Low-density liquid-phase chip for
A low-density liquid-phase chip for Gossypium hirsutum L. based on targeted capture sequencing and use thereof provided. The low-density chip includes 908 SNP loci, where among the 908 SNP loci, 329 loci are significantly related to important agronomic traits, such as fiber quality, yield, and disease resistance of Gossypium hirsutum L., 14 loci are common plant transgenic detection loci, and 565 loci are other loci. This chip is adapted for use of detection of transgenic components in Gossypium hirsutum L. varieties, resource evaluation and lineage identification, seed purity identification, and genetic improvement of major agronomic traits of Gossypium hirsutum L. varieties.
1 . A liquid-phase chip for Gossypium hirsutum L. constructed by using a targeted capture sequencing technology, wherein the liquid-phase chip is a whole genome SNP chip of Gossypium hirsutum L. consisting of probes for detecting 894 SNP loci and 14 major transgenes; wherein the 14 major transgenes comprise: A1Bt, referring to Bacillus thuringiensis gene comprising of SEQ ID NO. 15; A2CPTI, referring to Cowpea Trypsin Inhibitor comprising of SEQ ID NO. 16; A3CP4-epsps, referring to 5-enolpyruvylshikimate-3-phosphate synthase gene derived from Agrobacterium sp. Cp-4 and comprising of SEQ ID NO. 17; A4CP4-epsps, referring to 5-enolpyruvylshikimate-3-phosphate synthase gene derived from Agrobacterium sp. Cp-4 2 and comprising of SEQ ID NO. 18; A5bar, referring to bialaphos resistance gene comprising of SEQ ID NO. 19; A6pat, referring to phosphinthricin acetyltransferase gene comprising of SEQ ID NO. 20; A7CdP450, referring to Cytochrome P450 comprising of SEQ ID NO. 21; A8cp4-epsps, referring to 5-enolpyruvylshikimate-3-phosphate synthase gene derived from Agrobacterium sp. Cp-4 and comprising of SEQ ID NO. 22; B.1CaMV35S, referring to 35S promoter from cauliflower mosaic virus comprising of SEQ ID NO. 23; B.2FMV35S, referring to 35S promoter from figwort mosaic virus comprising of SEQ ID NO. 24; B.3NOS, referring to promoter of nopaline synthase gene comprising of SEQ ID NO. 25; B.4NOS, referring to terminator of nopaline synthase gene comprising of SEQ ID NO. 26; B.5CaMV35S, referring to 35S terminator from the cauliflower mosaic virus comprising of SEQ ID NO. 27; and NPTII, referring to neomycin-3′-phosphotransferase gene comprising of SEQ ID NO. 28; and the 894 SNP loci comprises: SNP No. 001 at Genome position 110747518 of Chromosome A01 with Ref C and Alt T, which is a functional locus; SNP No. 002 at Genome position 11727109 of Chromosome A03 with Ref T and Alt C, which is a functional locus; SNP No. 003 at Genome position 20665441 of Chromosome A03 with Ref A and Alt G, which is a functional locus; SNP No. 004 at Genome position 2166240 of Chromosome A03 with Ref A and Alt G, which is a functional locus; SNP No. 005 at Genome position 22543857 of Chromosome A03 with Ref C and Alt T, which is a functional locus; SNP No. 006 at Genome position 8450966 of Chromosome A03 with Ref A and Alt T, which is a functional locus; SNP No. 007 at Genome position 766233 of Chromosome A05 with Ref C and Alt A, which is a functional locus; SNP No. 008 at Genome position 111125064 of Chromosome A10 with Ref A and Alt G, which is a functional locus; SNP No. 009 at Genome position 111437317 of Chromosome A10 with Ref G and Alt A, which is a functional locus; SNP No. 010 at Genome position 112338179 of Chromosome A10 with Ref A and Alt T, which is a functional locus; SNP No. 011 at Genome position 112651380 of Chromosome A10 with Ref T and Alt G, which is a functional locus; SNP No. 012 at Genome position 112940238 of Chromosome A10 with Ref G and Alt A, which is a functional locus; SNP No. 013 at Genome position 113053553 of Chromosome A10 with Ref G and Alt A, which is a functional locus; SNP No. 014 at Genome position 30523772 of Chromosome A11 with Ref A and Alt G, which is a functional locus; SNP No. 015 at Genome position 87529643 of Chromosome A12 with Ref A and Alt G, which is a functional locus; SNP No. 016 at Genome position 103027858 of Chromosome A13 with Ref C and Alt A, which is a functional locus; SNP No. 017 at Genome position 4110390 of Chromosome D02 with Ref T and Alt G, which is a functional locus; SNP No. 018 at Genome position 15331946 of Chromosome D03 with Ref G and Alt A, which is a functional locus; SNP No. 019 at Genome position 31088497 of Chromosome D03 with Ref C and Alt T, which is a functional locus; SNP No. 020 at Genome position 34610610 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 021 at Genome position 39193206 of Chromosome D03 with Ref C and Alt T, which is a functional locus; SNP No. 022 at Genome position 39600506 of Chromosome D03 with Ref A and Alt T, which is a functional locus; SNP No. 023 at Genome position 40070059 of Chromosome D03 with Ref G and Alt A, which is a functional locus; SNP No. 024 at Genome position 43229382 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 025 at Genome position 43511381 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 026 at Genome position 43821808 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 027 at Genome position 44126802 of Chromosome D03 with Ref T and Alt C, which is a functional locus; SNP No. 