IP Library › Granted Patent US 12686857
Granted Patent B2
US 12686857 · App. 16/757,580 · Granted Jul 21, 2026

Dnase variants

Inventors: Lars Beier (Soborg, DK); Klaus Gori (Dyssegaard, DK); Lars Henrik Oestergaard (Charlottelund, DK); Lars Giger (Valby, DK); Annette Helle Johansen (Bronshoj, DK); Gernot J. Abel (Copenhagen, DK)
Assignee: Novozymes A/S
C12N9/22C12Y301/22001
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12686857
App. No.
16/757,580
Granted
Jul 21, 2026
Kind
B2
Abstract

The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Claims (69)

1 . A polypeptide variant having DNase activity, which comprises at least five substitutions selected from the group consisting of:

T1I or T1V,

G4K,

S7G,

K8R,

S9I,

S13Y,

T22P,

S25P,

S27L or S27R,

D32I, D32L, D32R, or D32V,

L33R or L33K,

S39P or S39C,

G41P,

S42G, S42H, or S42C,

Q48D,

D561 or D56L,

S57W,

S59V or S59C,

N61E,

T65L, T65V, T65M, or T65W,

S66M, S66W, S66Y, or S66V,

V76L,

T77Y,

F78L,

P91L,

S101N,

S106L, S106R, or S106H,

Q109R,

A112E,

S116D,

T127V,

S130A or S130Y,

T138D,

Q140V or Q140G,

S144P,

A147H,

C148A,

W154Y,

T157V,

Y159A or Y159R,

K160V,

G162S, G162D, or G162C,

Q166D,

S167L,

Q174N,

G175D or G175N,

L177Y,

N178D or N178E,

S179L,

C180A, and

S181E or S181L,

wherein

each position corresponds to the position of the polypeptide of SEQ ID NO: 1, and

the polypeptide variant has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1.

2 . The polypeptide variant of claim 1 , which has at least 90% sequence identity to SEQ ID NO: 1.

3 . The polypeptide variant of claim 1 , which comprises at least six of the substitutions.

4 . The polypeptide variant of claim 1 , which comprises at least seven of the substitutions.

5 . The polypeptide variant of claim 1 , which comprises at least eight of the substitutions.

6 . The polypeptide variant of claim 1 , which comprises at least nine of the substitutions.

7 . The polypeptide variant of claim 1 , which comprises at least ten of the substitutions.

8 . The polypeptide variant of claim 1 , wherein the at least five substitutions comprise at least four substitutions selected from the group consisting of T1I, S13Y, T22P, S25P, S27L, L33K, S39P, S42G, D56I, S57W, S59V, T65V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

9 . The polypeptide variant of claim 8 , comprising at least five substitutions selected from the group consisting of T1I, S13Y, T22P, S25P, S27L, L33K, S39P, S42G, D56I, S57W, S59V, T65V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

10 . The polypeptide variant of claim 8 , comprising at least six substitutions selected from the group consisting of T1I, S13Y, T22P, S25P, S27L, L33K, S39P, S42G, D56I, S57W, S59V, T65V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

11 . The polypeptide variant of claim 1 , wherein the polypeptide variant has improved detergent stability relative to the polypeptide of SEQ ID NO: 1.

12 . A detergent composition comprising the polypeptide variant of claim 1 and a surfactant.

13 . A method for laundering a textile, comprising

a) exposing the item to a wash liquor comprising the polypeptide variant of claim 1 , and

b) completing at least one wash cycle.