Molded flow channel
A nanopore based sequencing system includes a plurality of nanopore sensors. Each nanopore sensor has a portion for receiving a fluid. The nanopore based sequencing system includes a fluid chamber configured to guide the fluid over the plurality of nanopore sensors and an inlet configured to deliver the fluid into the fluid chamber. At least a portion of the fluid chamber is made of a material that has been molded around at least a portion of an electrode.
1 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the nanopore sensor array is fabricated on a sequencing chip, wherein the sequencing chip is electrically connected to a printed circuit board with a plurality of wire bonds, wherein the wire bonds are covered by an encapsulant configured to prevent the wire bonds from coming into contact with liquids;
a counter electrode;
a flow channel disposed over the nanopore sensor array;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is located proximate a second side of the nanopore sensor array, wherein the first side is opposite the second side.
2 . The nanopore based sequencing system of claim 1 , wherein the nanopore sensor array comprises one million nanopore sensors.
3 . The nanopore based sequencing system of claim 1 , wherein the cell is configured to support a membrane and a nanopore disposed in the membrane.
4 . The nanopore based sequencing system of claim 3 , wherein the nanopore is a protein nanopore.
5 . The nanopore based sequencing system of claim 4 , wherein the protein nanopore is attached to a polymerase.
6 . The nanopore based sequencing system of claim 5 , wherein the polymerase is associated with a nucleic acid molecule to be sequenced.
7 . The nanopore based sequencing system of claim 6 , wherein the nucleic acid molecule is circular.
8 . The nanopore based sequencing system of claim 1 , wherein the nanopore sensors are organized into a plurality of sensor banks.
9 . The nanopore based sequencing system of claim 1 , further comprising a fan-out plenum in fluid communication with the inlet, the fan-out plenum configured to receive fluid from the inlet and dispense the fluid outwardly into the flow channel.
10 . The nanopore based sequencing system of claim 9 , further comprising a reverse fan-out plenum in fluid communication with the outlet, the reverse fan-out plenum configured to receive fluid from the fluid channel and direct the fluid to the outlet.
11 . The nanopore based sequencing system of claim 1 , wherein the encapsulant is an adhesive.
12 . The nanopore based sequencing system of claim 1 , wherein the adhesive is configured to seal the flow channel over the sensor array and to the printed circuit board.
13 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the cell is configured to support a membrane and a nanopore disposed in the membrane, wherein the nanopore is attached to a polymerase, wherein the polymerase is associated with a nucleic acid molecule to be sequenced, wherein the nucleic acid molecule is circular, wherein the nanopore sensor array is fabricated on a sequencing chip, wherein the sequencing chip is electrically connected to a printed circuit board with a plurality of wire bonds, wherein the wire bonds are covered by an encapsulant configured to prevent the wire bonds from coming into contact with liquids;
a counter electrode;
a flow channel disposed over the nanopore sensor array;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is located proximate a second side of the nanopore sensor array, wherein the first side is opposite the second side.
14 . The nanopore based sequencing system of claim 13 , wherein the nanopore sensor array comprises one million nanopore sensors.
15 . The nanopore based sequencing system of claim 13 , wherein the nanopore sensors are organized into a plurality of sensor banks.
16 . The nanopore based sequencing system of claim 13 , further comprising a fan-out plenum in fluid communication with the inlet, the fan-out plenum configured to receive fluid from the inlet and dispense the fluid outwardly into the flow channel.
17 . The nanopore based sequencing system of claim 16 , further comprising a reverse fan-out plenum in fluid communication with the outlet, the reverse fan-out plenum configured to receive fluid from the fluid channel and direct the fluid to the outlet.
18 . The nanopore based sequencing system of claim 14 , wherein the encapsulant is an adhesive that is also configured to seal the flow channel over the sensor array and to the printed circuit board.