Microfluidic flow channel structure, detection system and method for using same
The present disclosure provides microfluidic flow channel structure, detection system and method for using detection system, aiming at improving uniformity of liquid introduction of microfluidic detection system. Microfluidic flow channel structure includes liquid inlet section ( 1 ), main chamber ( 3 ) and liquid outlet section ( 2 ), main chamber ( 3 ) includes liquid inlet end ( 31 ), chamber middle part ( 33 ) and liquid outlet end ( 32 ); liquid inlet section ( 1 ), liquid inlet end ( 31 ), chamber middle part ( 33 ), liquid outlet end ( 32 ) and liquid outlet section ( 2 ) are sequentially connected together; width of liquid inlet end ( 31 ) is gradually increased in direction from liquid inlet section ( 1 ) to chamber middle part ( 33 ); thinning flow guidance region ( 310 ) is provided at edge of liquid inlet end ( 31 ) formed as width of liquid inlet end varies, and has thickness less than that of remaining region except thinning flow guidance region ( 310 ) of main chamber ( 3 ).
1 . A microfluidic flow channel structure, comprising:
a liquid inlet section, a main chamber and a liquid outlet section,
wherein the main chamber comprises a liquid inlet end, a chamber middle part and a liquid outlet end;
the liquid inlet section, the liquid inlet end, the chamber middle part, the liquid outlet end and the liquid outlet section are sequentially connected together;
a width of the liquid inlet end is gradually increased in a direction from the liquid inlet section to the chamber middle part;
the main chamber further comprises a thinning flow guidance region at an edge of the liquid inlet end formed as the width of the liquid inlet end varies, and
the thinning flow guidance region has a thickness less than that of a remaining region of the main chamber except the thinning flow guidance region,
a width of the liquid inlet section and a width of the liquid outlet section are constant and less than a width of the main chamber, a bottom surface of the liquid inlet section, a bottom surface of the main chamber and a bottom surface of the liquid outlet section are coplanar, a level of a top surface of the liquid inlet section is lower than a level of a top surface of the main chamber and a level of a top surface of the liquid outlet section, and a top surface of a portion of the main chamber other than the thinning flow guidance region is coplanar with the top surface of the liquid outlet section.
2 . The microfluidic flow channel structure according to claim 1 , wherein the liquid inlet end has a circular arc-shaped edge; and
the thinning flow guidance region is connected to the liquid inlet section and extends along the circular arc-shaped edge.
3 . The microfluidic flow channel structure according to claim 2 , wherein the liquid inlet end has a semicircular shape, and
the thinning flow guidance region is in a semicircular region at the edge of the liquid inlet end.
4 . The microfluidic flow channel structure according to claim 2 , wherein a width of the liquid outlet end is gradually reduced in a direction from the chamber middle part to the liquid outlet section.
5 . The microfluidic flow channel structure according to claim 4 , wherein the liquid outlet end has an isosceles triangle shape.
6 . The microfluidic flow channel structure according to claim 5 , wherein the chamber middle part has a square shape;
one side of two opposite sides of the square shape coincides with a chord length of the circular arc-shaped edge of the liquid inlet end, and
the other side of the two opposite sides of the square shape coincides with a bottom side of the isosceles triangle shape of the liquid outlet end.
7 . The microfluidic flow channel structure according to claim 1 , wherein a ratio of a thickness of the thinning flow guidance region to a thickness of the portion of the main chamber other than the thinning flow guidance region is 0.2 to 0.5.
8 . The microfluidic flow channel structure according to claim 1 , wherein a thickness of the thinning flow guidance region is gradually increased in a direction from an outer arc edge to an inner arc edge of the thinning flow guidance region, until the thickness of the thinning flow guidance region is equal to that of the portion of the main chamber other than the thinning flow guidance region.
9 . The microfluidic flow channel structure according to claim 1 , wherein the thinning flow guidance region has a uniform and constant thickness.
10 . The microfluidic flow channel structure according to claim 2 , wherein a thickness of the liquid inlet section is equal to the thickness of the thinning flow guidance region.
11 . The microfluidic flow channel structure according to claim 2 , wherein a ratio of the width of the thinning flow guidance region to a width of the liquid inlet section is 0.5 to 2.
12 . The microfluidic flow channel structure according to claim 1 , wherein a width of the liquid outlet section is equal to a width of the liquid inlet section.
13 . A detection system, comprising:
a detection chip and a fluorescent microscope device;
wherein the detection chip comprises the microfluidic flow channel structure according to claim 1 ; and
the fluorescent microscope device is configured to detect and read fluorescent signals in the microfluidic flow channel structure.
14 . A method for using a detection system, comprising:
fixing a first antigen or a first antibody in the microfluidic flow channel structure according to claim 1 ;
introducing a sample to be detected into the microfluidic flow channel structure, wherein the sample to be detected comprises a second antibody or a second antigen capable of specifically binding with the first antigen or the first antibody;
introducing a third antigen or a third antibody which is labeled by fluorescence into the microfluidic flow channel structure, wherein the third antigen or the third antibody b is capable of specifically binding with the second antigen or the second antibody; and
detecting and reading fluorescent signals in the microfluidic flow channel structure through a fluorescent microscope device.
15 . The microfluidic flow channel structure according to claim 3 , wherein a width of the liquid outlet end is gradually reduced in a direction from the chamber middle part to the liquid outlet section.
16 . The microfluidic flow channel structure according to claim 3 , wherein the remaining region of the main chamber except the thinning flow guidance region has a constant thickness.