Sampling method

1 . A sampling method comprising:

a sampling step that includes

circulating a sample received from a cell culture device to and through a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample, and

measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;

a cleaning step that follows the sampling step, the cleaning step including

circulating a cleaning liquid from a first introduction path of the sampling system through the sampling channel, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel; and

an air introduction step that follows the cleaning step, the air introduction step including

replacing any amount of the cleaning liquid remaining in the gas concentration sensor with air from a second introduction path of the sampling system, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel,

wherein the sampling step is performed two or more times, and the second and subsequent sampling steps are performed after the air introduction step.

2 . The sampling method of claim 1 , wherein the second introduction path is opened to an atmosphere.

3 . The sampling method of claim 2 , wherein the second introduction path includes a sterile filter, and in the air introduction step, the air passes through the sterile filter before being guided to the sampling channel.

4 . The sampling method of claim 2 , wherein the sampling system further includes:

a first clamp configured to open and close the sampling channel upstream of the first connection portion relative to sample flow through the sampling channel;

a second clamp configured to open and close the first introduction path;

a third clamp configured to open and close the second introduction path; and

a pump provided in the sampling channel.

5 . The sampling method of claim 4 , wherein in the air introduction step, the air is guided from the second introduction path to the sampling channel by driving the pump in a state where the first clamp and the second clamp are closed and the third clamp is opened.

6 . The sampling method of claim 1 , wherein the sample is a first sample, the cell culture device is a first cell culture device, and the sampling channel further includes:

a sample flow path including the biosensor and the gas concentration sensor downstream of the first connection portion relative to sample flow through the sampling channel;

a first sample introduction path that guides the first sample from the first cell culture device to the sample flow path upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel and upstream of a second connection portion that joins the second introduction path and the sampling channel relative to sample flow through the sampling channel; and

a second sample introduction path that guides a second sample from a second cell culture device to the sample flow path upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel and upstream of a second connection portion that joins the second introduction path and the sampling channel relative to sample flow through the sampling channel.

7 . The sampling method of claim 6 , wherein the circulating of the sample is a first sample introduction step that circulates the first sample received from the first cell culture device to and through the sample flow path, and the sampling step further includes a second sample introduction step that includes circulating a second sample received from the second cell culture device to and through the sample flow path.

8 . The sampling method of claim 1 , wherein the sampling method further includes:

a calibrating step that includes

circulating a standard solution to and through the sampling channel, the standard solution contacting the biosensor and the gas concentration sensor.

9 . The sampling method of claim 8 , wherein the standard solution is introduced into the sampling channel from a third introduction path of the sampling sample, the third introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor.

10 . A sampling method comprising:

circulating a sample received from a cell culture device to and through a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample;

measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;

after the measuring, circulating a cleaning liquid from a first introduction path of the sampling system through the sampling channel, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel; and

after the circulation of the cleaning liquid, replacing any amounts of the cleaning liquid remaining in the gas concentration sensor with air from a second introduction path of the sampling sample, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel.

11 . The sampling method of claim 10 , wherein the second introduction path is opened to an atmosphere and the replacing of the any amounts of the cleaning liquid remaining in the gas concentration sensor with air includes moving air from the atmosphere into the sampling channel.

12 . The sampling method of claim 11 , wherein the second introduction path includes a sterile filter, and the replacing of the any amounts of the cleaning liquid remaining in the gas concentration sensor with air includes passing the air through the sterile filter before guiding the air to the sampling channel.

13 . The sampling method of claim 11 , wherein

the sampling system further includes

a first clamp configured to open and close the sampling channel upstream of the first connection portion relative to sample flow through the sampling channel,

a second clamp configured to open and close the first introduction path,

a third clamp configured to open and close the second introduction path, and

a pump provided in the sampling channel; and

the replacing of the any amounts of the cleaning liquid remaining in the gas concentration sensor with air includes driving the pump in a state where the first clamp and the second clamp are closed, and the third clamp is opened.

14 . The sampling method of claim 10 , wherein the sample is a first sample, the cell culture device is a first cell culture device, and the sampling channel further includes

a first sample introduction path that guides the first sample from the first cell culture device to the sampling channel upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel and upstream of a second connection portion that joins the second introduction path and the sampling channel relative to sample flow through the sampling channel; and

a second sample introduction path that guides a second sample from a second cell culture device to the sampling channel upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel and upstream of a second connection portion that joins the second introduction path and the sampling channel relative to sample flow through the sampling channel.

15 . The sampling method of claim 14 , wherein the circulating of the sample includes a first sample introduction step that circulates the first sample received from the first cell culture device to and through the sampling channel, and a second sample introduction step that includes circulating a second sample received from the second cell culture device to and through the sampling channel.

16 . A sampling method comprising:

receiving a sample from a cell culture device in a sampling channel of a sampling system, the sampling channel including a biosensor and a gas concentration sensor, the biosensor and the gas concentration sensor each configured to make contact with the sample;

measuring concentrations of predetermined components in the sample using the biosensor and the gas concentration sensor;

after the measuring, receiving a cleaning liquid in the sampling channel from a first introduction path of the sampling system, the cleaning liquid contacting the biosensor and the gas concentration sensor, the first introduction path connecting to the sampling channel upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel; and

after the receipt of the cleaning liquid, receiving air in the sampling channel from a second introduction path of the sampling system to replace any amounts of the cleaning liquid remaining in the gas concentration sensor, the second introduction path being separate from the first introduction path, the second introduction path connecting to the sampling channel downstream from a first connection portion that joins the first introduction path and the sampling channel and upstream of the biosensor and the gas concentration sensor relative to sample flow through the sampling channel.

17 . The sampling method of claim 16 , wherein the second introduction path is opened to an atmosphere and the receiving of the air in the sampling channel to replace any amounts of the cleaning liquid remaining in the gas concentration sensor includes guiding air from the atmosphere into the sampling channel.

18 . The sampling method of claim 17 , wherein the second introduction path includes a sterile filter, and the receiving of the air in the sampling channel to replace any amounts of the cleaning liquid remaining in the gas concentration sensor includes passing the air through the sterile filter before the guiding of the air to the sampling channel.

19 . The sampling method of claim 17 , wherein

the sampling system further includes

a first clamp configured to open and close the sampling channel upstream of the first connection portion relative to sample flow through the sampling channel,

a second clamp configured to open and close the first introduction path,

a third clamp configured to open and close the second introduction path, and

a pump provided in the sampling channel; and

the receiving of the air in the sampling channel to replace any amounts of the cleaning liquid remaining in the gas concentration sensor includes driving the pump in a state where the first clamp and the second clamp are closed, and the third clamp is opened.

20 . The sampling method of claim 16 , wherein the sample is a first sample, the cell culture device is a first cell culture device, and the sampling channel further includes:

a first sample introduction path that guides the first sample from the first cell culture device to the sampling channel upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel; and

a second sample introduction path that guides a second sample from a second cell culture device to the sampling channel upstream of both the biosensor and the gas concentration sensor relative to sample flow through the sampling channel.