028 at Genome position 45101555 of Chromosome D03 with Ref G and Alt A, which is a functional locus; SNP No. 029 at Genome position 45114135 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 030 at Genome position 45162922 of Chromosome D03 with Ref A and Alt T, which is a functional locus; SNP No. 031 at Genome position 45219545 of Chromosome D03 with Ref C and Alt T, which is a functional locus; SNP No. 032 at Genome position 45904637 of Chromosome D03 with Ref A and Alt C, which is a functional locus; SNP No. 033 at Genome position 46030279 of Chromosome D03 with Ref A and Alt C, which is a functional locus; SNP No. 034 at Genome position 53296491 of Chromosome D04 with Ref T and Alt A, which is a functional locus; SNP No. 035 at Genome position 53338317 of Chromosome D04 with Ref A and Alt G, which is a functional locus; SNP No. 036 at Genome position 55873730 of Chromosome D04 with Ref G and Alt T, which is a functional locus; SNP No. 037 at Genome position 55895101 of Chromosome D04 with Ref A and Alt T, which is a functional locus; SNP No. 038 at Genome position 11366732 of Chromosome D06 with Ref G and Alt T, which is a functional locus; SNP No. 039 at Genome position 7626032 of Chromosome D09 with Ref T and Alt C, which is a functional locus; SNP No. 040 at Genome position 5613642 of Chromosome D10 with Ref C and Alt T, which is a functional locus; SNP No. 041 at Genome position 65188256 of Chromosome D10 with Ref T and Alt C, which is a functional locus; SNP No. 042 at Genome position 171062 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 043 at Genome position 23848335 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 044 at Genome position 23889334 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 045 at Genome position 23957256 of Chromosome D11 with Ref A and Alt T, which is a functional locus; SNP No. 046 at Genome position 24042912 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 047 at Genome position 9255660 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 048 at Genome position 4503413 of Chromosome D13 with Ref G and Alt A, which is a functional locus; SNP No. 049 at Genome position 103887986 of Chromosome A02 with Ref G and Alt A, which is a functional locus; SNP No. 050 at Genome position 103969684 of Chromosome A02 with Ref T and Alt C, which is a functional locus; SNP No. 051 at Genome position 103980780 of Chromosome A02 with Ref A and Alt G, which is a functional locus; SNP No. 052 at Genome position 7639601 of Chromosome A03 with Ref A and Alt G, which is a functional locus; SNP No. 053 at Genome position 4584038 of Chromosome A04 with Ref G and Alt A, which is a functional locus; SNP No. 054 at Genome position 4584101 of Chromosome A04 with Ref C and Alt T, which is a functional locus; SNP No. 055 at Genome position 69623179 of Chromosome A05 with Ref C and Alt T, which is a functional locus; SNP No. 056 at Genome position 28180129 of Chromosome A06 with Ref G and Alt G, which is a functional locus; SNP No. 057 at Genome position 28338559 of Chromosome A06 with Ref T and Alt G, which is a functional locus; SNP No. 058 at Genome position 28760155 of Chromosome A06 with Ref A and Alt G, which is a functional locus; SNP No. 059 at Genome position 37577336 of Chromosome A06 with Ref T and Alt C, which is a functional locus; SNP No. 060 at Genome position 10774519 of Chromosome A07 with Ref G and Alt A, which is a functional locus; SNP No. 061 at Genome position 89225727 of Chromosome A07 with Ref T and Alt C, which is a functional locus; SNP No. 062 at Genome position 90178382 of Chromosome A07 with Ref T and Alt C, which is a functional locus; SNP No. 063 at Genome position 90300535 of Chromosome A07 with Ref G and Alt T, which is a functional locus; SNP No. 064 at Genome position 90432981 of Chromosome A07 with Ref T and Alt C, which is a functional locus; SNP No. 065 at Genome position 90438470 of Chromosome A07 with Ref G and Alt A, which is a functional locus; SNP No. 066 at Genome position 90497386 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 067 at Genome position 4830640 of Chromosome A09 with Ref G and Alt A, which is a functional locus; SNP No. 068 at Genome position 77528103 of Chromosome A09 with Ref G and Alt T, which is a functional locus; SNP No. 069 at Genome position 109602998 of Chromosome A10 with Ref G and Alt A, which is a functional locus; SNP No. 070 at Genome position 112187864 of Chromosome A10 with Ref C and Alt A, which is a functional locus; SNP No. 071 at Genome position 112213784 of Chromosome A10 with Ref T and Alt C, which is a functional locus; SNP No. 072 at Genome position 75431311 of Chromosome A10 with Ref A and Alt G, which is a functional locus; SNP No. 073 at Genome position 75517245 of Chromosome A10 with Ref C and Alt G, which is a functional locus; SNP No. 074 at Genome position 7883291 of Chromosome A10 with Ref T and Alt C, which is a functional locus; SNP No. 075 at Genome position 114890178 of Chromosome A11 with Ref C and Alt T, which is a functional locus; SNP No. 076 at Genome position 15811782 of Chromosome A11 with Ref G and Alt A, which is a functional locus; SNP No. 077 at Genome position 23909944 of Chromosome A11 with Ref C and Alt, which is a functional locus; SNP No. 078 at