Photoactive fluorescent compounds and use thereof for labeling of proteins
Provided herein are novel photoactive fluorescent compounds of Formula (A) or a salt, a single stereoisomer, a mixture of stereoisomers or an isotopic form thereof, wherein G, R, X, Z, and L A are as defined herein, and their use in labeling proteins, for example, tagged proteins, comprising their use for visualizing the location and dynamics of proteins in living cells:
1 . A compound, having a structure of Formula (IV):
wherein R 1 is —OH, —C(═O)OH, —C(═O)O(C 1-3 alkyl), —C(═O)N(C 1-3 alkyl) 2 , —N(C 1-3 alkyl) 2 , —(CH 2 ) n O(C 1-3 alkyl),
2 . The compound of claim 1 , wherein R 1 is —N(CH 3 ) 2 , —OH,
3 . The compound of claim 1 , having a structure of Formula (V):
4 . The compound of claim 2 , wherein R 1 is —N(CH 3 ) 2 , —OH,
5 . The compound of claim 1 , wherein the compound is:
6 . The compound of claim 1 , wherein the compound is:
7 . A compound having the following structure:
8 . A compound having the following structure:
9 . A compound having the following structure:
10 . A compound having the following structure: