Molecular self-assembled nanoparticles and preparation method and application thereof
Molecular self-assembled nanoparticles and a preparation method and application thereof are provided in the present application, relating to the technical field of precise treatment of arthritis. The molecular self-assembled nanoparticles include a neutrophil membrane and aspirin-functionalized polyethylene glycol (PEG) nanoparticles, where the neutrophil membrane is coated on a surface of the aspirin-functionalized PEG nanoparticles, and a mass ratio of the neutrophil membrane to the aspirin-functionalized PEG nanoparticles is 1:8.
1 . A method for preparing molecular self-assembled nanoparticles, comprising the following steps:
step 1: dissolving aspirin and polyethylene glycol (PEG) in a mixed solvent of dichloromethane and ethyl acetate or acetone to form an oil phase, emulsifying the oil phase in an aqueous solution to obtain an oil-in-water emulsion, and drying the solvent to obtain aspirin-functionalized PEG;
step 2: dissolving the aspirin-functionalized PEG in a dimethyl sulfoxide (DMSO) solvent to obtain a mixed solution, and dropping the mixed solution into water, followed by centrifugation to obtain aspirin-functionalized PEG nanoparticles; and
step 3: dissolving the aspirin-functionalized PEG nanoparticles in a phosphate buffer solution (PBS), then dropwise adding a neutrophil membrane solution, followed by ultrasound and ice bath to obtain the molecular self-assembled nanoparticles.
2 . The preparation method according to claim 1 , wherein a mass-volume ratio of the aspirin-functionalized PEG nanoparticles to the PBS is 0.05 milligram:1 milliliter.
3 . The preparation method according to claim 1 , wherein a concentration of the neutrophil membrane solution is 4 milligrams per milliliter.