Genome position 360614 of Chromosome A11 with Ref A and Alt C, which is a functional locus; SNP No. 079 at Genome position 90244406 of Chromosome A11 with Ref C and Alt A, which is a functional locus; SNP No. 080 at Genome position 57229716 of Chromosome A12 with Ref A and Alt G, which is a functional locus; SNP No. 081 at Genome position 88694205 of Chromosome A12 with Ref C and Alt T, which is a functional locus; SNP No. 082 at Genome position 90492050 of Chromosome A12 with Ref C and Alt T, which is a functional locus; SNP No. 083 at Genome position 98576530 of Chromosome A12 with Ref C and Alt T, which is a functional locus; SNP No. 084 at Genome position 105476632 of Chromosome A13 with Ref G and Alt T, which is a functional locus; SNP No. 085 at Genome position 105477213 of Chromosome A13 with Ref G and Alt A, which is a functional locus; SNP No. 086 at Genome position 105486165 of Chromosome A13 with Ref G and Alt A, which is a functional locus; SNP No. 087 at Genome position 105603690 of Chromosome A13 with Ref T and Alt C, which is a functional locus; SNP No. 088 at Genome position 64087028 of Chromosome D01 with Ref G and Alt C, which is a functional locus; SNP No. 089 at Genome position 1013903 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 090 at Genome position 1396386 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 091 at Genome position 43272859 of Chromosome D03 with Ref G and Alt C, which is a functional locus; SNP No. 092 at Genome position 43289074 of Chromosome D03 with Ref A and Alt T, which is a functional locus; SNP No. 093 at Genome position 23684485 of Chromosome D05 with Ref C and Alt A, which is a functional locus; SNP No. 094 at Genome position 5720265 of Chromosome D05 with Ref G and Alt T, which is a functional locus; SNP No. 095 at Genome position 57485276 of Chromosome D05 with Ref C and Alt A, which is a functional locus; SNP No. 096 at Genome position 58079007 of Chromosome D05 with Ref G and Alt A, which is a functional locus; SNP No. 097 at Genome position 58177617 of Chromosome D05 with Ref C and Alt T, which is a functional locus; SNP No. 098 at Genome position 12003138 of Chromosome D06 with Ref G and Alt A, which is a functional locus; SNP No. 099 at Genome position 17773574 of Chromosome D06 with Ref A and Alt G, which is a functional locus; SNP No. 100 at Genome position 60207647 of Chromosome D06 with Ref G and Alt C, which is a functional locus; SNP No. 101 at Genome position 8776619 of Chromosome D06 with Ref G and Alt, which is a functional locus; SNP No. 102 at Genome position 19075384 of Chromosome D07 with Ref A and Alt G, which is a functional locus; SNP No. 103 at Genome position 51856497 of Chromosome D07 with Ref T and Alt C, which is a functional locus; SNP No. 104 at Genome position 6729179 of Chromosome D07 with Ref A and Alt C, which is a functional locus; SNP No. 105 at Genome position 2896926 of Chromosome D08 with Ref A and Alt C, which is a functional locus; SNP No. 106 at Genome position 2901847 of Chromosome D08 with Ref A and Alt G, which is a functional locus; SNP No. 107 at Genome position 2904400 of Chromosome D08 with Ref A and Alt G, which is a functional locus; SNP No. 108 at Genome position 5505113 of Chromosome D08 with Ref G and Alt T, which is a functional locus; SNP No. 109 at Genome position 63065133 of Chromosome D08 with Ref G and Alt C, which is a functional locus; SNP No. 110 at Genome position 6549285 of Chromosome D09 with Ref G and Alt A, which is a functional locus; SNP No. 111 at Genome position 2524436 of Chromosome D10 with Ref C and Alt A, which is a functional locus; SNP No. 112 at Genome position 57041068 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 113 at Genome position 58261390 of Chromosome D11 with Ref A and Alt C, which is a functional locus; SNP No. 114 at Genome position 64752270 of Chromosome D11 with Ref C and Alt A, which is a functional locus; SNP No. 115 at Genome position 64756382 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 116 at Genome position 64904998 of Chromosome D11 with Ref G and Alt C, which is a functional locus; SNP No. 117 at Genome position 64926164 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 118 at Genome position 64930470 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 119 at Genome position 64937064 of Chromosome D11 with Ref G and Alt C, which is a functional locus; SNP No. 120 at Genome position 65065455 of Chromosome D11 with Ref C and Alt A, which is a functional locus; SNP No. 121 at Genome position 53158662 of Chromosome D12 with Ref G and Alt A, which is a functional locus; SNP No. 122 at Genome position 60059695 of Chromosome D12 with Ref T and Alt C, which is a functional locus; SNP No. 123 at Genome position 1774748 of Chromosome D13 with Ref C and Alt T, which is a functional locus; SNP No. 124 at Genome position 229536 of Chromosome D13 with Ref C and Alt G, which is a functional locus; SNP No. 125 at Genome position 45090184 of Chromosome D13 with Ref T and Alt G, which is a functional locus; SNP No. 126 at Genome position 53340754 of Chromosome D13 with Ref A and Alt C, which is a functional locus; SNP No. 127 at Genome position 8371110 of Chromosome D13 with Ref A and Alt C, which is a functional locus; SNP No. 128 at Genome position 39098148 of Chromosome seq1 with Ref T and Alt A, which is a functional locus; SNP No. 129 at Genome position 39098165 of Chromosome seq1 with Ref A and Alt C, which is a functional locus; SNP No. 130 at Genome position 39098173 of Chromosome seq1 with Ref G and Alt C, which is a functional locus; SNP No. 131 at Genome position 9660644 of Chromosome seq1 with Ref C and Alt G, which is a functional locus; SNP No. 132 at Genome position 2102346 of Chromosome A01 with Ref T and Alt C, which is a functional locus; SNP No. 133 at Genome position 8820452 of Chromosome A01 with Ref A and Alt C, which is a functional locus; SNP No. 134 at Genome position 1689682 of Chromosome A02 with Ref C and Alt T, which is a functional locus; SNP No. 135 at Genome position 51260473 of Chromosome A03 with Ref T and Alt A, which is a functional locus; SNP No. 136 at Genome position 6518897 of Chromosome A04 with Ref C and Alt T, which is a functional locus; SNP No. 137 at Genome position 11730945 of Chromosome A05 with Ref G and Alt A, which is a functional locus; SNP No. 138 at Genome position 75751203 of Chromosome A05 with Ref T and Alt C, which is a functional locus; SNP No. 139 at Genome position 8750649 of Chromosome A06 with Ref T and Alt C, which is a functional locus; SNP No. 140 at Genome position 1032070 of Chromosome A07 with Ref A and Alt C, which is a functional locus; SNP No. 141 at Genome position 1032071 of Chromosome A07 with Ref G and Alt T, which is a functional locus; SNP No. 142 at Genome position 12032478 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 143 at Genome position 95507309 of Chromosome A07 with Ref C and Alt G, which is a functional locus; SNP No. 144 at Genome position 989813 of Chromosome A07 with Ref T and Alt A, which is a functional locus; SNP No. 145 at Genome position 117118370 of Chromosome A08 with Ref A and Alt G, which is a functional locus; SNP No. 146 at Genome position 119190686 of Chromosome A08 with Ref T and Alt C, which is a functional locus; SNP No. 147 at Genome position 124176460 of Chromosome A08 with Ref A and Alt G, which is a functional locus; SNP No. 148 at Genome position 6187342 of Chromosome A08 with Ref T and Alt C, which is a functional locus; SNP No. 149 at Genome position 97074019 of Chromosome A08 with Ref C and Alt T, which is a functional locus; SNP No. 150 at Genome position 13706113 of Chromosome A09 with Ref T and Alt C, which is a functional locus; SNP No. 151 at Genome position 109236405 of Chromosome A10 with Ref A and Alt G, which is a functional locus; SNP No. 152 at Genome position 114220963 of Chromosome A10 with Ref C and Alt T, which is a functional locus; SNP No. 153 at Genome position 94150095 of Chromosome A12 with Ref C and Alt T, which is a functional locus; SNP No. 154 at Genome position 106412709 of Chromosome A13 with Ref C and Alt T, which is a functional locus; SNP No. 155 at Genome position 109879969 of Chromosome A13 with Ref C and Alt T, which is a functional locus; SNP No. 156 at Genome position 6433021 of Chromosome A13 with Ref T and Alt C, which is a functional locus; SNP No. 157 at Genome position 1150200 of Chromosome D01 with Ref T and Alt A, which is a functional locus; SNP No. 158 at Genome position 18933398 of Chromosome D01 with Ref C and Alt T, which is a functional locus; SNP No. 159 at Genome position 600285 of Chromosome D01 with Ref C and Alt T, which is a functional locus; SNP No. 160 at Genome position 12661527 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 161 at Genome position 1920733 of Chromosome D02 with Ref C and Alt G, which is a functional locus; SNP No. 162 at Genome position 1946913 of Chromosome D02 with Ref A and Alt G, which is a functional locus; SNP No. 163 at Genome position 44762375 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 164 at Genome position 6392666 of Chromosome D02 with Ref G and Alt A, which is a functional locus; SNP No. 165 at Genome position 68951786 of Chromosome D02 with Ref C and Alt A, which is a functional locus; SNP No. 166 at Genome position 2487227 of Chromosome D03 with Ref A and Alt G, which is a functional locus; SNP No. 167 at Genome position 1347244 of Chromosome D04 with Ref A and Alt G, which is a functional locus; SNP No. 168 at Genome position 52647897 of Chromosome D04 with Ref A and Alt G, which is a functional locus; SNP No. 169 at Genome position 26764654 of Chromosome D05 with Ref C and Alt T, which is a functional locus; SNP No. 170 at Genome position 45810951 of Chromosome D05 with Ref A and Alt G, which is a functional locus; SNP No. 171 at Genome position 1497967 of Chromosome D06 with Ref G and Alt A, which is a functional locus; SNP No. 172 at Genome position 1624645 of Chromosome D06 with Ref A and Alt C, which is a functional locus; SNP No. 173 at Genome position 54504155 of Chromosome D06 with Ref A and Alt T, which is a functional locus; SNP No. 174 at Genome position 59180787 of Chromosome D06 with Ref G and Alt A, which is a functional locus; SNP No. 175 at Genome position 61085437 of Chromosome D06 with Ref T and Alt A, which is a functional locus; SNP No. 176 at Genome position 46303166 of Chromosome D07 with Ref A and Alt G, which is a functional locus; SNP No. 177 at Genome position 61439658 of Chromosome D08 with Ref G and Alt A, which is a functional locus; SNP No. 178 at Genome position 68631323 of Chromosome D08 with Ref C and Alt T, which is a functional locus; SNP No. 179 at Genome position 9335830 of Chromosome D08 with Ref C and Alt T, which is a functional locus; SNP No. 180 at Genome position 2749569 of Chromosome D09 with Ref A and Alt C, which is a functional locus; SNP No. 181 at Genome position 38068242 of Chromosome D09 with Ref T and Alt C, which is a functional locus; SNP No. 182 at Genome position 1280801 of Chromosome D11 with Ref G and Alt A, which is a functional locus; SNP No. 183 at Genome position 15727440 of Chromosome D12 with Ref T and Alt C, which is a functional locus; SNP No. 184 at Genome position 58551591 of Chromosome D12 with Ref C and Alt T, which is a functional locus; SNP No. 185 at Genome position 7113678 of Chromosome D12 with Ref G and Alt A, which is a functional locus; SNP No. 186 at Genome position 59655465 of Chromosome D13 with Ref T and Alt G, which is a functional locus; SNP No. 187 at Genome position 60649746 of Chromosome D13 with Ref G and Alt A, which is a functional locus; SNP No. 188 at Genome position 110967771 of Chromosome A01 with Ref A and Alt C, which is a functional locus; SNP No. 189 at Genome position 107361717 of Chromosome A02 with Ref T and Alt A, which is a functional locus; SNP No. 190 at Genome position 1707434 of Chromosome A02 with Ref G and Alt A, which is a functional locus; SNP No. 191 at Genome position 26551420 of Chromosome A02 with Ref G and Alt A, which is a functional locus; SNP No. 192 at Genome position 14504864 of Chromosome A05 with Ref C and Alt T, which is a functional locus; SNP No. 193 at Genome position 14504889 of Chromosome A05 with Ref A and Alt G, which is a functional locus; SNP No. 194 at Genome position 15429410 of Chromosome A05 with Ref C and Alt G, which is a functional locus; SNP No. 195 at Genome position 42902490 of Chromosome A07 with Ref C and Alt G, which is a functional locus; SNP No. 196 at Genome position 90603512 of Chromosome A07 with Ref T and Alt G, which is a functional locus; SNP No. 197 at Genome position 90783146 of Chromosome A07 with Ref G and Alt A, which is a functional locus; SNP No. 198 at Genome position 112071927 of Chromosome A08 with Ref C and Alt T, which is a functional locus; SNP No. 199 at Genome position 110613300 of Chromosome A10 with Ref T and Alt C, which is a functional locus; SNP No. 200 at Genome position 104539963 of Chromosome A11 with Ref T and Alt C, which is a functional locus; SNP No. 201 at Genome position 1913626 of Chromosome D02 with Ref A and Alt G, which is a functional locus; SNP No. 202 at Genome position 1915388 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 203 at Genome position 6362048 of Chromosome D02 with Ref C and Alt T, which is a functional locus; SNP No. 204 at Genome position 1546736 of Chromosome D04 with Ref T and Alt A, which is a functional locus; SNP No. 205 at Genome position 14240130 of Chromosome D05 with Ref T and Alt G, which is a functional locus; SNP No. 206 at Genome position 45390691 of Chromosome D06 with Ref C and Alt T, which is a functional locus; SNP No. 207 at Genome position 46672551 of Chromosome D06 with Ref C and Alt T, which is a functional locus; SNP No. 208 at Genome position 18841330 of Chromosome D08 with Ref A and Alt C, which is a functional locus; SNP No. 209 at Genome position 20428933 of Chromosome D11 with Ref A and Alt T, which is a functional locus; SNP No. 210 at Genome position 114857126 of Chromosome A01 with Ref G and Alt A, which is a functional locus; SNP No. 211 at Genome position 115721972 of Chromosome A01 with Ref A and Alt G, which is a functional locus; SNP No. 212 at Genome position 117760175 of Chromosome A01 with Ref T and Alt C, which is a functional locus; SNP No. 213 at Genome position 117760658 of Chromosome A01 with Ref T and Alt A, which is a functional locus; SNP No. 214 at Genome position 106224475 of Chromosome A02 with Ref A and Alt G, which is a functional locus; SNP No. 215 at Genome position 3111851 of Chromosome A02 with Ref A and Alt G, which is a functional locus; SNP No. 216 at Genome position 110233891 of Chromosome A03 with Ref A and Alt G, which is a functional locus; SNP No. 217 at Genome position 67550448 of Chromosome A04 with Ref A and Alt T, which is a functional locus; SNP No. 218 at Genome position 86560855 of Chromosome A04 with Ref C and Alt T, which is a functional locus; SNP No. 219 at Genome position 86568861 of Chromosome A04 with Ref C and Alt T, which is a functional locus; SNP No. 220 at Genome position 105715349 of Chromosome A05 with Ref A and Alt G, which is a functional locus; SNP No. 221 at Genome position 15876258 of Chromosome A05 with Ref T and Alt C, which is a functional locus; SNP No. 222 at Genome position 15876308 of Chromosome A05 with Ref C and Alt G, which is a functional locus; SNP No. 223 at Genome position 1874595 of Chromosome A05 with Ref A and Alt G, which is a functional locus; SNP No. 224 at Genome position 2124121 of Chromosome A05 with Ref C and Alt T, which is a functional locus; SNP No. 225 at Genome position 22775565 of Chromosome A05 with Ref G and Alt A, which is a functional locus; SNP No. 226 at Genome