Grating for optical measurements, an assembly for measurements of one or more optical parameters of a medium and a method of using the assembly

1 . A grating for optical measurements comprising:

a base plate extending in a plane in two spatial directions;

a plural number q of periodic patterns, each with a surface and space periodic wave optical mask and each with a different interval frequency;

wherein the periodic patterns are arranged adjacent each other in the base plate;

a plural number n of first periodic patterns, each with a surface and space periodic wave optical mask of the same interval frequency;

a plural number m of second periodic patterns, each with a surface and space periodic wave optical mask of the same second interval frequency;

wherein the second periodic pattern is arranged adjacent each other in the base plate with a second phase shift (ps2) between the masks of adjacent second periodic patterns;

wherein the first periodic pattern is arranged adjacent each other in the base plate with a first phase shift (ps1) between the masks of adjacent first periodic patterns; and

wherein a block of second periodic patterns is arranged adjacent a block of first periodic patterns and wherein the first interval frequency is different from the second interval frequency.

2 . The grating according to claim 1 , wherein the first phase shift (ps 1 ) is 360/n degrees.

3 . The grating according to claim 1 , wherein the periodic patterns is comprised in the group consisting of: Ronchi masks, sinusoidal masks, triangular masks, or any periodical pattern mask.

4 . The grating according to claim 3 , wherein the second phase shift (ps 2 ) is 360/m degrees.

5 . The grating according claim 1 , wherein a first periodic pattern and a second periodic pattern have a width (w) of less than 50 mm in a third spatial dimension.

6 . The grating according to claim 1 , wherein each first periodic pattern and each second periodic pattern have a width (w) of less than 10 mm in a third spatial dimension.

7 . The grating according to claim 1 , wherein each first periodic pattern and each second periodic pattern have a width (w) of less than 5 mm in a third spatial dimension.

8 . The grating according to claim 1 , wherein each first periodic pattern and each second periodic pattern have a width (w) of more than 2 mm in a third spatial dimension.

9 . A method of measuring one or more optical parameters of a medium, comprising:

using an assembly comprising:

a light profile generator configured to provide a polychromatic light profile, wherein the polychromatic light profile has a propagation path in a second spatial dimension;

a light intensity modulator configured to provide an intensity modulated polychromatic light profile by applying—to the polychromatic light profile—an intensity modulation having a periodical, or substantially periodical, pattern in a first spatial dimension;

a holder for a sample of the medium, configured to enable the intensity modulated polychromatic light sheet to illuminate the sample; and

an optical sensor configured to record intensity of light exiting the sample over a light spectrum for provision of the one or more optical parameters;

wherein light intensity modulator comprises:

an optical holder for a grating, the optical holder being electronically controlled and movable in a third spatial dimension;

a grating according to claim 1 arranged in the optical holder for providing the intensity modulation to the polychromatic light profile;

providing the holder with a sample of the medium;

iterating at least n times the steps of:

moving the optical holder of the light intensity modulator in the third spatial dimension so that light propagating in the assembly hits a next subsequent periodic pattern of the grating;

illuminating the sample by the polychromatic light sheet provided by the light sheet generator and the light intensity modulator of the assembly;

recording, by the optical sensor of the assembly, at least one measurement of intensity of light exiting the sample opposite to the illumination and intensity of light exiting the sample substantially orthogonal to the polychromatic light sheet for each of the first periodic pattern during a periodic movement of the optical holder; and

determining the one or more optical parameters based on the recorded measurements.

10 . The method according to claim 9 , wherein the light profile generator is configured to provide a polychromatic light sheet comprising a light spectrum extending in a first spatial dimension.

11 . The method according to claim 10 , wherein the recorded measurement is an image.

12 . The method according to claim 10 , wherein the iteration of the step of moving the optical holder is performed in one direction along the third spatial dimension until reaching the last position for the periodic patterns of the grating, and, after reaching the last position, change the movement direction for the next iteration to the opposite direction along the third spatial dimension until reaching a first position for the periodic patterns of the grating.

13 . The method according to claim 10 , wherein when determining the one or more optical parameter, recordings from each of the n first periodic patterns and/or each of second periodic patterns are used, or each of the q periodic patterns; and

when an iteration is performed, replacing the recorded measurement for the currently used periodic pattern with the latest recorded measurement for that periodic pattern.