Marker assisted selection of traits for producing meal from

Inventors: Zoe Christina Ehlert (Saskatoon, CA); Joshua A. Flook (Indianapolis, IN); Daniel Garcia (Indianapolis, IN); Donna Carolynn Knievel (Saskatoon, CA); Cherie Ochsenfeld (Indianapolis, IN); Thomas G. Patterson (Indianapolis, IN); Ryan Preuss (Indianapolis, IN); Syed Masood Rizvi (Saskatoon, CA); Van L. Ripley (Grandora, CA); Steve Rounsley (Indianapolis, IN); Shunxue Tang (Carmel, IN); Muhammad Tahir (Saskatoon, CA); Michelle Wiggins (Westfield, IN)

Assignee: CORTEVA AGRISCIENCE LLC

C12Q1/6895 A01H1/04 A01H1/045 A01H5/10 A01H6/202 A23L19/00 C12Q2600/13 C12Q2600/158

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Quick Facts

Patent No.: US 12692555
App. No.: 18/344,018
Granted: Jul 28, 2026
Kind: B2

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Abstract

A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.

Claims (18)

1 . A method of breeding Brassica napus plants comprising an N09 chromosomal interval:

crossing a first Brassica napus plant with a second Brassica napus plant and thereby producing progeny, wherein the first plant comprises an N09 chromosomal represented by the N09 chromosomal interval of Brassica napus line YN01-429 or YN-97-262 comprising and flanked by SEQ ID NO:2 and SEQ ID NO:111;

using marker assisted selection to analyze the progeny plants or germplasm thereof for the presence or the absence of the N09 chromosomal interval comprising the plurality of markers selected from the group of SEQ ID NO:2 through SEQ ID NO:111; and

selecting one or more progeny plants or germplasm thereof having the N09 chromosomal interval, wherein the one or more selected progeny plants have lower ADF than the second Brassica napus plant.

2 . The method of claim 1 wherein the plurality of markers are selected from the following: a guanine (G) at position 501 of SEQ ID NO:2, a thymine (T) at position 501 of SEQ ID NO:3, a G at position 501 of SEQ ID NO:5, an adenine (A) at position 501 of SEQ ID NO:6, an A at position 151 of SEQ ID NO:7, an A at position 501 of SEQ ID NO:8, a 19 nucleotide insertion at position 502 of SEQ ID NO:10, a G at position 501 of SEQ ID NO:11, a G at position 501 of SEQ ID NO:12, a cytosine (C) at position 501 of SEQ ID NO:13, a thymine (T) at position 501 of SEQ ID NO:14, a guanine (G) at position 501 of SEQ ID NO:15, a cytosine (C) at position 501 of SEQ ID NO:16, a C at position 61 of SEQ ID NO:17, a G at position 501 of SEQ ID NO:18, a C at position 501 of SEQ ID NO:19, a C at position 501 of SEQ ID NO:20, a G at position 501 of SEQ ID NO:21, a C at position 501 of SEQ ID NO:22, an adenine at position 501 of SEQ ID NO:23, a C at position 501 of SEQ ID NO:24, a C at position 501 of SEQ ID NO:25, a C at position 501 of SEQ ID NO:26, an A at position 501 of SEQ ID NO:27, a TT at position 502 of SEQ ID NO:28, a T at position 501 of SEQ ID NO:29, a T at position 501 of SEQ ID NO:30, a T at position 501 of SEQ ID NO:31, a T at position 502 of SEQ ID NO:32, a T at position 501 of SEQ ID NO:34, a T at position 501 of SEQ ID NO:35, a C at position 501 of SEQ ID NO:36, a T at position 501 of SEQ ID NO:37, a T at position 501 of SEQ ID NO:38, a C at position 536 of SEQ ID NO:39, an A at position 501 of SEQ ID NO:40, an A at position 501 of SEQ ID NO:41, an A at position 501 of SEQ ID NO:42, a G at position 501 of SEQ ID NO:43, a G at position 501 of SEQ ID NO:44, an A at position 502 of SEQ ID NO:45, a T at position 501 of SEQ ID NO:46, a G at position 501 of SEQ ID NO:47, a C at position 501 of SEQ ID NO:48, an A at position 501 of SEQ ID NO:49, a T at position 501 of SEQ ID NO:50, a G at position 501 of SEQ ID NO:51, a C at position 502 of SEQ ID NO:52, a T at position 501 of SEQ ID NO:53, a T at position 501 of SEQ ID NO:54, an A at position 501 of SEQ ID NO:56, a C at position 501 of SEQ ID NO:58, a C at position 501 of SEQ ID NO:60, a C at position 501 of SEQ ID NO:61, a C at position 502 of SEQ ID NO:62, a T at position 501 of SEQ ID NO:63, an A at position 501 of SEQ ID NO:66, a C at position 501 of SEQ ID NO:67, a T at position 501 of SEQ ID NO:68, a T at position 501 of SEQ ID NO:69, an A at position 501 of SEQ ID NO:70, a T at position 501 of SEQ ID NO:72, a G at position 501 of SEQ ID NO:73, a C at position 501 of SEQ ID NO:74, an A at position 501 of SEQ ID NO:75, an A at position 501 of SEQ ID NO:76, a C at position 501 of SEQ ID NO:77, a T at position 501 of SEQ ID NO:78, a T at position 501 of SEQ ID NO:79, or a G at position 101 of SEQ ID NO:81, a G at position 61 of SEQ ID NO:82, an A at position 61 of SEQ ID NO:83, a G at position 61 of SEQ ID NO:87, a G at position 501 of SEQ ID NO:88, a G at position 101 of SEQ ID NO:89, an A at position 151 of SEQ ID NO:90, an A at position 153 of SEQ ID NO:91, a T at position 48 of SEQ ID NO:92, a C at position 61 of SEQ ID NO:93, an A at position 61 of SEQ ID NO:94, a G at position 501 of SEQ ID NO:95, a T at position 101 of SEQ ID NO:96, an A at position 151 of SEQ ID NO:97, an A at position 61 of SEQ ID NO:98, a C at position 328 of SEQ ID NO:103, a Tat position 61 of SEQ ID NO:107, a C at position 151 of SEQ ID NO:109, a Tat position 151 of SEQ ID NO:110, and a C at position 848 of SEQ ID NO:111.

