In vitro diagnosis method for periodontal diseases, and Pg bacterium detection method
The in vitro diagnostic method for periodontal disease of the present invention includes bringing a sample derived from an oral cavity of a subject into contact with an electrode to perform electrochemical measurement in the presence of a glucogenic amino acid and an electron mediator under an anaerobic environment and providing information for judging that periodontal disease is progressing in the oral cavity of the subject when current generation is detected as a result of the electrochemical measurement. According to the in vitro diagnostic method for periodontal disease of the present invention, it is possible to provide information for judging the progress of periodontal disease by simple operation.
1 . An in vitro diagnostic method for periodontal disease, comprising:
bringing a sample derived from an oral cavity of a subject into contact with an electrode to perform electrochemical measurement in the presence of a glucogenic amino acid and an electron mediator under an anaerobic environment; and
providing information for judging that periodontal disease is progressing in the oral cavity of the subject when a current generated by anaerobic metabolism of periodontopathogenic bacteria is detected as a result of the electrochemical measurement.
2 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the glucogenic amino acid includes at least one selected from the group consisting of arginine, histidine, aspartic acid, and glutamic acid.
3 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the electron mediator includes at least one selected from the group consisting of flavin mononucleotide, riboflavin, and 2-hydroxy-1,4-naphthoquinone.
4 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the electron mediator is 2-hydroxy-1,4-naphthoquinone.
5 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the method of the electrochemical measurement is a method by controlling a potential of the electrode and measuring a current flowing in the electrode as a function of time.
6 . The in vitro diagnostic method for periodontal disease according to claim 5 , wherein the potential is within a range of greater than 0 V with respect to a silver-silver chloride electrode and less than the upper limit of a potential window.
7 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the information for judging includes at least one measurement result selected from the group consisting of a time from start of the electrochemical measurement to detection of the current generation and a maximum value of current density of the generated current.
8 . The in vitro diagnostic method for periodontal disease according to claim 7 , wherein the information for judging includes comparison information between a predetermined reference value and the measurement result.
9 . The in vitro diagnostic method for periodontal disease according to claim 1 , wherein the periodontal disease comprises pathogenic bacteria, wherein the pathogenic bacteria of the periodontal disease includes Porphyromonas gingivalis.
10 . A Porphyromonas gingivalis (Pg) bacterium detection method comprising:
bringing a sample into contact with an electrode to perform electrochemical measurement in the presence of a glucogenic amino acid and 2-hydroxy-1,4-naphthoquinone under an anaerobic environment; and
providing information for judging that Pg is contained in the sample when current generation is detected as a result of the electrochemical measurement.
11 . The Pg bacterium detection method according to claim 10 , wherein a method of the electrochemical measurement is a method including the steps of controlling a potential of the electrode and measuring a current flowing in the electrode as a function of time.
12 . The Pg bacterium detection method according to claim 11 , wherein the potential is within a range of greater than 0 V with respect to a silver-silver chloride electrode and less than the upper limit of a potential window.
13 . The Pg bacterium detection method according to claim 10 , wherein the information for judging includes at least one measurement result selected from the group consisting of a time from start of the electrochemical measurement to detection of the current generation and a maximum value of current density of the generated current.
14 . The Pg bacterium detection method according to claim 13 , wherein the information for judging includes comparison information between a predetermined reference value and the measurement result.
15 . The in vitro diagnostic method for periodontal disease according to claim 3 , wherein the periodontal disease comprises pathogenic bacteria, wherein the pathogenic bacteria of the periodontal disease includes Pg.
16 . The in vitro diagnostic method for periodontal disease according to claim 4 , wherein the periodontal disease comprises pathogenic bacteria, wherein the pathogenic bacteria of the periodontal disease includes Pg.
17 . The in vitro diagnostic method for periodontal disease according to claim 3 , wherein a method of the electrochemical measurement is a method including the steps of controlling a potential of the electrode and measuring a current flowing in the electrode as a function of time.
18 . The in vitro diagnostic method for periodontal disease according to claim 17 , wherein the potential is within a range of greater than 0 V with respect to a silver-silver chloride electrode and less than the upper limit of a potential window.
19 . The in vitro diagnostic method for periodontal disease according to claim 18 , wherein the electron mediator is 2-hydroxy-1,4-naphthoquinone.
20 . The in vitro diagnostic method for periodontal disease according to claim 19 , wherein the periodontal disease comprises pathogenic bacteria, wherein the pathogenic bacteria of the periodontal disease includes Pg.