Single cell transfection with interchangeable reagent

1 . A system for single cell transfection comprising:

a transfection chamber on a transfection chip;

a fluidic channel located on the transfection chip for guiding a cell towards the transfection chamber, the fluidic channel sized to allow no more than a single cell to travel from a cell reservoir through the fluidic channel to arrive at the transfection chamber at a time;

a microfluidic ejector;

a first reagent receiver located on the transfection chip guiding a first received reagent towards the transfection chamber and intersecting with a path of the fluidic channel upstream of the transfection chamber; and

a second reagent receiver located on the transfection chip guiding a second received reagent towards the transfection chamber and intersecting with the path of the fluidic channel upstream of the transfection chamber,

wherein the first reagent receiver intersects the fluidic channel at a first distance from the transfection chamber and the second reagent receiver intersects the fluidic channel at a second distance from the transfection chamber different from the first distance,

wherein the transfection chamber comprises a constriction reducing a size of the fluidic channel such that edges of the transfection chamber apply a physical pressure on the cell within the transfection chamber, the physical pressure to porate a cell membrane of the cell to receive the first reagent from the first reagent receiver;

wherein the transfection chamber comprises a first electroporation element inside the transfection chamber on a first edge of the edges of the transfection chamber, the first edge configured to receive the cell from the fluidic channel, and a second electroporation element inside the transfection chamber on a second edge of the edges of the transfection chamber opposite the first edge of the transfection chamber, the second edge configured to output the cell to the microfluidic ejector.

2 . The system of claim 1 further comprising a microfluidic ejection chamber comprising the microfluidic ejector located on the transfection chip to eject a transfected cell, the microfluidic ejector aligned with a drop ejector orifice in the microfluidic ejection chamber.

3 . The system of claim 1 further comprising a second transfection chamber in a path between the transfection chamber on the chip and an outlet of the transfection chip.

4 . The system of claim 1 further comprising:

a second transfection chamber on the transfection chip; and

a second fluidic channel located on the transfection chip guiding a cell towards the second transfection chamber, the second fluidic channel sized to allow no more than a single cell to arrive at the second transfection chamber at a time, the fluidic channel and the second fluidic channel to connect to a cell reservoir.

5 . The system of claim 1 , wherein the microfluidic ejector is:

a first microfluidic ejector along a first outlet channel to jet fluid out of a first outlet;

and the system further comprises a second microfluidic ejector along a second outlet channel to jet fluid out of a second outlet; and

a cell analyzer configured to detect a characteristic of a cell output from the transfection chamber, the cell analyzer configured to signal activation of either the first microfluidic ejector or the second microfluidic ejector based on the characteristic detected.

6 . The system of claim 1 , wherein the constriction is within the transfection chamber.