Microalgae and use for same
Provided is an alga belonging to Cyanidiophyceae having a diploid cell and a haploid cell form. Also provided is a nutrient composition containing an alga belonging to Cyanidiophyceae or an extract thereof.
1 . A method for producing a haploid alga, comprising:
(a) culturing a diploid cell of an alga belonging to Galdieria or Cyanidium until the alga enters the stationary phase; and
(b) isolating a haploid cell which has no rigid cell wall from the culture obtained by (a); and
(c) culturing the haploid cell isolated in (b).
2 . The method for producing a haploid alga according to claim 1 , wherein the culturing in the (a) is performed under a condition of temperature of 30° C. to 50° C., pH of 1.0 to 5.0, and CO 2 concentration of 1% to 3%.
3 . The method for producing a haploid alga according to claim 1 , wherein the haploid cell is ruptured under a condition of pH 7.
4 . The method for producing a haploid alga according to claim 3 , wherein a ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene of the alga has a base sequence shown in SEQ ID NO: 1 or 2.
5 . The method for producing a haploid alga according to claim 4 , wherein the alga is selected from the group consisting of a Cyanidium sp. YFU3 strain (FERM BP-22334), a Cyanidium sp. HKN1 strain (FERM BP-22333).
6 . The method for producing a haploid alga according to claim 1 , wherein the alga is a transformant obtained by genetic modification using a genetic engineering technique.
7 . The method for producing a haploid alga according to claim 6 , wherein the transformant is obtained by using an auxotrophy-related gene as a selection marker.