Plasma-treated hydrogel compositions and uses thereof

1 . A composition comprising a polymer aqueous solution, a bioceramic material comprising calcium, and reactive oxygen and nitrogen species (RONS), wherein the RONS comprises between 0.68 and 200.00 mg/L H 2 O 2 and between 13.80 and 36.80 mg/L NO 2 − .

2 . The composition according to claim 1 , wherein the RONS comprises between 12.00 and 150.00 mg/L H 2 O 2 .

3 . The composition according to claim 1 , wherein the polymer is selected from gelatin and its derivatives, fibrin, fibronectin, collagen, and collagen derivatives, alginate, agarose, cellulose, modified cellulose, xanthan gum, polyethylene glycol, hyaluronic acid, chitosan, polylactide-co-glycolide, polyhydroxyalcanoates and mixtures thereof.

4 . The composition according to claim 1 , wherein the composition comprises between 0.15 and 50.00 weight % of polymer in respect of the total weight of the composition.

5 . The composition according to claim 1 , wherein the bioceramic material comprising calcium preferably comprises calcium phosphate, and is selected from tetra-calcium phosphate, dicalcium phosphate anhydrous, dicalcium phosphate dihydrate, alpha-tricalcium phosphate, beta-tricalcium phosphate, monocalcium phosphate monohydrate, hydroxyapatite, calcium deficient hydroxyapatite, fluorapatite, amorphous calcium phosphate, calcium-sodium and potassium-phosphate, calcium- and sodium-phosphate, calcium- and potassium-phosphate, calcium pyrophosphate, calcium carbonate, calcium sulphate, calcium sulphate hemihydrate, calcium oxide and calcium hydroxide, and mixtures thereof.

6 . The composition according to claim 1 , wherein the bioceramic material is hydroxyapatite, brushite, tricalcium phosphate or mixtures thereof.

7 . The composition according to claim 1 , wherein the bioceramic material is in form of nanoparticles, microspheres, microparticles, foams or scaffolds, or mixtures thereof.

8 . The composition according to claim 1 , wherein the composition comprises between 0.5 and 99.5 weight % of bioceramic materials in respect of the total weight of the composition.

9 . The composition according to claim 1 , wherein the pH of the composition is between 5.0 and 8.0, preferably between 6.0 and 7.5, measured according to ASTM E70.

10 . The composition according to claim 1 , further comprising an active pharmaceutical ingredient.

11 . The composition according to claim 10 , wherein the active pharmaceutical ingredient is a chemotherapeutic drug or a co-adjuvant in the cancer therapy.

12 . The composition according to claim 1 , wherein the reactive oxygen and nitrogen species are generated by means of treating either the polymer aqueous solution or the polymer aqueous solution and the bioceramic material comprising calcium, with cold atmospheric plasma.

13 . The composition according to claim 1 , wherein the gelatin and its derivative is methacrylated gelatin.

14 . The composition according to claim 1 , wherein the modified cellulose is selected from hydroxypropyl cellulose, methylcellulose, carboxymethylcellulose or hydroxyethyl cellulose.

15 . The composition according to claim 1 , wherein the polymer is selected from gelatin, alginate, collagen and mixtures thereof.

16 . The composition according to claim 1 , wherein the RONS comprises between 13.60 and 150.00 mg/L H 2 O 2 and between 18.40 and 36.80 mg/L NO 2 − .

17 . A method for treating bone cancer and/or bone tissue regeneration, the method comprising the step of:

administering a composition to a patient, the composition comprising a polymer aqueous solution, a bioceramic material comprising calcium, and reactive oxygen and nitrogen species (RONS), wherein the RONS comprises between 0.68 and 200.00 mg/L H 2 O 2 and between 13.80 and 36.80 mg/L NO 2 − , as a treatment method for bone cancer or as a treatment to induce bone tissue regeneration.