Microorganism having enhanced L-threonine producing ability and method for producing threonine using the same
The present application relates to a microorganism having an enhanced L-threonine producing ability and a method for producing L-threonine using the same.
1 . An L-threonine producing Corynebacterium glutamicum microorganism having enhanced glycine transporter activity and increased production of L-threonine compared to a non-modified microorganism,
wherein the microorganism comprises Corynebacterium ammoniagenes cycA gene encoding a CycA protein glycine transporter, wherein the CycA protein glycine transporter comprises the amino acid sequence of SEQ ID NO: 16.
2 . The microorganism of claim 1 , wherein the microorganism has enhanced glycine cleavage protein activity.
3 . The microorganism of claim 2 , wherein the glycine cleavage protein is at least one selected from the group consisting of GcvP, GcvT, GcvH, LipB, and LipA.
4 . The microorganism of claim 3 , wherein the glycine cleavage protein is derived from Corynebacterium ammoniagenes.
5 . The microorganism of claim 3 , wherein the GcvP comprises an amino acid sequence of SEQ ID NO: 38, GcvT comprises an amino acid sequence of SEQ ID NO: 39, GcvH comprises an amino acid sequence of SEQ ID NO: 40, LipA comprises an amino acid sequence of SEQ ID NO: 41, and LipB comprises an amino acid sequence of SEQ ID NO: 42, or an amino acid sequence having at least 90% sequence identity to the respective amino acid sequence.
6 . A composition for producing L-threonine, comprising the L-threonine producing microorganism of claim 1 .
7 . A method for producing L-threonine, comprising:
culturing the L-threonine producing microorganism of claim 1 ; and
recovering L-threonine from the microorganism or medium.