Device used for real time field sampling for DNA extraction

1 . A device comprising:

at least one or more membrane filters, wherein each of said at least one or more membrane filters is operable for collecting one or more samples for extraction of DNA/RNA, each of said at least one or more membrane filters comprises a plurality of hollow membrane tubes or filaments being operable for filtering;

one or more filter holders, said one or more filter holders are configured to engage said at least one or more membrane filters, wherein each of said at least one or more membrane filters having a unique serial number for identification;

at least one intake pump, wherein said at least one intake pump is operable for suctioning and/or transporting said one or more samples to said at least one or more membrane filters for sample collection;

a volume controller, said volume controller is configured to control a flow of said one or more samples being transported to said one or more membrane filters, each having a unique serial number for identification for said sample collection;

wherein said volume controller determines an amount or volume of said one or more samples flowing to said one or more membrane filters;

wherein said volume controller is operable for turning said intake pump off and to turn off the flow of one or more samples, once a preprogrammed level of said one or more samples is reached;

wherein each of said at least one or more membrane filters are preserved for extraction of DNA/RNA;

wherein each of said at least one or more membrane filters further comprises a first female Luer lock connector that is configured to be operable for receiving input from said volume controller;

wherein each of said at least one or more membrane filters further comprises a second female Luer lock connector that is configured to be operable for engaging with one or more waste lines; and

an air pump coupled to an air pump switch, said air pump comprising an air compressor.

2 . The device of claim 1 , further comprising a controller switch for turning on or off an operation of said volume controller and intake pump.

3 . The device of claim 1 , further comprising a power source including at least one of, a 12-volt rechargeable lithium-ion battery, and a lithium iron phosphate battery that is configured to be operable for powering an operation of said device, wherein said intake pump engages a suction line.

4 . The device of claim 1 , wherein said air pump is operable for purging out residual sample upon activating said air pump switch.

5 . The device of claim 1 , wherein;

said first and second female Luer locks are configured to prevent a contamination of said collected samples for extraction of DNA/RNA; and

said membrane filaments having a pore size ranging from approximately 0.1 to 0.45 microns.

6 . The device of claim 1 , further comprising one or more water tubes or pipes that are configured to enable the flow and/or transport of one or more samples for extraction of DNA/RNA.

7 . The device of claim 1 , further comprising a power source including at least one of, a rechargeable battery and a solar battery configured to power an operation of said device.

8 . The device of claim 1 , further comprising a suction line, wherein said suction line is immersed at a predetermined depth into a sample source or medium for extraction of DNA/RNA.

9 . The device of claim 8 , wherein the sample comprises liquid or air and the sample source or medium comprises at least one of, an ocean, a sea, a tank, a river, and airborne debris or dust.

10 . The device of claim 1 , further comprising a priming valve, said priming valve is configured to be operable for allowing said intake pump to prime and to discharge sample waste prior to sampling.

11 . The device of claim 10 , wherein once said intake pump is primed, said priming valve is closed and the samples flows through said volume controller configured to determine and control the flow of the one or more samples being transported to said one or more membrane filters for sample collection.

12 . The device of claim 11 , wherein once a preprogrammed volume of sample is pumped into said membrane filters, the flow of one or more samples is automatically turned off and said intake pump turns off.

13 . The device of claim 12 , further comprising an air pump, said air pump comprises an air compressor, wherein upon turning off said intake pump, said air pump is turned on to purge out residual samples from said one or more membrane filters.

14 . The device of claim 13 , wherein once residual samples from said one or more membrane filters is purged out, said one or more membrane filters are injected with DNA/RNA stabilizing buffer to preserve the DNA/RNA of the collected samples and minimize the need to freeze the collected samples with liquid nitrogen, and said one or more membrane filters are closed with Luer lock end caps to avoid contamination of the collected samples.

15 . The device of claim 1 , wherein each of said at least one or more membrane filter further comprises:

an enclosure for accommodating said plurality of hollow membrane tubes or filaments, wherein said enclosure comprises at least 2-12 enclosures; and

wherein said plurality of hollow membrane tubes or filaments comprise at least 120 hollow membrane tubes or filaments.

16 . The device of claim 1 , further comprising one or more push connect polyethylene valves operable for controlling the flow of one or more samples to at least twelve membrane filters.

17 . The device of claim 1 , further comprising a programmable micro-processor, said programmable micro-processor is configured to be operable for automatic sample collection, air purging, and injection of DNA/RNA stabilizing buffer.

18 . A sampling device consisting of:

one or more sample collecting means for collecting one or more samples for extraction of DNA/RNA, each of said one or more sample collecting means comprises a plurality of hollow membrane tubes or filaments configured to be operable for filtering;

means for engaging said one or more sample collecting means, wherein each of said one or more sample collecting means further comprising a unique serial number for identification;

means for pumping or suctioning the one or more samples to said one or more sample collecting means;

means for controlling a flow of the one or more samples, the one or more samples being transported to said one or more sample collecting means;

means for determining an amount or volume of the one or more samples flowing to said one or more sample collecting means;

means for turning an intake pump off and to turn off the flow of one or more samples, once a preprogrammed level of the one or more samples is reached;

means for preserving the DNA/RNA in the one or more samples;

means for controlling an operation of at least one of said volume determining means and flow controlling means;

means for priming said pumping or suctioning means and discharge waste prior to sampling;

means for purging out residual samples from said one or more sample collection means; and

means for avoiding contamination of one or more collected samples.

19 . A device comprising:

at least one or more membrane filters, wherein each of said at least one or more membrane filters is configured to be operable for collecting one or more samples for extraction of DNA/RNA, each of said at least one or more membrane filters comprises a plurality of hollow membrane tubes or filaments being operable for filtering;

one or more filter holders, said one or more filter holders are configured to engage said at least one or more membrane filters, wherein each of said at least one or more membrane filters having a unique serial number for identification;

at least one intake pump, wherein said at least one intake pump is operable for suctioning and/or transporting said one or more samples to said at least one or more membrane filters for sample collection;

a volume controller, said volume controller is configured to control a flow of said one or more samples being transported to said one or more membrane filters;

a priming valve, said priming valve is configured to be operable for allowing said intake pump to prime and to discharge sample waste prior to sampling;

wherein said volume controller determines an amount or volume of said one or more samples flowing to said one or more membrane filters;

wherein said volume controller is operable for turning said intake pump off and to turn off the flow of samples, once a preprogrammed level of said one or more samples is reached;

wherein said at least one or more membrane filters are preserved for extraction of DNA/RNA; and

an air pump, said air pump comprising an air compressor, wherein said air pump is configured to purge out residual samples from said one or more membrane filters.

20 . The device of claim 19 , wherein once said intake pump is primed, said priming valve is closed and one or more samples flows through said volume controller, wherein said volume controller is configured to determine and control the flow of said one or more samples being transported to said one or more membrane filters for said sample collection;

wherein once a preprogrammed volume of one or more samples is pumped into said one or more membrane filters, the flow of one or more samples is automatically turned off and said intake pump turns off;

wherein upon turning off said intake pump, said air pump is turned on to purge out residual samples from said one or more membrane filters;

wherein once residual samples from said one or more membrane filters is purged out, said one or more membrane filters are injected with DNA/RNA stabilizing buffer to preserve the DNA/RNA of the collected samples and minimize the need to freeze the collected samples with liquid nitrogen; and

wherein once said one or more membrane filters are injected with DNA/RNA stabilizing buffer, said one or more membrane filters are closed with Luer lock end caps to avoid contamination of the collected samples.