position 22924264 of Chromosome A05 with Ref T and Alt A, which is a functional locus; SNP No. 227 at Genome position 22938538 of Chromosome A05 with Ref A and Alt T, which is a functional locus; SNP No. 228 at Genome position 31423050 of Chromosome A05 with Ref T and Alt C, which is a functional locus; SNP No. 229 at Genome position 119255407 of Chromosome A06 with Ref C and Alt T, which is a functional locus; SNP No. 230 at Genome position 124093748 of Chromosome A06 with Ref C and Alt T, which is a functional locus; SNP No. 231 at Genome position 1503775 of Chromosome A06 with Ref C and Alt G, which is a functional locus; SNP No. 232 at Genome position 21563371 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 233 at Genome position 23460168 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 234 at Genome position 83188617 of Chromosome A07 with Ref G and Alt C, which is a functional locus; SNP No. 235 at Genome position 88838111 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 236 at Genome position 90625054 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 237 at Genome position 90633925 of Chromosome A07 with Ref C and Alt T, which is a functional locus; SNP No. 238 at Genome position 90639206 of Chromosome A07 with Ref T and Alt C, which is a functional locus; SNP No. 239 at Genome position 104027372 of Chromosome A08 with Ref A and Alt G, which is a functional locus; SNP No. 240 at Genome position 107661059 of Chromosome A08 with Ref T and Alt G, which is a functional locus; SNP No. 241 at Genome position 123086263 of Chromosome A08 with Ref C and Alt G, which is a functional locus; SNP No. 242 at Genome position 123101111 of Chromosome A08 with Ref T and Alt C, which is a functional locus; SNP No. 243 at Genome position 31131479 of Chromosome A08 with Ref C and Alt T, which is a functional locus; SNP No. 244 at Genome position 6469161 of Chromosome A08 with Ref T and Alt A, which is a functional locus; SNP No. 245 at Genome position 73717101 of Chromosome A09 with Ref C and Alt A, which is a functional locus; SNP No. 246 at Genome position 108187386 of Chromosome A10 with Ref C and Alt T, which is a functional locus; SNP No. 247 at Genome position 114006682 of Chromosome A10 with Ref G and Alt A, which is a functional locus; SNP No. 248 at Genome position 2516523 of Chromosome A10 with Ref C and Alt T, which is a functional locus; SNP No. 249 at Genome position 4309496 of Chromosome A10 with Ref C and Alt A, which is a functional locus; SNP No. 250 at Genome position 118950829 of Chromosome A11 with Ref A and Alt T, which is a functional locus; SNP No. 251 at Genome position 24222 of Chromosome A11 with Ref G and Alt A, which is a functional locus; SNP No. 252 at Genome position 9073286 of Chromosome A11 with Ref G and Alt A, which is a functional locus; SNP No. 253 at Genome position 99926974 of Chromosome A11 with Ref A and Alt G, which is a functional locus; SNP No. 254 at Genome position 104493499 of Chromosome A12 with Ref T and Alt A, which is a functional locus; SNP No. 255 at Genome position 95983460 of Chromosome A12 with Ref C and Alt G, which is a functional locus; SNP No. 256 at Genome position 100671788 of Chromosome A13 with Ref C and Alt T, which is a functional locus; SNP No. 257 at Genome position 104864916 of Chromosome A13 with Ref T and Alt G, which is a functional locus; SNP No. 258 at Genome position 107008682 of Chromosome A13 with Ref A and Alt G, which is a functional locus; SNP No. 259 at Genome position 5759232 of Chromosome A13 with Ref A and Alt G, which is a functional locus; SNP No. 260 at Genome position 68442801 of Chromosome A13 with Ref C and Alt A, which is a functional locus; SNP No. 261 at Genome position 89442130 of Chromosome A13 with Ref G and Alt A, which is a functional locus; SNP No. 262 at Genome position 98283310 of Chromosome A13 with Ref T and Alt C, which is a functional locus; SNP No. 263 at Genome position 19034036 of Chromosome D01 with Ref T and Alt A, which is a functional locus; SNP No. 264 at Genome position 54855218 of Chromosome D01 with Ref T and Alt C, which is a functional locus; SNP No. 265 at Genome position 54866986 of Chromosome D01 with Ref C and Alt T, which is a functional locus; SNP No. 266 at Genome position 54890106 of Chromosome D01 with Ref T and Alt C, which is a functional locus; SNP No. 267 at Genome position 54896281 of Chromosome D01 with Ref G and Alt A, which is a functional locus; SNP No. 268 at Genome position 57261588 of Chromosome D01 with Ref A and Alt G, which is a functional locus; SNP No. 269 at Genome position 58881229 of Chromosome D01 with Ref C and Alt T, which is a functional locus; SNP No. 270 at Genome position 51851185 of Chromosome D02 with Ref A and Alt G, which is a functional locus; SNP No. 271 at Genome position 52987299 of Chromosome D02 with Ref A and Alt G, which is a functional locus; SNP No. 272 at Genome position 53034743 of Chromosome D02 with Ref G and Alt T, which is a functional locus; SNP No. 273 at Genome position 53253589 of Chromosome D02 with Ref A and Alt C, which is a functional locus; SNP No. 274 at Genome position 54901956 of Chromosome D02 with Ref C and Alt T, which is a functional locus; SNP No. 275 at Genome position 63310106 of Chromosome D02 with