Composition for kidney treatment using omentum, a medical kit for kidney treatment, including the same, and film for kidney treatment, including cured product thereof
The present invention provides a composition for kidney treatment using the omentum, a medical kit for kidney treatment including the composition for kidney treatment using the omentum, and a film for kidney treatment including a cured product of the composition for kidney treatment using the omentum.
1 . A method for manufacturing of film for kidney treatment using omentum, comprising:
forming a mixture of an extracted omentum or an omentum tissue with normal saline;
grinding and filtering the mixture through a first filter having a pore diameter of 1 to 3 mm to obtain a first filtrate;
grinding and filtering the first filtrate with a second filter having a pore diameter of 400 to 800 μm to obtain a second filtrate;
grinding and filtering the second filtrate with a third filter having a pore diameter of 5 to 200 μm to obtain a third filtrate;
obtaining a micronized omentum-derived tissue extract from the third filtrate;
preparing a first liquid containing the micronized omentum-derived tissue extract and fibrinogen;
preparing a second liquid containing thrombin;
applying the first liquid to form a first layer; and
applying the second liquid onto the first layer to form a film for kidney treatment by crosslinking the fibrinogen and the thrombin;
wherein the micronized omentum-derived tissue extract comprises omentum-derived tissue particles having an average particle diameter of 5 μm to 200 μm,
wherein the micronized omentum-derived tissue extract comprises omentum-derived stromal cells, and an omentum-derived extracellular matrix, and
wherein the content of the micronized omentum-derived tissue extract in the first liquid is 40 vol % to 90 vol %.
2 . The method of claim 1 , wherein the micronized omentum-derived tissue extract is derived from autologous or allogeneic omentum.
3 . The method of claim 1 , wherein the micronized omentum-derived tissue extract is derived from great omentum.
4 . The method of claim 1 , wherein the omentum-derived tissue particles have an average particle diameter of 10 μm to 95 μm.