IP Library › Granted Patent US 12692527
Granted Patent B2
US 12692527 · App. 17/769,627 · Granted Jul 28, 2026

Glucuronosyltransferase, gene encoding same and method for using the same

Inventors: Toshiya Muranaka (Suita, JP); Hikaru Seki (Suita, JP); Soo Yeon Chung (Suita, JP); Masao Ishimoto (Tsukuba, JP); Susumu Hiraga (Tsukuba, JP); Yukiko Satou (Tsukuba, JP)
Assignees: OSAKA UNIVERSITY; NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
C12P19/56C12N1/16C12N9/1051C12N15/815C12Y204/01017C12N2800/102
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Quick Facts
Patent No.
US 12692527
App. No.
17/769,627
Granted
Jul 28, 2026
Kind
B2
Abstract

Glucuronosyltransferase 1 gene which catalyzes glucuronic acid transfer to the hydroxyl group at the 3-position in an oleanane-type triterpenoid is identified. Glucuronosyltransferase 1 gene having a desired activity, derived from a Fabaceae plant (soybean, Glycyrrhiza , and Lotus japonicus ), and containing nucleotide sequences represented by SEQ ID Nos: 2, 4, and 6, respectively, is provided.

Claims (15)

1 . A recombinant cell for producing glycyrrhizin, wherein the recombinant cell is obtained from a non-animal, capable of biologically synthesizing β-amyrin, and comprises all of the following polynucleotides (1) to (4):

(1) a polynucleotide encoding a polypeptide having an activity of oxidizing the 11-position in an oleanane-type triterpenoid and consisting of an amino acid sequence of the following (a) or (b):

(a) the amino acid sequence of SEQ ID NO:7, or

(b) an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:7;

(2) a polynucleotide encoding a polypeptide having an activity of oxidizing the 30-position in an oleanane-type triterpenoid and consisting of an amino acid sequence of the following (c) or (d):

(c) the amino acid sequence of SEQ ID NOs: 9, 11, or 13, or

(d) an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NOs: 9, 11, or 13;

(3) a polynucleotide encoding a polypeptide having an activity to transfer glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide and consisting of an amino acid sequence of the following (e) or (f):

(e) the amino acid sequence of SEQ ID NO:15, or

(f) an amino sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 15; and

(4) a polynucleotide encoding a polypeptide having an activity to transfer glucuronic acid to the hydroxy group at the 3-position in an oleanane-type triterpenoid, and consisting of an amino acid sequence of the following (g) or (h):

(g) the amino acid sequence of SEQ ID NOs: 3 or 5, or

(h) an amino acid sequence polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NOs: 3 or 5.

2 . The recombinant cell according to claim 1 , wherein the cell is from a Fabaceae plant.

3 . A method for producing glycyrrhizin from β-amyrin, comprising culturing the recombinant cell according to claim 1 .