Devices and methods for laser capture microdissection
A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.
1 . A system for a biological process, comprising:
(a) a sample carrier, comprising:
a cap formed from a substrate with an upper portion of the substrate comprising an outer perimeter, a first shoulder, and the top surface; an intermediate portion of the substrate, wherein the intermediate portion comprises a first seating area with a first diameter and a second shoulder; a lower portion of the substrate, wherein the lower portion comprises a second seating area with a second diameter; and a bottom surface; wherein the outer perimeter of the upper portion is larger than the first diameter of the first seating area of the intermediate portion, and wherein the first diameter is larger than the second diameter of the second seating area of the lower portion; and wherein a sample transfer film is applied to the bottom surface of the cap; and
(b) two or more sample chambers, at least one selected from each of:
(i) a first sample chamber comprising a first opening characterized by a first opening diameter; and
(ii) a second sample chamber comprising a second opening characterized by a second opening diameter that is less than the first opening diameter;
wherein the first seating area of the intermediate portion of the sample carrier is configured to engage the first opening of the first sample chamber and the second seating area of the lower portion of the sample carrier is configured to engage the second opening of the second sample chamber; and
wherein the cap is either disengaged from both the first sample chamber and the second sample chamber, or the cap is engaged with the first sample chamber, or the cap is engaged with the second sample chamber.
2 . The system of claim 1 , wherein the first sample chamber has a total capacity of 0.5 milliliters.
3 . The system of claim 1 , wherein the second sample chamber has a total capacity of 0.2 milliliters.
4 . The system of claim 1 , wherein the intermediate portion of the sample carrier comprises a first seating area with a first diameter configured to provide an interference fit within the first sample chamber opening.
5 . The system of claim 1 , wherein the lower portion of the sample carrier comprises a second seating area with a second diameter configured to provide an interference fit within the second sample chamber opening.
6 . A method of performing at least two biological processes, comprising:
providing a system according to claim 1 ;
performing a first biological process to attach a sample to the sample transfer film applied to the bottom surface of the sample carrier;
attaching a sample chamber to either the intermediate portion or the lower portion of the sample carrier;
extracting the sample from the sample transfer film; and
performing a second biological process on the sample.
7 . The method of claim 6 , wherein the first biological process is a laser capture microdissection.
8 . The method of claim 6 , wherein the first biological process is a laser capture microdissection and the second biological process is a polymerase chain reaction assay or process.
9 . The method of claim 6 , wherein the first biological process is a laser capture microdissection and the second biological process is either a real-time or digital polymerase chain reaction assay or process.
10 . The method of claim 6 , wherein the first biological process is a laser capture microdissection and the second biological process is a reverse transcriptase assay or process.
11 . The method of claim 6 , wherein the first biological process is a laser capture microdissection and the second biological process is a sequencing assay or process.
12 . The method of claim 6 , wherein the first biological process is a laser capture microdissection and the second biological process is a next generation sequencing assay or process.
13 . The method of claim 8 , further comprising a sequencing assay or process.
14 . The method of claim 10 , further comprising a sequencing assay or process.
15 . The method of claim 10 , further comprising a polymerase chain reaction assay or process and a sequencing assay or process.