IP Library › Granted Patent US 12691456
Granted Patent B2
US 12691456 · App. 17/440,216 · Granted Jul 28, 2026

System and method for manipulating objects in a fluid

Inventors: Gilad Yossifon (Mazkeret Batia, IL); Yue Wue (Haifa, IL); Sinwook Park (Haifa, IL)
Assignee: Ramot at Tel-Aviv University Ltd.
B03C5/005B03C1/01B03C1/30B03C5/026C12M35/02C12N13/00G01N33/58B03C2201/18B03C2201/26
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Quick Facts
Patent No.
US 12691456
App. No.
17/440,216
Granted
Jul 28, 2026
Kind
B2
Abstract

A method of electroporation of a biological object, comprises: introducing a carrier particle into a medium containing the biological object; applying a first electric field to the medium to induce trapping of the biological object by the carrier particle; and varying at least one parameter of the first electric field so as to induce electroporation of the biological object.

Claims (17)

1 . A method of electroporation of a biological object, comprising:

introducing a carrier particle into a medium containing the biological object;

applying a first electric field to said medium at a frequency, amplitude, and/or pulse parameter selected to induce trapping of the biological object by said carrier particle; and

varying at least said amplitude parameter so as to induce electroporation of the biological object.

2 . The method of claim 1 , comprising applying an electric field pulse train, in addition to said first electric field.

3 . The method according to claim 1 , wherein said trapping is by positive dielectrophoresis.

4 . The method according to claim 1 , wherein said trapping is by negative dielectrophoresis.

5 . The method according to claim 1 , further comprising, following said trapping and prior to said electroporation, transporting said carrier particle and the biological object to an electroporation location.

6 . The method according to claim 1 , further comprising, following said trapping and prior to said electroporation, varying a speed of said carrier particle.

7 . The method according to claim 6 , wherein said varying said speed is to a speed of zero.

8 . The method according to claim 5 , wherein said transporting comprises varying said first electric field to induce induced-charge electrophoresis or self-dielectrophoresis on said carrier particle.

9 . The method according to claim 5 , wherein the carrier particle comprises a magnetic coating or a magnetic core.

10 . The method according to claim 5 , wherein said transporting comprises applying a magnetic force or an optical field to said carrier particle.

11 . The method according to claim 1 , wherein said carrier particle is a homogenous particle.

12 . The method according to claim 1 , wherein said carrier particle is a symmetry broken particle, optionally wherein said symmetry broken particle is a Janus particle.

13 . The method according to claim 1 , wherein the biological object comprises at least one object selected from the group consisting of a bacterium, a virus, a mammalian cell, and an organelle of a biological cell.

14 . The method according to claim 1 , further comprising varying said electric field to deform the shape of the biological object, and probe at least one mechanical property thereof.