Sensing device and method of detecting target substance

1 . A sensing device comprising:

a gas sampler that incorporates a gaseous sample or a part of a component of the sample into a liquid by causing the sample to pass through a filter structure to perform gas-liquid conversion, the filter structure having an enzyme detachably immobilized thereon, activity of the enzyme being inhibited by binding of a target substance;

an enzyme capture tank that captures an enzyme detached from the filter structure and a complex of the target substance and the enzyme detached from the filter structure;

a substrate tank that supplies a substrate solution to the enzyme capture tank; and

a sensor that performs sensing of an amount of a substrate or an amount of a product contained in the substrate solution supplied to the enzyme capture tank.

2 . The sensing device of claim 1 , wherein

the sensing is to detect a change in enzyme activity of the enzyme detached from the filter structure based on enzyme inhibition due to specific and reversible binding to the enzyme by a target substance contained in the sample.

3 . The sensing device of claim 1 , wherein

the gas sampler further comprises

a sample introduction port that receives the sample and sends the sample to the filter structure, and

a component incorporating liquid tank that accommodates a liquid sent to the filter structure to incorporate the sample or a part of the sample therein.

4 . The sensing device of claim 1 , wherein

the gas sampler further comprises an enzyme detachment mechanism that detaches the enzyme immobilized on the filter and a complex of the enzyme and a detection target, and

the enzyme detachment mechanism is a heater, electrodes, or a pH adjuster.

5 . The sensing device of any one of claim 1 to claim 4 , further comprising

a controller that controls a function of the gas sampler, the enzyme capture tank, and the substrate tank.

6 . The sensing device of any one of claim 1 to claim 4 , further comprising a mechanism that individually or collectively controls (a) conveyance of the liquid incorporating the sample or at least a part of a component of the sample, (b) application of a signal to an enzyme-immobilized tank, (c) conveyance of a substrate liquid, (d) application of a signal to the enzyme capture tank, and (e) operation of the sensor.

7 . The sensing device of any one of claim 1 to claim 4 , wherein

inhibition of enzyme activity by the target substance is inhibition due to an inhibitory action.