3 . The method of claim 1 wherein the plurality of markers are selected from the following: a guanine (G) at position 501 of SEQ ID NO:15, a cytosine (C) at position 501 of SEQ ID NO:16, a G at position 501 of SEQ ID NO:18, a C at position 501 of SEQ ID NO:19, a C at position 501 of SEQ ID NO:20, a C at position 501 of SEQ ID NO:22, an adenine at position 501 of SEQ ID NO:23, a C at position 501 of SEQ ID NO:25, a C at position 501 of SEQ ID NO:26, a T at position 501 of SEQ ID NO:35, a T at position 501 of SEQ ID NO:38, an A at position 501 of SEQ ID NO:41, a G at position 501 of SEQ ID NO:43, a G at position 501 of SEQ ID NO:44, an A at position 502 of SEQ ID NO:45, a T at position 501 of SEQ ID NO:46, a G at position 501 of SEQ ID NO:47, a C at position 501 of SEQ ID NO:48, an A at position 501 of SEQ ID NO:49, a T at position 501 of SEQ ID NO:63, a T at position 501 of SEQ ID NO:72, a G at position 501 of SEQ ID NO:73, an A at position 501 of SEQ ID NO:75, an A at position 501 of SEQ ID NO:76, a C at position 501 of SEQ ID NO:77, a T at position 501 of SEQ ID NO:78, and a T at position 501 of SEQ ID NO:79.

4 . The method of claim 1 , further comprising using one or more of the selected progeny plants to make a Brassica napus plant line having the N09 chromosomal interval.

5 . The method of claim 2 , further comprising using one or more of the selected progeny plants to make a Brassica napus plant line having the N09 chromosomal interval.

6 . The method of claim 3 , further comprising using one or more of the selected progeny plants to make a Brassica napus plant line having the N09 chromosomal interval.

7 . The method of claim 4 , further comprising planting a field with the Brassica napus plant line having the N09 chromosomal interval, growing Brassica napus plants having the N09 chromosomal interval plants and collecting seed or grain from the grown plants.

8 . The method of claim 5 , further comprising planting a field with the Brassica napus plant line having the N09 chromosomal interval, growing Brassica napus plants having the N09 chromosomal interval plants and collecting seed or grain from the grown plants.

9 . The method of claim 6 , further comprising planting a field with the Brassica napus plant line having the N09 chromosomal interval, growing Brassica napus plants having the N09 chromosomal interval plants and collecting seed or grain from the grown plants.

10 . The method of claim 7 , further comprising producing meal from the collected seed or grain.

11 . The method of claim 8 , further comprising producing meal from the collected seed or grain.

12 . The method of claim 9 , further comprising producing meal from the collected seed or grain.

13 . The method of claim 1 further comprising, using one or more of the selected progeny plants as a parent line to produce Brassica napus seed or grain having the N09 chromosomal interval.

14 . The method of claim 2 further comprising, using one or more of the selected progeny plants as a parent line to produce Brassica napus seed or grain having the N09 chromosomal interval.

15 . The method of claim 3 further comprising, using one or more of the selected progeny plants as a parent line to produce Brassica napus seed or grain having the N09 chromosomal interval.