Method for preparing RNA derived from skin surface lipids
Provided is a method for collecting RNA contained in skin surface lipids (SSL). A method for preparing SSL-derived RNA. In this method, an aqueous solution containing RNA prepared from SSL of a subject is mixed with a water-soluble organic solvent, and the resulting mixture is allowed to contact with a solid phase material to separate RNA. By adjusting the concentration of the water-soluble organic solvent in the mixture, the yield of RNA improves.
1 . A method for preparing RNA derived from skin surface lipids, the method comprising:
preparing an aqueous solution comprising RNA, wherein the RNA is separated from skin surface lipids obtained from a subject;
mixing the aqueous solution with ethanol to prepare a mixture; and
allowing the mixture to contact with a silica-based solid phase material, so that the RNA in the mixture is adsorbed to the silica-based solid phase material,
wherein a final concentration of the ethanol in the mixture before the contact with the solid phase material is 42 vol % or more and 45 vol % or less.
2 . The method according to claim 1 , wherein the aqueous solution comprising the RNA is prepared in accordance with the phenol-chloroform method or a modified method thereof,
wherein the modified method is selected from the group consisting of a phenol-chloroform-isoamyl alcohol (PCI) method, an acid guanidinium thiocyanate-phenol-chloroform extraction (AGPC) method, and a modified AGPC method in which guanidinium thiocyanate and phenol are pre-mixed.
3 . The method according to claim 1 , wherein the silica-based solid phase material is a solid phase material having porous silica membrane.
4 . The method according to claim 1 , further comprising collecting the RNA from the silica-based solid phase material.
5 . The method according to claim 1 , wherein the skin surface lipids (SSL) are obtained from the subject using an SSL absorbent material or an SSL adhesive material.