Variant ICOS ligand immunomodulatory proteins and related compositions and methods
Provided herein are immunomodulatory proteins comprising ICOSL variants and nucleic acids encoding such proteins. The immunomodulatory proteins provide therapeutic utility for a variety of immunological and oncological conditions. Compositions and methods for making and using such proteins are provided.
1 . A nucleic acid molecule encoding a variant ICOSL-Fc fusion protein comprising a variant ICOSL polypeptide linked via a linker to an Fc region, wherein the variant ICOSL polypeptide is set forth in SEQ ID NO:565, the linker is set forth in SEQ ID NO:229, and the Fc region is set forth in SEQ ID NO:637.
2 . An expression vector comprising the nucleic acid molecule of claim 1 .
3 . A cell comprising the nucleic acid molecule of claim 1 .
4 . A method of producing a variant ICOSL-Fc fusion protein, the method comprising introducing the nucleic acid molecule of claim 1 into a host cell under conditions to express the protein in the cell, and purifying the expressed protein.
5 . The method of claim 4 , wherein the cell is a CHO cell or a derivative thereof.
6 . The method of claim 5 , wherein the CHO cell or derivative thereof is a CHO DG44 cell.