Biochemical medical diagnostic kit

1 . A deamidation rate measurement kit comprising:

a first stock solution including deionized/distilled water containing Boc-Asn-Gly-OEt compound and

a second stock solution including deionized/distilled water containing Boc-Asp-Gly-OEt compound.

2 . The kit according to claim 1 , wherein the first stock solution has a concentration of the Boc-Asn-Gly-OEt compound in a range of 0.000001-1000 mM.

3 . The kit according to claim 2 , wherein the concentration is 0.25 mM.

4 . The kit according to claim 1 , wherein the second stock solution has a concentration of the Boc-Asp-Gly-OEt compound in a range of 0.000001-1000 mM.

5 . The kit in accordance with claim 4 , wherein the concentration is 0.25 mM.

6 . A rate measurement method of making and using the deamidation rate measurement kit in accordance claim 1 to determine a deamidation rate, the method, comprising:

making the first stock solution by serial dilutions of the Boc-Asn-Gly-OEt compound in deionized/distilled water;

making the second stock solution by serial dilutions of the Boc-Asp-Gly-OEt compound in deionized/distilled water;

creating a calibration curve on an instrument to be measured using the serial dilutions of the first Boc-Asn-Gly-OEt stock solution;

and the serial dilutions of the second Boc-Asp-Gly-OEt stock solution;

aliquoting a biological sample that has effect on the deamidation rate in equal volumes in three different sample tubes;

adding the first Boc-Asn-Gly-OEt stock solution to one of the three sample tubes, the second Boc-Asp-Gly-OEt, a standard, stock solution to a second tube of the three sample tubes, and deionized/distilled water to a third tube of the three sample tubes, in the range of 1/1000-1/2 of a final reaction volume;

making measurements and obtaining measurement results of all three sample tubes;

incubating the three sample tubes;

after incubating the three sample tubes, making and obtaining measurements of the three sample tubes; and

subtracting the measurement results of the first and second sample tubes with the added Boc-Asn-Gly-OEt and Boc-Asp-Gly-OEt from the measurement in the sample tube with the added deionized/distilled water;

determining the deamidation rate by measuring Boc-Asp-Gly-OEt, which is formed as a result of spontaneous deamidation in the first sample tube with the added Boc-Asn-Gly-OEt.

7 . The method according to claim 6 , wherein all of the measurements are made by using a LC-MS method.

8 . The method according to claim 6 , wherein the final reaction volume is 1/10.