Inhibitors of micro-RNA 22
The present disclosure provides compositions and methods that inhibit the activity of microRNAs, for example miR-22.
1 . A miR-22 inhibitory composition comprising a nucleic acid having a sequence selected from the group consisting of: G*T*t*c*T*TC*a*A*c*T*G*g*C*a*g*C*T (SEQ ID NO: 17), G*T*t*C*t*T*c*A*a*CT*g*g*c*A*g*C*T (SEQ ID NO: 19), and G*T*t*c*T*T*c*A*a*C*t*g*G*CA*g*C*T (SEQ ID NO: 20), wherein the capital letters are LNA modifications, the lower case are unmodified, the symbol * denotes PS linkages, and the absence of the * symbol denotes PO linkages.
2 . A pharmaceutical composition comprising the nucleic acid of claim 1 , and a pharmaceutically acceptable excipient or carrier.
3 . A vector or plasmid comprising a sequence encoding the nucleic acid molecule according to claim 1 .
4 . A host cell comprising the nucleic acid of claim 1 .
5 . A method for inhibiting miR-22 comprising contacting miR-22 with a miR-22 inhibitory composition of claim 1 .
6 . A method of treating obesity in a subject, the method comprising administrating the pharmaceutical composition according to claim 2 to the subject.