IP Library Granted Patent US 12692473
Granted Patent B2
US 12692473 · App. 19/182,315 · Granted Jul 28, 2026

Microalgae and use for same

Inventors: Shin-Ya Miyagishima (Shizuoka, JP); Shunsuke Hirooka (Shizuoka, JP)
Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
C12N1/12A23K10/16A23L17/60A23L33/105A61K8/9717C12N1/06C12N15/74A23V2002/00
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Quick Facts
Patent No.
US 12692473
App. No.
19/182,315
Granted
Jul 28, 2026
Kind
B2
Abstract

Provided is an alga belonging to Cyanidiophyceae having a diploid cell and a haploid cell form. Also provided is a nutrient composition containing an alga belonging to Cyanidiophyceae or an extract thereof.

Claims (10)

1 . A method for producing a haploid alga, comprising:

(a) culturing a diploid cell of an alga belonging to Galdieria or Cyanidium until the alga enters the stationary phase; and

(b) isolating a haploid cell which has no rigid cell wall from the culture obtained by (a); and

(c) culturing the haploid cell isolated in (b).

2 . The method for producing a haploid alga according to claim 1 , wherein the culturing in the (a) is performed under a condition of temperature of 30° C. to 50° C., pH of 1.0 to 5.0, and CO 2 concentration of 1% to 3%.

3 . The method for producing a haploid alga according to claim 1 , wherein the haploid cell is ruptured under a condition of pH 7.

4 . The method for producing a haploid alga according to claim 3 , wherein a ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene of the alga has a base sequence shown in SEQ ID NO: 1 or 2.

5 . The method for producing a haploid alga according to claim 4 , wherein the alga is selected from the group consisting of a Cyanidium sp. YFU3 strain (FERM BP-22334), a Cyanidium sp. HKN1 strain (FERM BP-22333).

6 . The method for producing a haploid alga according to claim 1 , wherein the alga is a transformant obtained by genetic modification using a genetic engineering technique.

7 . The method for producing a haploid alga according to claim 6 , wherein the transformant is obtained by using an auxotrophy-related gene as a selection marker.