Method for improving bone health by using mangosteen shell extract
A method for improving bone health is provided, including administering to a subject in need thereof a composition including a mangosteen shell extract. The mangosteen shell extract is prepared by performing a two-stage extraction on a fruit shell of mangosteen ( Garcinia mangostana ) using an enzyme solution at different extraction temperatures sequentially.
1 . A method for improving bone health, comprising administering to a subject in need thereof a composition comprising a mangosteen shell extract, wherein the mangosteen shell extract is prepared by performing a two-stage extraction on a fruit shell of mangosteen using an enzyme solution at different extraction temperatures sequentially.
2 . The method according to claim 1 , wherein the mangosteen shell extract has a total flavonoid content of at least 19000±5000 ppm.
3 . The method according to claim 1 , wherein the enzyme solution is an aqueous solution comprising 0.1-1 wt % of diastase.
4 . The method according to claim 3 , wherein the diastase is glucoamylase, α-amylase, β-amylase, isoamylase, or any combination thereof.
5 . The method according to claim 1 , wherein the extraction temperatures for the two-stage extraction are 45° C. to 65° C. and 75° C. to 95° C., respectively.
6 . The method according to claim 5 , wherein the extraction temperatures for the two-stage extraction are 55±5° C. and 85±5° C., respectively.
7 . The method according to claim 1 , wherein extraction time at each stage of the two-stage extraction is 60 minutes to 90 minutes.
8 . The method according to claim 1 , wherein performing the two-stage extraction comprises the following steps:
performing a primary extraction on the fruit shell of mangosteen with the enzyme solution at 45° C. to 65° C. for 60 minutes to obtain a primary extract;
performing a secondary extraction on the primary extract at 75° C. to 95° C. for 60 minutes to 90 minutes to obtain a secondary extract; and
filtering and concentrating the secondary extract to obtain the mangosteen shell extract.
9 . The method according to claim 1 , wherein the bone health is bone strengthening, joint wear and tear prevention, and/or bone loss prevention.
10 . The method according to claim 1 , wherein the mangosteen shell extract has at least one of the following functions: inhibiting osteoclast differentiation, increasing bone density, promoting intestinal calcium absorption, promoting osteocalcin secretion, and anti-inflammation, so as to strengthen the bones.
11 . The method according to claim 1 , wherein the mangosteen shell extract is used for at least one of the following functions: promoting polyglucosamine expression and reducing inflammation, so as to prevent joint wear and tear.
12 . The method according to claim 1 , wherein the mangosteen shell extract is used for at least one of the following functions: inhibiting osteoclast differentiation and promoting osteocalcin secretion, so as to prevent bone loss.
13 . The method according to claim 1 , wherein the mangosteen shell extract has a function of relieving inflammation in the body of the subject in need thereof.