CRISPR/Cas12F enzyme and system
The application belongs to the field of nucleic acid editing, in particular to the field of clustered regularly interspaced short palindromic repeats (CRISPR) technology. In particular, the application provides a Cas effector protein, a fusion protein with the Cas effector protein, and a nucleic acid molecule encoding the same. Also provided are a compound and a composition for nucleic acid editing (e.g., gene or genome editing) with the protein or the nucleic acid molecule, and a method for nucleic acid editing (e.g., gene or genome editing) using the protein.
1 . A delivery composition comprising a protein, wherein the protein is an effector protein in a CRISPR/Cas system and the protein comprises the amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2, and a delivery vehicle.
2 . The delivery composition of claim 1 , wherein the delivery vehicle is selected from the group consisting of a particle, a lipid particle, sugar particle, metal particle, protein particle, liposome, exosome, microvesicle, gene gun, and viral vector.
3 . The delivery composition of claim 2 , wherein the viral vector comprises replication defective retrovirus, lentivirus, adenovirus or adeno-associated virus.
4 . An in vitro, isolated or in vivo cell or cell line or progeny thereof, the cell or cell line or the progeny thereof comprising a protein, wherein the protein is an effector protein in a CRISPR/Cas system and the protein comprises the amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2, wherein the cell is a eukaryotic cell.
5 . The in vitro, isolated or in vivo cell or cell line or progeny thereof of claim 4 , wherein the cell is an animal cell or a plant cell.
6 . The in vitro, isolated or in vivo cell or cell line or progeny thereof of claim 5 , wherein the cell is a stem cell.