IL12RB2 binding molecules and methods of use
The present disclosure relates to biologically active molecules comprising a single domain antibody (sdAb) that specifically binds to the extracellular domain of human IL12Rb2, compositions comprising such antibodies, and methods of use thereof.
1 . A IL12Rb2 binding molecule that specifically binds to the extracellular domain of IL12Rb2, wherein the IL12Rb2 binding molecule comprises a single domain antibody (sdAb), wherein the sdAb comprises a complementary determining region 1 (CDR1), a CDR2, and a CDR3 as shown in a row of the table below:
CDR1
CDR2
CDR3
FTVTRYC
IIERDGRTGY
IEGSCRP
MG
ADSVKG
DFGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 20)
NO: 21)
NO: 22)
FTISRYC
IIERDGRTGY
IEGSCRPD
MG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 23)
NO: 24)
NO: 25)
LTFDDVE
SINTDSRVYY
DPWGGDLR
MA
VDSVKD
GYPNY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 26)
NO: 27)
NO: 28)
FTISRYC
IIERDGRTGY
IEGSCRPD
MG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 29)
NO: 30)
NO: 31)
FTFSTYA
RISSGGGNTY
DDYYGGSW
MS
YADAVKG
HPIS
(SEQ ID
(SEQ ID
(SEQ ID
NO: 32)
NO: 33)
NO: 34)
YTYGLFC
VVDSPGGRHV
DPEKYCFL
MG
ADSLKG
FSDAGYQY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 35)
NO: 36)
NO: 37)
VTYSRYC
TIYSRGIITY
TRETYG
MG
YTDSVKG
GSG
(SEQ ID
(SEQ ID
DCDYESV
NO: 38)
NO: 39)
YNY
(SEQ ID
NO: 40)
FTVSRYC
IIEREGRTGY
IEGSCRPD
MG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 41)
NO: 42)
NO: 43)
FTISRYC
IIERDGRTGY
IEGSCRPD
MG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 44)
NO: 45)
NO: 46)
FTVTRYC
IIERDGRTGY
IEGSCRPD
MG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 47)
NO: 48)
NO: 49)
FTVSRY
IIERDGRTGY
IEGSCRPD
CMG
ADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 50)
NO: 51)
NO: 52)
VTYSRYCMG
TIYSRGI
TRETYGGS
(SEQ ID
ITYYTDS
GDCDYES
NO: 53)
VKG
VYNY
(SEQ ID
(SEQ ID
NO: 54)
NO: 55)
FTISKYC
IIERDGRTG
IEGSCRPD
MG
YADSVKG
FGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 56)
NO: 57)
NO: 58)
VTYSRYCMG
HIYSRGI
TRETYGGS
(SEQ ID
ITYYTDS
GDCGYESV
NO: 59)
VKG
YNY
(SEQ ID
(SEQ ID
NO: 60)
NO: 61)
FTISRYC
IIERDGRTGYA
IEGSCRP
MG
DSVKG
DLGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 62)
NO: 63)
NO: 64)
VTYSRYCMG
HIYSRGIITY
TRETYGGSGD
(SEQ ID
YTDSVKG
CSYESVYNH
NO: 65)
(SEQ ID
(SEQ ID
NO: 66)
NO: 67)
LTISRYC
IIERDGRTGYA
IEGSCRP
MG
DSVKG
DFGY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 68)
NO: 69)
NO: 70)
FTVDDFA
TISSGGSTYYA
SSVGCPL
MG
DSVKG
GY
(SEQ ID
(SEQ ID
(SEQ ID
NO: 71)
NO: 72)
NO: 73)
2 . The IL12Rb2 binding molecule of claim 1 wherein the sdAb has at least 80% identity to a polypeptide sequence of any one of SEQ ID NOS: 2-19.
3 . The IL12Rb2 binding molecule of claim 1 , wherein the sdAb is humanized or otherwise comprises CDRs grafted onto a heterologous framework.
4 . The IL12Rb2 binding molecule of claim 1 , further comprising a labeling agent, an imaging agent, and/or a therapeutic agent.