Ref T and Alt C, which is a functional locus; SNP No. 276 at Genome position 6401616 of Chromosome D02 with Ref T and Alt A, which is a functional locus; SNP No. 277 at Genome position 68030533 of Chromosome D02 with Ref A and Alt T, which is a functional locus; SNP No. 278 at Genome position 7489387 of Chromosome D02 with Ref T and Alt G, which is a functional locus; SNP No. 279 at Genome position 7531127 of Chromosome D02 with Ref A and Alt G, which is a functional locus; SNP No. 280 at Genome position 2503186 of Chromosome D03 with Ref G and Alt T, which is a functional locus; SNP No. 281 at Genome position 52350838 of Chromosome D04 with Ref T and Alt G, which is a functional locus; SNP No. 282 at Genome position 6848742 of Chromosome D04 with Ref A and Alt C, which is a functional locus; SNP No. 283 at Genome position 6861687 of Chromosome D04 with Ref C and Alt T, which is a functional locus; SNP No. 284 at Genome position 851577 of Chromosome D04 with Ref G and Alt A, which is a functional locus; SNP No. 285 at Genome position of Chromosome D05 with 14718765 Ref A and Alt G, which is a functional locus; SNP No. 286 at Genome position of Chromosome D05 with 55154526 Ref T and Alt C, which is a functional locus; SNP No. 287 at Genome position 56805649 of Chromosome D05 with Ref A and Alt G, which is a functional locus; SNP No. 288 at Genome position 18372518 of Chromosome D06 with Ref T and Alt A, which is a functional locus; SNP No. 289 at Genome position 47311286 of Chromosome D06 with Ref G and Alt T, which is a functional locus; SNP No. 290 at Genome position 64318600 of Chromosome D06 with Ref T and Alt C, which is a functional locus; SNP No. 291 at Genome position 10130687 of Chromosome D07 with Ref G and Alt C, which is a functional locus; SNP No. 292 at Genome position 54754308 of Chromosome D07 with Ref T and Alt C, which is a functional locus; SNP No. 293 at Genome position of Chromosome D07 with 54818140 Ref G and Alt A, which is a functional locus; SNP No. 294 at Genome position 54821539 of Chromosome DO7 with Ref T and Alt A, which is a functional locus; SNP No. 295 at Genome position 2748641 of Chromosome D08 with Ref C and Alt T, which is a functional locus; SNP No. 296 at Genome position 430878 of Chromosome D08 with Ref C and Alt A, which is a functional locus; SNP No. 297 at Genome position 4868113 of Chromosome D08 with Ref C and Alt T, which is a functional locus; SNP No. 298 at Genome position 608978 of Chromosome D08 with Ref C and Alt T, which is a functional locus; SNP No. 299 at Genome position 49092148 of Chromosome D09 with Ref A and Alt G, which is a functional locus; SNP No. 300 at Genome position 6069425 of Chromosome D09 with Ref C and Alt T, which is a functional locus; SNP No. 301 at Genome position 63443095 of Chromosome D10 with Ref G and Alt C, which is a functional locus; SNP No. 302 at Genome position 63918764 of Chromosome D10 with Ref C and Alt T, which is a functional locus; SNP No. 303 at Genome position 64184291 of Chromosome D10 with Ref A and Alt T, which is a functional locus; SNP No. 304 at Genome position 826050 of Chromosome D10 with Ref A and Alt G, which is a functional locus; SNP No. 305 at Genome position 10658879 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 306 at Genome position 13890576 of Chromosome D11 with Ref T and Alt C, which is a functional locus; SNP No. 307 at Genome position 21988164 of Chromosome D11 with Ref G and Alt A, which is a functional locus; SNP No. 308 at Genome position 23924389 of Chromosome D11 with Ref T and Alt A, which is a functional locus; SNP No. 309 at Genome position 23924409 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 310 at Genome position 23944152 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 311 at Genome position 24002127 of Chromosome D11 with Ref A and Alt G, which is a functional locus; SNP No. 312 at Genome position 24004889 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 313 at Genome position 24034422 of Chromosome D11 with Ref G and Alt C, which is a functional locus; SNP No. 314 at Genome position 24036519 of Chromosome D11 with Ref A and Alt G, which is a functional locus; SNP No. 315 at Genome position 3229941 of Chromosome D11 with Ref A and Alt G, which is a functional locus; SNP No. 316 at Genome position 3230659 of Chromosome D11 with Ref C and Alt T, which is a functional locus; SNP No. 317 at Genome position 62899476 of Chromosome D11 with Ref G and Alt A, which is a functional locus; SNP No. 318 at Genome position 1201966 of Chromosome D12 with Ref C and Alt A, which is a functional locus; SNP No. 319 at Genome position position 69399954 of Chromosome A01 with Ref A and Alt G, which is not a functional locus; SNP No. 361 at Genome position 72356977 of Chromosome A01 with Ref A and Alt G, which is not a functional locus; SNP No. 362 at Genome position 73066978 of Chromosome A01 with Ref C and Alt T, which is not a functional locus; SNP No. 363 at Genome position 83056539 of Chromosome A01 with Ref G and Alt C, which is not a functional locus; SNP No. 364 at Genome position 9307886 of Chromosome A01 with Ref T and Alt C, which is not a functional locus; SNP No. 365 at Genome position 94433050 of Chromosome A01 with Ref C and Alt G, which is not a