Polymer formulations for anti-fouling hydrogel coatings
Fouling on the surface of biomaterials and medical devices by proteins and microorganisms in the body severely hinders device functionality and drastically shortens lifetime. Currently, there is high demand for coatings that mitigate this biofouling. In this invention, the use of polyacrylamides has been explored in hydrogel coatings by building the largest library of acrylamide-based copolymer anti-biofouling hydrogels (>160 combinations) to date. A combinatorial approach was used, exploiting the ease of hydrogel synthesis to examine a high-throughput screening of platelet adhesion, precursor to thrombosis and a common culprit in biofouling. Applicability has been demonstrated of top-performing polyacrylamide-based hydrogel by (i) coating affinity-based electrochemical biosensors in vitro in a whole blood assay, and (ii) through coating an electrochemical aptamer-based device for real-time monitoring of analytes in an in vivo closed-loop system.
1 . An anti-fouling hydrogel coating, comprising:
a polymerized composition containing monomers, alone or in combinations, having a combined weight ratio of 100% with a general form of:
wherein R1 and R2 are substituents of monomers selected from the group consisting of 4-acryloylmorpholine (H), N-[3-(dimethylamino) propyl]meth-acrylamide (I) and N-isopropylacrylamide (K), wherein R1 and R2 can the same for H and I, wherein R1 and R2 cannot be the same for K.
2 . The anti-fouling hydrogel coating as set forth in claim 1 , wherein the anti-fouling hydrogel coating has a relatively better antifouling properties in terms of blood platelet adhesion, tested after protein serum adhesion, than antifouling properties in terms of blood platelet adhesion for anti-fouling hydrogels with PEG or HEMA.
3 . The anti-fouling hydrogel coating as set forth in claim 1 , further comprising a medical device, an implant or a material intended for contact with blood or bodily fluids, wherein the anti-fouling hydrogel coating is coated on the medical device, the implant or the material intended for contact with blood or bodily fluids to prevent fouling.
4 . The anti-fouling hydrogel coating as set forth in claim 1 , wherein a first monomer and a second monomer together making up the combined 100% weight ratio having individual weight ratios of at least binary combinations H, I, or K.
5 . An anti-fouling hydrogel coating, comprising:
a polymerized composition containing monomers, alone or in combinations, having a combined weight ratio of 100% with a general form of:
wherein R1 and R2 are substituents of monomers selected from the group consisting of N,N-diethylacrylamide (C), N-(3-methoxypropyl) acrylamide (D), N-hydroxymethyl-acrylamide (E), N-hydroxyethyl-acrylamide (F), N-[Tris(hydroxymethyl)methyl]acrylamide (G), 4-acryloylmorpholine (H), N-[3-(dimethylamino)propyl]methacrylamide (I), and N-isopropylacrylamide (K), wherein a first monomer and a second monomer together making up the combined 100% weight ratio having individual weight ratios of at least binary combinations C, D, E, F, G, H, I, or K, wherein R1 and R2 can the same for C, D, E, G, H and I, and wherein R1 and R2 cannot be the same for F, and K.
6 . The anti-fouling hydrogel coating as set forth in claim 5 , wherein the anti-fouling hydrogel coating has a relatively better antifouling properties in terms of blood platelet adhesion, tested after protein serum adhesion, than antifouling properties in terms of blood platelet adhesion for anti-fouling hydrogels with PEG or HEMA.
7 . The anti-fouling hydrogel coating as set forth in claim 5 , further comprising a medical device, an implant or a material intended for contact with blood or bodily fluids, wherein the anti-fouling hydrogel coating is coated on the medical device, the implant or the material intended for contact with blood or bodily fluids to prevent fouling.