Fluorescent compound for immunohischemistry and diagnosis composition for detecting biological material comprising the same
Provided is a fluorescent compound for labeling a biomaterial having the following Chemical Formula 1: In Chemical Formula 1 above, n is an integer of 1 to 6; X 1 and X 2 are the same as or different from each other, and each independently selected from H, SO 3 − and SO 3 H; R 1 and R 2 are the same as each other or each independently selected from C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, —(CH 2 ) m1 SO 3 − , —(CH 2 ) m2 SO 3 H, and m1 is an integer of 1 to 7, m2 is an integer of 1 to 7; p1 is an integer of 1 to 10; r1 is an integer of 1 to 10; and Y 1 is selected from H, —OH, halogen and o1 is an integer of 1 to 10.
1 . A fluorescent compound for labeling a biomaterial having the following Chemical Formula 1:
In Chemical Formula 1 above,
n is an integer of 1 to 3,
X 1 and X 2 are the same as or different from each other, and each independently selected from H, —SO 3 and —SO 3 H,
either i) one of R 1 and R 2 is —(CH 2 ) m1 SO 3 − or —(CH 2 ) m2 SO 3 H, and the other one is
and R 3 and R 4 are the same or different from each other and independently selected from substituted or unsubstituted C 1-7 alkyl,
or ii) R 1 and R 2 are the same or different from each other and independently selected from —(CH 2 ) m1 SO 3 or —(CH 2 ) m2 SO 3 H, and one of R 3 and R 4 is substituted or unsubstituted C 1-7 alkyl, and the other one is
m1 is an integer of 1 to 5,
m2 is an integer of 1 to 5,
p1 is an integer of 1 to 5,
q1 is an integer of 0 to 6,
r1 is an integer of 1 to 6, and
Y 1 is selected from H, —OH, halogen and
o1 is an integer of 1 to 10,
Z 1 is selected from H, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl and
s1 is an integer of 1 to 10, and
Ar 1 is selected from H, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, C 1-7 aminoalkyl and
t1 is an integer of 1 to 10,
u1 is an integer of 1 to 10, and
R 5 is selected from H, OH, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, and
p2 is an integer of 1 to 5,
q2 is an integer of 1 to 5,
r2 is an integer of 1 to 6, and
Y 2 is selected from H, —OH, halogen and
o2 is an integer of 1 to 10,
Z 2 is selected from H, substituted or unsubstituted C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl and
s2 is an integer of 1 to 10, and
Ar 2 is selected from H, substituted or unsubstituted C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, C 1-7 aminoalkyl and
and
t2 is an integer of 1 to 4,
u2 is an integer of 1 to 4,
R 6 is selected from H, —OH, substituted or unsubstituted C 1-7 alkyl and substituted or unsubstituted C 8-20 alkyl.
2 . The fluorescent compound of claim 1 , wherein the compound of Chemical Formula 1 above is any one selected from compounds represented by the following Chemical Formulas 1-12:
3 . The fluorescent compound of claim 1 , wherein the compound is for immunohistochemistry.
4 . The fluorescent compound of claim 1 , wherein the compound is for immunohistochemistry which simultaneously stains multiple markers.
5 . An immunohistochemical composition for detecting a biomaterial comprising a fluorescent compound represented by the following Chemical Formula 1:
In Chemical Formula 1 above,
n is an integer of 1 to 3,
X 1 and X 2 are the same as or different from each other, and each independently selected from H, —SO 3 − and —SO 3 H,
either i) one of R 1 and R 2 is —(CH 2 ) m1 SO 3 or —(CH 2 ) m2 SO 3 H, and the other one is
and R 3 and R 4 are the same or different from each other and independently selected from substituted or unsubstituted C 1-7 alkyl,
or ii) R 1 and R 2 are the same or different from each other and independently selected from —(CH 2 ) m1 SO 3 or —(CH 2 ) m2 SO 3 H, and one of R 3 and R 4 should be substituted or unsubstituted C 1-7 alkyl, and the other one is
m1 is an integer of 1 to 5,
m2 is an integer of 1 to 5,
p1 is an integer of 1 to 5,
q1 is an integer of 0 to 6,
r1 is an integer of 1 to 6, and
Y 1 is selected from H, —OH, halogen and
o1 is an integer of 1 to 10,
Z 1 is selected from H, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl and
s1 is an integer of 1 to 10, and
Ar 1 is selected from H, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, C 1-7 aminoalkyl and
t1 is an integer of 1 to 10,
u1 is an integer of 1 to 10, and
R 5 is selected from H, OH, C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, and
p2 is an integer of 1 to 5,
q2 is an integer of 1 to 5,
r2 is an integer of 1 to 6, and
Y 2 is selected from H, —OH, halogen and
o2 is an integer of 1 to 10,
Z 2 is selected from H, substituted or unsubstituted C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl and
s2 is an integer of 1 to 10, and
Ar 2 is selected from H, substituted or unsubstituted C 1-7 alkyl, substituted or unsubstituted C 8-20 alkyl, C 1-7 aminoalkyl and
and
t2 is an integer of 1 to 4,
u2 is an integer of 1 to 4,
R 6 is selected from H, —OH, substituted or unsubstituted C 1-7 alkyl and substituted or unsubstituted C 8-20 alkyl.