature values with gradient changes established based on changes of the acoustic wave signal, each temperature value corresponds to an acoustic wave signal strength and also corresponds to a perforation effect on the membranes of the recipient cells in a sound-absorbing vessel;
wherein the acoustothermal chip comprises M interdigital transducers, a first controller, and a second controller; and M is an integer greater than 1;
the sound-absorbing vessels are made of polydimethylsiloxane (PDMS), and each sound-absorbing vessel contains an independent chamber;
the interdigital transducer is used to generate a surface acoustic wave based on the basic frequency signal;
the first controller is used to individually control the finger period in each of the interdigital transducers;
the second controller is used to periodically activate part or all of the interdigital transducers;
the first controller and the second controller are further used to construct an array of the interdigital transducers to change the thermal field distribution in the acoustothermal chip.
2 . The gene transfection system of claim 1 , wherein the thermal field distribution in the acoustothermal chip is changed based on shapes of electrodes of the interdigital transducer.
3 . The gene transfection system of claim 1 , wherein the second controller is configured to simultaneously activate a group of interdigital transducers in the same one-dimensional direction;
the first controller is configured to control the finger period of the group of interdigital transducers in a one-dimensional direction to change in a gradient; and
the first controller and the second controller are configured to construct a two-dimensional interdigital transducer array.
4 . The gene transfection system according to claim 1 , wherein the interdigital transducer is arranged on the piezoelectric substrate, and the sound-absorbing vessel is provided on the interdigital transducer.
5 . The gene transfection system of claim 1 , wherein the bottom of the chamber is further coated with polylysine.
6 . The gene transfection system of claim 1 , wherein the piezoelectric substrate is a 128° Y-cut X-propagation, double-side polished lithium niobate substrate.