Methods of protecting a plant from fungal pests
Phytoprotective compositions comprising one or more polypeptides having proteolytic activity in an agriculturally acceptable carrier, as well as methods of preparing such compositions and methods of using such compositions for preventing and/or treating phytopathogenic pests to reduce damage in plant propagation materials, plants and plant parts and increase crop yields.
1 . A composition comprising an effective concentration of a polypeptide in an agriculturally acceptable carrier, said polypeptide having proteolytic activity and comprising an amino acid sequence that is at least 95% identical to one or more of SEQ ID NOs: 1-5, said effective concentration sufficient to reduce or eliminate damage caused by one or more plant pathogens when said composition is applied to a plant propagation material, plant or plant part, said composition further comprising one or more pesticides.
2 . The composition of claim 1 , further comprising one or more surfactants.
3 . The composition of claim 1 , further comprising one or more nonionic surfactants.
4 . The composition of claim 1 , wherein said polypeptide comprises an amino acid sequence that is at least 96% identical to one or more of SEQ ID Nos: 1-5.
5 . The composition of claim 1 , wherein said polypeptide comprises an amino acid sequence that is at least 97% identical to one or more of SEQ ID Nos: 1-5.
6 . The composition of claim 1 , wherein said polypeptide comprises an amino acid sequence that is at least 98% identical to one or more of SEQ ID Nos: 1-5.
7 . The composition of claim 1 , wherein said polypeptide comprises an amino acid sequence that is at least 99% identical to one or more of SEQ ID Nos: 1-5.
8 . The composition of claim 1 , wherein said polypeptide comprises SEQ ID NO: 1.
9 . The composition of claim 1 , wherein said polypeptide comprises SEQ ID NO: 2.
10 . The composition of claim 1 , wherein said polypeptide comprises SEQ ID NO: 3.
11 . The composition of claim 1 , wherein said polypeptide comprises SEQ ID NO: 4.
12 . The composition of claim 1 , wherein said polypeptide comprises SEQ ID NO: 5.
13 . The composition of claim 1 , wherein said polypeptide comprises a functional proteolytic fragment of SEQ ID NO: 1.
14 . The composition of claim 1 , wherein said polypeptide comprises a functional proteolytic fragment of SEQ ID NO: 2.
15 . The composition of claim 1 , wherein said polypeptide comprises a functional proteolytic fragment of SEQ ID NO: 3.
16 . The composition of claim 1 , wherein said polypeptide comprises a functional proteolytic fragment of SEQ ID NO: 4.
17 . The composition of claim 1 , wherein said polypeptide comprises a functional proteolytic fragment of SEQ ID NO: 5.
18 . A method comprising applying the composition of claim 1 to a plant and/or plant part.
19 . A method comprising applying the composition of claim 1 to a plant growth medium.