Smear preparation machine and control method therefor

1 . A smear preparation machine, comprising:

a slide loading mechanism configured to load a slide;

a sample applying mechanism configured to apply a blood sample onto the slide;

a slide smearing mechanism configured to smear the blood sample on the slide to prepare a smear;

a staining mechanism comprising at least one reagent vessel and a first driving system, wherein the reagent vessel is configured to store a staining reagent and has an opening at its top, and the first driving system is configured to drive the reagent vessel to perform reciprocating linear motion in a first direction; and

a smear transferring mechanism comprising a manipulator and a second driving system, wherein the manipulator is configured to extract the smear and place the smear into the reagent vessel for staining, the second driving system is configured to drive the manipulator to perform reciprocating translation in a second direction, and the second direction is perpendicular to the first direction;

wherein the second driving system is further configured to drive the manipulator to move toward the opening of the reagent vessel to place the smear through the opening into the reagent vessel for staining or remove the smear from the reagent vessel through the opening, after the first driving system and the second driving system respectively drive the reagent vessel and the manipulator to translate to corresponding cooperative positions, and the second driving system is further configured to drive the manipulator to translate among a plurality of cooperative positions in the second direction, to place or remove the corresponding smear at the plurality of cooperative positions.

2 . The smear preparation machine of claim 1 , wherein the second driving system comprises a first driving device and a second driving device, wherein the first driving device is configured to drive the manipulator to perform reciprocating translation in the second direction, and the second driving device is configured to drive the manipulator to reciprocate in a third direction perpendicular to the second direction, so that the manipulator is moved toward the opening of the reagent vessel to place the smear into the reagent vessel for staining or remove the smear from the reagent vessel.

3 . The smear preparation machine of claim 1 , wherein the staining mechanism is provided with a smear receiving position and a storage position corresponding to the reagent vessel, wherein the smear receiving position corresponds to the cooperative positions of the reagent vessel and the manipulator, the first driving system is further configured to drive the reagent vessel to perform linear motion to move between the storage position and the smear receiving position, and the manipulator is further configured to place the smear into the reagent vessel located at the smear receiving position, or remove the smear from the reagent vessel located at the smear receiving position.

4 . The smear preparation machine of claim 3 , wherein the first driving system is further configured to drive the reagent vessel to perform reciprocating linear motion in a first horizontal plane to move between the storage position and the smear receiving position.

5 . The smear preparation machine of claim 3 , wherein the first driving system is further configured to drive the reagent vessel to perform reciprocating linear motion in the first direction so as to uniformly mix the staining reagent in the reagent vessel, and then drive the reagent vessel to move to the smear receiving position.

6 . The smear preparation machine of claim 3 , wherein the staining mechanism further comprises a base connected between the reagent vessel and the first driving system, the at least one reagent vessel comprises a plurality of reagent vessels, the staining mechanism is provided with at least one smear receiving position corresponding to each reagent vessel, and the manipulator is further configured to place or remove a smear into or from each reagent vessel at the smear receiving position corresponding to said reagent vessel.

7 . The smear preparation machine of claim 6 , wherein the plurality of reagent vessels are sequentially arranged on the base in the first direction or the second direction.

8 . The smear preparation machine of claim 1 , wherein each reagent vessel comprises at least one placing component, and each placing component is configured to provide an insertion area into which a smear is inserted, so that the smear is capable of being immersed in the staining reagent stored in the reagent vessel when the smear is inserted into the insertion area, to perform corresponding treatment.

9 . The smear preparation machine of claim 1 , wherein each reagent vessel comprises a plurality of placing components, and each placing component is configured to provide an insertion area into which a smear is inserted, so that the smear is capable of being immersed in the staining liquid stored in the reagent vessel when the smear is inserted into the insertion area, to perform corresponding treatment; and

when the plurality of reagent vessels are arranged on the base in the first direction, the plurality of placing components in each reagent vessel are arranged in the second direction; or when the plurality of reagent vessels are arranged on the base in the second direction, and the plurality of placing components in each reagent vessel are arranged in the first direction.