functional locus; SNP No. 366 at Genome position 97114671 of Chromosome A01 with Ref G and Alt A, which is not a functional locus; SNP No. 367 at Genome position 9928461 of Chromosome A01 with Ref A and Alt G, which is not a functional locus; SNP No. 368 at Genome position 9992909 of Chromosome A01 with Ref T and Alt C, which is not a functional locus; SNP No. 369 at Genome position 104702522 of Chromosome A02 with Ref C and Alt G, which is not a functional locus; SNP No. 370 at Genome position 106564817 of Chromosome A02 with Ref G and Alt A, which is not a functional locus; SNP No. 371 at Genome position 126465 of Chromosome A02 with Ref A and Alt G, which is not a functional locus; SNP No. 372 at Genome position 18537125 of Chromosome A02 with Ref A and Alt G, which is not a functional locus; SNP No. 373 at Genome position 19418124 of Chromosome A02 with Ref T and Alt C, which is not a functional locus; SNP No. 374 at Genome position 3608260 of Chromosome A02 with Ref T and Alt A, which is not a functional locus; SNP No. 375 at Genome position 3753565 of Chromosome A02 with Ref C and Alt G, which is not a functional locus; SNP No. 376 at Genome position 39122586 of Chromosome A02 with Ref T and Alt C, which is not a functional locus; SNP No. 377 at Genome position 44761669 of Chromosome A02 with Ref C and Alt A, which is not a functional locus; SNP No. 378 at Genome position 44836527 of Chromosome A02 with Ref T and Alt C, which is not a functional locus; SNP No. 379 at Genome position 52198772 of Chromosome A02 with Ref A and Alt G, which is not a functional locus; SNP No. 380 at Genome position 63519897 of Chromosome A02 position 47819857 of Chromosome A07 with Ref A and Alt G, which is not a functional locus; SNP No. 502 at Genome position 54517045 of Chromosome A07 with Ref G and Alt A, which is not a functional locus; SNP No. 503 at Genome position 64238084 of Chromosome A07 with Ref C and Alt T, which is not a functional locus; SNP No. 504 at Genome position 80217572 of Chromosome A07 with Ref C and Alt T, which is not a functional locus; SNP No. 505 at Genome position 80388490 of Chromosome A07 with Ref A and Alt T, which is not a functional locus; SNP No. 506 at Genome position 82916184 of Chromosome A07 with Ref T and Alt C, which is not a functional locus; SNP No. 507 at Genome position 83105438 of Chromosome A07 with Ref G and Alt A, which is not a functional locus; SNP No. 508 at Genome position 8422752 of Chromosome A07 with Ref G and Alt C, which is not a functional locus; SNP No. 509 at Genome position 89161736 of Chromosome A07 with Ref A and Alt G, which is not a functional locus; SNP No. 510 at Genome position 9478030 of Chromosome A07 with Ref A and Alt C, which is not a functional locus; SNP No. 511 at Genome position 10429943 of Chromosome A08 with Ref C and Alt G, which is not a functional locus; SNP No. 512 at Genome position 106893339 of Chromosome A08 with Ref C and Alt T, which is not a functional locus; SNP No. 513 at Genome position 109696323 of Chromosome A08 with Ref G and Alt A, which is not a functional locus; SNP No. 514 at Genome position 112025642 of Chromosome A08 with Ref C and Alt T, which is not a functional locus; SNP No. 515 at Genome position 113464785 of Chromosome A08 with Ref A and Alt G, which is not a functional locus; SNP No. 516 at Genome position 116730869 of Chromosome A08 with Ref A and Alt G, which is not a functional locus; SNP No. 517 at Genome position 1713011 of Chromosome A08 with Ref T and Alt C, which is not a functional locus; SNP No. 518 at Genome position 19121855 of Chromosome A08 with Ref C and Alt A, which is not a functional locus; SNP No. 519 at Genome position 20388949 of Chromosome A08 with Ref G and Alt A, which is not a functional locus; SNP No. 520 at Genome position 25642734 of Chromosome A08 with Ref C and Alt T, which is not a functional locus; SNP No. 521 at Genome position 3134551 of Chromosome D13 with Ref A and Alt T, which is not a functional locus; SNP No. 885 at Genome position 39085839 of Chromosome D13 with Ref G and Alt A, which is not a functional locus; SNP No. 886 at Genome position 42257012 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 887 at Genome position 49441679 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 888 at Genome position 49745988 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 889 at Genome position 55874078 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 890 at Genome position 57525048 of Chromosome D13 with Ref T and Alt G, which is not a functional locus; SNP No. 891 at Genome position 58479149 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 892 at Genome position 63605335 of Chromosome D13 with Ref A and Alt G, which is not a functional locus; SNP No. 893 at Genome position 64125386 of Chromosome D13 with Ref T and Alt C, which is not a functional locus; and SNP No. 894 at Genome position 9724970 of Chromosome D13 with Ref T and Alt A, which is not a functional locus, wherein, Ref represents base type of a SNP locus in a reference genome, and Alt represents base type of SNP locus in a population.
2 . The liquid-phase chip for Gossypium hirsutum L. according to claim 1 , wherein the probes for detecting the 14 major transgenes consist of SEQ ID NOs. 1-14.