IP Library › Granted Patent US 12692480
Granted Patent B2
US 12692480 · App. 17/449,762 · Granted Jul 28, 2026

Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same

Inventors: Martin Yarmush (Newton, MA); Basak E. Uygun (Cambridge, MA); Korkut Uygun (Boston, MA); Maria-Louisa Izamis (Boston, MA); Alejandro Soto-Gutierrez (Boston, MA); Herman Tolboom (Zurich, CH); Hiroshi Yagi (Brookline, MA); Carley Shulman (Sudbury, MA); Jack Milwid (Denver, CO)
Assignees: The General Hospital Corporation; Massachusetts Institute of Technology
C12N5/067A01N1/122A01N1/143A61K35/407A61M1/14
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12692480
App. No.
17/449,762
Granted
Jul 28, 2026
Kind
B2
Abstract

One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Claims (16)

1 . A method for harvesting adult cells from an organ, the method comprising:

(a) perfusing the organ with a perfusate comprising an enzymatic digestion agent and one or more anti-inflammatory agents, wherein the one or more anti-inflammatory agents comprise hydrocortisone;

(b) isolating the adult cells from the organ; and

(c) culturing the adult cells, thereby harvesting the adult cells from the organ.

2 . The method of claim 1 , wherein the organ is a mammalian organ.

3 . The method of claim 2 , wherein the organ is a human organ.

4 . The method of claim 1 , wherein the organ is one selected from the group consisting of: spleen, heart, lung, and kidney.

5 . The method of claim 1 , wherein the organ is ischemic and/or wherein the organ is a damaged organ.

6 . The method of claim 1 , wherein the perfusate includes one or more oxygen carriers, wherein the one or more oxygen carriers comprise erythrocytes.

7 . The method of claim 1 , wherein the enzymatic digestion agent comprises collagenase.

8 . The method of claim 7 , wherein the perfusate is a normothermic perfusate, a room temperature perfusate, or a hypothermic perfusate.

9 . The method of claim 7 , further comprising providing the organ.

10 . The method of claim 7 , further comprising filtering the perfusate through one or more mesh filters having pore sizes ranging from about 1 μm to about 1000 μm.

11 . The method of claim 7 , further comprising performing density centrifugation on the perfusate, or wherein the method further comprises circulating the perfusate through a dialyzer.

12 . The method of claim 7 , wherein the perfusing step improves vitality of the adult cells.

13 . The method of claim 1 , wherein the enzymatic digestion agent is selected from the group consisting of collagenase I, collagenase II, collagenase III, collagenase IV, collagenase V, collagenase VI, trypsin, and hyaluronidase.