Medicine for topical wound treatment
A medicine for topical wound treatment relates to the field of pharmaceutical technology. A plant composition incudes tea leaves, lotus leaves, Galla chinensis, Stevia rebaudiana, Cyclocarya paliurus, Gynura divaricata, Taraxacum mongolicum and Solanum lycopersicum . The medicine for topical wound treatment can promote the rapid repair of diabetic foot ulcers (DFUs) and reduce the damage of the DFUs to human health by controlling the local blood glucose, local infection, microvascular injury, inflammatory reaction, peripheral nerve injury, coagulation-hemolysis dysfunction of the DFUs.
1 . A preparation method of a plant extract using a plant composition for wound treatment, wherein the plant composition comprises: tea leaves, lotus leaves, Galla chinensis, Stevia rebaudiana, Cyclocarya paliurus, Gynura divaricata, Taraxacum mongolicum , and Solanum lycopersicum ; and the preparation method comprises:
step (1), crushing a mixture of the tea leaves, the lotus leaves, the Galla chinensis , the Stevia rebaudiana , the Cyclocarya paliurus , the Gynura divaricata , the Taraxacum mongolicum and the Solanum lycopersicum prepared according to a ratio to obtain a crushed mixture, wrapping the crushed mixture in a gauze and adding sodium carbonate (Na 2 CO 3 ) to obtain a mixture to be treated, followed by boiling the mixture to be treated with ethanol to collect a first extraction solution, than washing the gauze wrapped with the mixture after the boiling multiple times using the ethanol to obtain a washing solution, and adding the washing solution into the first extraction solution to obtain a first target extraction solution;
step (2), concentrating the first target extraction solution to obtain a concentrated extraction solution, cooling the concentrated extraction solution to a room temperature, performing fluid extraction 1-3 times on the concentrated extraction solution after cooling with chloroform to obtain a second extraction solution and an aqueous layer, extracting the aqueous layer 1-3 times with ethyl acetate to obtain an extraction solution of the aqueous layer, and combining the second extraction solution and the extraction solution of the aqueous layer to obtain a second target extraction solution; and
step (3), distilling the second target extraction solution under a reduced pressure until the second target extraction solution is concentrated to be a solid extract, cooling the solid extract to a room temperature, and freeze-drying the solid extract after cooling to obtain a plant extract in a form of powder.
2 . A preparation method of a plant extract using a plant composition for wound treatment, wherein the plant composition comprises: tea leaves with a mass percentage in a range of 10%-40%, lotus leaves with a mass percentage in a range of 9%-30%, Galla chinensis with a mass percentage in a range of 9%-20%, Stevia rebaudiana with a mass percentage in a range of 9%-20%, Cyclocarya paliurus with a mass percentage in a range of 9%-20%, Gynura divaricate with a mass percentage in a range of 9%-20%, Taraxacum mongolicum with a mass percentage in a range of 9%-20%, and Solanum lycopersicum with a mass percentage in a range of 5%-20%; and the preparation method comprises:
step (1), crushing a mixture of the tea leaves, the lotus leaves, the Galla chinensis , the Stevia rebaudiana , the Cyclocarya paliurus , the Gynura divaricata , the Taraxacum mongolicum and the Solanum lycopersicum prepared according to a ratio to obtain a crushed mixture, wrapping the crushed mixture in a gauze and adding sodium carbonate to obtain a mixture to be treated, followed by boiling the mixture to be treated with ethanol to collect a first extraction solution, than washing the gauze wrapped with the mixture after the boiling multiple times using the ethanol to obtain a washing solution, and adding the washing solution into the first extraction solution to obtain a first target extraction solution;
step (2), concentrating the first target extraction solution to obtain a concentrated extraction solution, cooling the concentrated extraction solution to a room temperature, performing fluid extraction 1-3 times on the concentrated extraction solution after cooling with chloroform to obtain a second extraction solution and an aqueous layer, extracting the aqueous layer 1-3 times with ethyl acetate to obtain an extraction solution of the aqueous layer, and combining the second extraction solution and the extraction solution of the aqueous layer to obtain a second target extraction solution; and
step (3), distilling the second target extraction solution under a reduced pressure until the second target extraction solution is concentrated to be a solid extract, cooling the solid extract to a room temperature, and freeze-drying the solid extract after cooling to obtain a plant extract in a form of powder.
3 . The preparation method as claimed in claim 1 , wherein a mass ratio of the crushed mixture to the sodium carbonate is in a range of 5-10:1-2.
4 . The preparation method as claimed in claim 2 , wherein a mass ratio of the crushed mixture to the sodium carbonate is in a range of 5-10:1-2.