Method of bioanalytical analysis utilizing ion spectrometry, including mass analysis
View Patent ↗The disclosure relates generally to analytical measurement and, more specifically, to the use of ion spectrometry, including mass analysis, for the quantitation and chemical characterization (e.g., identification) of biological or synthetic materials using two different ion spectrometric measurement cycles. The methods may be employed fruitfully in particular with analyte molecules of low molecular weight, such as metabolites.
1 . A method of bioanalytical analysis utilizing ion spectrometry, including mass analysis, comprising:
providing a pooled or otherwise composite sample which combines a plurality of individual samples, each individual sample containing a multitude of analyte compounds;
subjecting an aliquot of pooled or otherwise composite sample to a first ion spectrometric measurement cycle, which disperses the analyte compounds according to a first number of physical-chemical properties, and obtaining a first spectral dataset of the thusly dispersed analyte compounds;
evaluating the first spectral dataset and obtaining a first table of annotated features therefrom;
subjecting an aliquot of one or more individual samples from the plurality of individual samples to a second ion spectrometric measurement cycle individually, the second ion spectrometric measurement cycle dispersing the analyte compounds according to a second number of physical-chemical properties, being smaller than the first number of physical-chemical properties, and obtaining a second spectral dataset of the thusly dispersed analyte compounds for each of the one or more individual samples;
evaluating the second spectral dataset and obtaining a second table of annotated features therefrom for each of the one or more individual samples, wherein the evaluating and obtaining includes at least one of using, applying, referring to, and calling on the first table of annotated features; and
using at least one of the first table of annotated features and the second table of annotated features for one or more of the individual samples for characterizing one or more of the analyte compounds.
2 . The method of claim 1 , wherein the pooled or otherwise composite sample is provided in liquid state.
3 . The method of claim 1 , wherein the first ion spectrometric measurement cycle operates as liquid chromatography-ion mobility separation-tandem mass spectrometry (LC-IMS-MS/MS).
4 . The method of claim 3 , wherein ion mobility separation employs a gas-phase ion mobility separator taken from among the group including: travelling wave ion mobility separator (TW-IMS), trapped ion mobility separator (TIMS), field-asymmetric ion mobility separator (FAIMS), drift tube in mobility separator (DT-IMS).
5 . The method of claim 3 , wherein ion mobility separation-tandem mass spectrometry used in the first ion spectrometric measurement cycle executes a procedure of parallel accumulation and serial fragmentation (PASEF®).
6 . The method of claim 3 , wherein tandem mass spectrometry employs a mass filter, a fragmentation cell and a time-of-flight mass analyzer.
7 . The method of claim 6 , wherein the mass filter is a quadrupole mass filter.
8 . The method of claim 6 , wherein the fragmentation cell employs collision induced dissociation.
9 . The method of claim 6 , wherein the time-of-flight mass analyzer employs orthogonal acceleration.
10 . The method of claim 1 , wherein the second ion spectrometric measurement cycle operates as liquid chromatography-mass spectrometry (LC-MS).
11 . The method of claim 1 , wherein the second ion spectrometric measurement cycle operates without involving one or more measurement settings taken from among the group including: ion mobility separation, mass filtering, fragmentation.
12 . The method of claim 1 , wherein the first spectral dataset encompasses a mass/mobility map which plots abundance values as a function of mass or a mass-related parameter and mobility or a mobility-related parameter.
13 . The method of claim 1 , wherein an annotated feature in the first table of annotated features is categorized according to one or more descriptors taken from among the group including: mass m, mass-to-charge ratio m/z, isotope pattern, peak width σ, ion mobility K, collisional cross section CCS, drift time dt, retention time RT, precursor-fragment ion pattern.
14 . The method of claim 1 , wherein an annotated feature in one or more of the second tables of annotated features is categorized according to one or more descriptors taken from among the group including: mass m, mass-to-charge ratio m/z, isotope pattern, peak width σ, retention time RT.
15 . The method of claim 1 , wherein using, applying, referring to, and calling on the first table of annotated features in the obtaining a second table of annotated features includes aligning values of selected physical-chemical properties between the first spectral dataset and the corresponding second spectral dataset.
16 . The method of claim 1 , wherein using, applying, referring to, and calling on the first table of annotated features in the obtaining a second table of annotated features includes transferring information about features contained in the first table of annotated features to the corresponding second table of annotated features.
17 . The method of claim 1 , wherein the step of subjecting an aliquot of one or more individual samples from the plurality of individual samples to a second ion spectrometric measurement cycle individually includes adding measurements of quality control (QC) samples.
18 . The method of claim 17 , wherein measurement of QC samples is alternated with measurement of aliquots of individual samples.
19 . The method of claim 17 , wherein the QC samples are pooled or otherwise composite QC samples.
20 . The method of claim 1 , further comprising at least one of outputting and visualizing at least one of the first table of annotated features and one or more of the second tables of annotated features.
21 . The method of claim 20 , wherein the visualizing of a second table of annotated features includes showing those annotated features from the first table of annotated features that have been included in the obtaining of the second table of annotated features.
22 . The method of claim 1 , wherein at least one of evaluating the first spectral dataset and evaluating the second spectral dataset includes at least one of de-isotoping and de-adducting.
23 . The method of claim 1 , wherein the first table of annotated features is used for annotation and identification of analyte compounds in the pooled or otherwise composite sample.
24 . The method of claim 1 , wherein at least one of the second tables of annotated features is used for quantitation of analyte compounds in the corresponding individual sample.
25 . The method of claim 1 , wherein the analyte compounds in at least one individual sample encompass metabolites.
26 . The method of claim 1 , wherein the first ion spectrometric measurement cycle and the second ion spectrometric measurement cycle are carried out on a same analytical device which can be operated to switch the dispersing of the analyte compounds according to at least one physical-chemical property (i) on for the first ion spectrometric measurement cycle and (ii) off for the second ion spectrometric measurement cycle.