IP Library Granted Patent US 12692522
Granted Patent B2
US 12692522 · App. 17/600,594 · Granted Jul 28, 2026

Microorganism having enhanced L-threonine producing ability and method for producing threonine using the same

Inventors: Su Yon Kwon (Seoul, KR); Mina Baek (Seoul, KR); Seung-ju Son (Seoul, KR); Kwang Woo Lee (Seoul, KR)
Assignee: CJ CHEILJEDANG CORPORATION
C12P13/08C07K14/34C12N9/0016C12N9/1014C12N9/1029C12N9/18C12N15/77C12Y104/04002C12Y203/01181C12Y301/01013
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Quick Facts
Patent No.
US 12692522
App. No.
17/600,594
Granted
Jul 28, 2026
Kind
B2
Abstract

The present application relates to a microorganism having an enhanced L-threonine producing ability and a method for producing L-threonine using the same.

Claims (10)

1 . An L-threonine producing Corynebacterium glutamicum microorganism having enhanced glycine transporter activity and increased production of L-threonine compared to a non-modified microorganism,

wherein the microorganism comprises Corynebacterium ammoniagenes cycA gene encoding a CycA protein glycine transporter, wherein the CycA protein glycine transporter comprises the amino acid sequence of SEQ ID NO: 16.

2 . The microorganism of claim 1 , wherein the microorganism has enhanced glycine cleavage protein activity.

3 . The microorganism of claim 2 , wherein the glycine cleavage protein is at least one selected from the group consisting of GcvP, GcvT, GcvH, LipB, and LipA.

4 . The microorganism of claim 3 , wherein the glycine cleavage protein is derived from Corynebacterium ammoniagenes.

5 . The microorganism of claim 3 , wherein the GcvP comprises an amino acid sequence of SEQ ID NO: 38, GcvT comprises an amino acid sequence of SEQ ID NO: 39, GcvH comprises an amino acid sequence of SEQ ID NO: 40, LipA comprises an amino acid sequence of SEQ ID NO: 41, and LipB comprises an amino acid sequence of SEQ ID NO: 42, or an amino acid sequence having at least 90% sequence identity to the respective amino acid sequence.

6 . A composition for producing L-threonine, comprising the L-threonine producing microorganism of claim 1 .

7 . A method for producing L-threonine, comprising:

culturing the L-threonine producing microorganism of claim 1 ; and

recovering L-threonine from the microorganism or medium.