Anti-4-1BB nanobodies
Provided are anti-4-1BB nanobodies and bispecific or multispecific antibodies that incorporate the nanobodies. Methods of using the antibodies for treating and diagnosing diseases such as cancer are also provided.
1 . A single domain antibody or a polypeptide comprising the single domain antibody, wherein the single domain antibody has binding specificity to the human 4-1BB protein and comprises a complementarity determining region 1 (CDR1), a CDR2 and a CDR3, wherein the CDR1 comprises the amino acid sequence of SEQ ID NO:18, the CDR2 comprises the amino acid sequence of SEQ ID NO:58, and the CDR3 comprises the amino acid sequence of SEQ ID NO: 32.
2 . The antibody or polypeptide of claim 1 , which comprises the amino acid sequence of any one of SEQ ID NO:40-48 and 60-62.
3 . The antibody or polypeptide of claim 1 , which comprises the amino acid sequence of SEQ ID NO: 60.
4 . The antibody or polypeptide of claim 1 , wherein the polypeptide is a chimeric antigen receptor (CAR) or a bispecific antibody having a binding specificity to an antigen different from 4-1BB.
5 . A bispecific antibody comprising the antibody of claim 1 and a second antibody or antigen-binding fragment having binding specificity to a target antigen that is not 4-1BB.
6 . A composition comprising the antibody or polypeptide of claim 1 and a pharmaceutically acceptable carrier.