Method for selecting a patient for a reperfusion therapy
View Patent ↗The invention relates to a method for selecting a patient suffering stroke for a reperfusion therapy based on determining the level of retinol binding protein-4 (RBP4) and N-terminal fragment of B-type natriuretic peptide (NT-proBNP) in an isolated sample of said patient. The invention also relates to a method of differentiating ischemic stroke from haemorrhagic stroke and to kits comprising reagents to carry out the methods.
1 . A method for diagnosing and treating large vessel occlusion (LVO) in a patient, said method comprising:
(a) determining the levels of RBP4 and of NT-proBNP in an isolated sample of a subject;
(b) comparing the levels of RBP4, and of NT-proBNP with a corresponding reference value, wherein said reference value or interval is selected from a value or interval of values from a reference subject suffering from LVO,
(c) diagnosing the patient with LVO when at least the levels of RBP4 and NT-proBNP are both within the value or interval of values from the reference subject suffering from LVO; and
(d) administering a reperfusion therapy comprising mechanical thrombectomy to the diagnosed patient.
2 . The method according to claim 1 , further comprising determining the level of glial fibrillary acid protein (GFAP) in the isolated sample.
3 . The method according to claim 1 , further comprising measuring one or more clinical parameters selected from blood pressure, glycemia, levels of blood d-dimer, age, scores from systematic assessment tools of stroke-related neurologic deficits, gender, and combinations thereof.
4 . The method according to claim 1 , wherein the reperfusion therapy comprises first administering an antithrombotic agent to the patient followed by mechanical thrombectomy.
5 . The method according to claim 1 , comprising determining the levels of RBP4, NT-proBNP, GFAP, and, d-dimer.
6 . A method for diagnosing and treating large vessel occlusion (LVO) in a subject, the method comprising:
(a) determining the levels of GFAP and d-dimer in an isolated sample of a subject and determining blood pressure of the subject;
(b) comparing the levels of GFAP, d-dimer, and blood pressure with a corresponding reference value, wherein said reference value or interval is selected from a value or interval of values from a reference subject suffering from LVO,
(c) diagnosing the subject with LVO when at least the levels of GFAP, d-dimer, and blood pressure are within the value or interval of values from the reference subject suffering from LVO; and
(d) administering a reperfusion therapy comprising mechanical thrombectomy to the diagnosed subject.