10 . The smear preparation machine of claim 3 , wherein the staining mechanism is provided with a fixed cover plate, which is configured to cover the opening of the reagent vessel located at the storage position.

11 . The smear preparation machine of claim 2 , wherein a moving path of the manipulator is provided with a smear taking position and a plurality of placing positions corresponding to the manipulator, the plurality of placing positions correspond to the plurality of cooperative positions of the manipulator, the first driving device is further configured to drive the manipulator to move between the smear taking position and the plurality of placing positions, and the manipulator is further configured to extract the smear at the smear taking position, and to place the smear into the reagent vessel or remove the smear from the reagent vessel at the plurality of placing positions.

12 . The smear preparation machine of claim 11 , wherein the first driving device is further configured to drive the manipulator to translate in a second horizontal plane to move between the smear taking position and the plurality of placing positions, and the second driving device is further configured to drive the manipulator to perform reciprocating translation in a vertical direction so as to place the smear into the reagent vessel or remove the smear from the reagent vessel.

13 . The smear preparation machine of claim 1 , wherein the at least one reagent vessel comprises a primary staining vessel and an isolation vessel, the primary staining vessel is configured to store a first staining liquid for primary staining of the smear, the first staining liquid is a mixture of a first biological dye and a second biological dye, the first biological dye is capable of staining acidophilic substances in the blood sample, and the second biological dye is capable of staining alkalophilic substances in the blood sample, and the isolation vessel is configured to store a reagent for treating the blood sample after the primary staining and before a secondary staining.

14 . The smear preparation machine of claim 13 , wherein the at least one reagent vessel further comprises a secondary staining vessel, which is configured to store a second staining liquid for the secondary staining of the smear, the second staining liquid is a mixture of a third biological dye and a buffer solution, and the third biological dye is capable of staining acidophilic substances or alkalophilic substances in the blood sample.

15 . The smear preparation machine of claim 13 , wherein the staining mechanism further comprises a first staining liquid recovery system, a first staining liquid inlet system, and a first staining liquid sealed container, and the first staining liquid recovery system is respectively in fluid communication with the first staining liquid sealed container and the primary staining vessel, so as to recover the corresponding first staining liquid in the primary staining vessel into the first staining liquid sealed container; and the first staining liquid inlet system is respectively in fluid communication with the first staining liquid sealed container and the primary staining vessel, so as to discharge the first staining liquid in the first staining liquid sealed container into the primary staining vessel.

16 . The smear preparation machine of claim 15 , wherein the staining mechanism further comprises a first staining liquid discharge system, which is in fluid communication with the primary staining vessel or the first staining liquid sealed container, so as to discharge system discharge the first staining liquid in the primary staining vessel or in the first staining liquid sealed container.

17 . The smear preparation machine of claim 14 , wherein the isolation vessel comprises a staining improving vessel for storing a buffer solution, and the staining improving vessel is configured to buffer and clean the smear subjected to the primary staining; or

wherein the isolation vessel further comprises a first cleaning vessel for storing a cleaning solution or a buffer solution, and the first cleaning vessel is configured to clean the smear subjected to the primary staining and/or subjected to the treatment by the staining improving vessel; or

wherein the at least one reagent vessel further comprises a second cleaning vessel for storing a cleaning solution or a buffer solution, and the second cleaning vessel is configured to clean the smear subjected to the secondary staining.

18 . A smear preparation machine, comprising:

a slide loading mechanism configured to load a slide;

a sample applying mechanism configured to apply a blood sample onto the slide;

a slide smearing mechanism configured to smear the blood sample on the slide to make a smear;

a staining mechanism, comprising at least one reagent vessel and a first driving system, wherein the reagent vessel is configured to store a staining reagent and has an opening at its top, and the first driving system is configured to drive the reagent vessel to linearly move in a single direction; and

a smear transferring mechanism comprising a manipulator and a second driving system, wherein the manipulator is configured to extract the smear and place the smear into the reagent vessel for staining, or remove the smear from the reagent vessel, and the second driving system is configured to drive the manipulator to translate among a plurality of cooperative positions in a second direction different from the single direction, to place or remove the corresponding smear at the plurality of cooperative positions.