Needle drive, system and method

1 . A liquid chromatography system comprising:

a solvent delivery system;

a sample manager having a thermal chamber, the thermal chamber including:

a sampling mechanism mounted within the thermal chamber, the sampling mechanism including;

a sample platter mounted therein, the sample platter configured to rotate about a first vertical axis, the sample platter including at least one carrier bay for receiving a sample vial carrier having an array of sample vials loaded therein;

a needle arm configured to rotate about a second vertical axis that is in a fixed position within the thermal chamber, wherein a combination of the rotation of the needle arm and the rotation of the sample platter provides complete coverage over an entirety of a working portion of the sample platter; and

a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system including a sample needle attached to the needle arm, the sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream;

a liquid chromatography column located downstream from the solvent delivery system and the sample delivery system; and

a detector located downstream from the liquid chromatography column.

2 . The liquid chromatography system of claim 1 , wherein the needle arm includes a belt and pulley drive mechanism.

3 . The liquid chromatography system of claim 1 , wherein the needle arm includes a magnetic encoder configured to determine rotational position of the needle arm.

4 . The liquid chromatography system of claim 1 , wherein the sample delivery system includes a fluidic tube located between the sample needle and the liquid chromatography column, wherein the fluidic tube includes a coiled portion configured to expand and contract during rotation of the needle arm.

5 . The liquid chromatography system of claim 1 , wherein the sample platter is circular and wherein the at least one carrier bay includes a first bay, a second bay, a third bay and a fourth bay disposed equidistant about a perimeter of the circular sample platter.

6 . The liquid chromatography system of claim 1 , further comprising a control system, the control system configured to control the rotational movement of each of the sample platter and the needle arm, the control system configured to control a calibration process that includes the steps of:

moving the sample platter so that a first opening in the sample platter aligns with the sample needle on the needle arm;

moving the needle arm above the aligned first opening and recording a first encoder position of each of the sample platter and the needle arm;

moving the sample platter so that a second opening in the sample platter aligns with the sample needle on the needle arm;

moving the needle arm above the aligned second opening and recording a second encoder position of each of the sample platter and the needle arm; and

with the known first and second encoder positions, back-calculating the geometric parameters of the sample platter and the needle arm to calibrate the movement of the sample platter and the needle arm.

7 . A liquid chromatography sample manager comprising:

a thermal chamber;

a sample platter mounted in the thermal chamber, the sample platter configured to rotate about a first vertical axis, the sample platter including at least one carrier bay for receiving a sample vial carrier having an array of sample vials loaded therein;

a needle arm mounted within the thermal chamber, the needle arm configured to rotate about a second vertical axis that is in a fixed position within the thermal chamber, wherein a combination of the rotation of the needle arm and the rotation of the sample platter provides complete coverage over an entirety of a working portion of the sample platter; and

a sample delivery system in fluidic communication with a solvent delivery system, the sample delivery system including a sample needle attached to the needle arm, the sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream.

8 . The liquid chromatography sample manager of claim 7 , wherein the needle arm includes a belt and pulley drive mechanism.

9 . The liquid chromatography sample manager of claim 7 , wherein the needle arm includes a magnetic encoder configured to determine rotational position of the needle arm.

10 . The liquid chromatography sample manager of claim 7 , wherein the sample delivery system includes a fluidic tube located between the sample needle and a liquid chromatography column, wherein the fluidic tube includes a coiled portion configured to expand and contract during rotation of the needle arm.

11 . The liquid chromatography sample manager of claim 7 , wherein the sample platter is circular and wherein the at least one carrier bay includes a first bay, a second bay, a third bay and a fourth bay disposed equidistant about a perimeter of the circular sample platter.

12 . The liquid chromatography sample manager of claim 7 , further comprising a control system, the control system configured to control the rotational movement of each of the sample platter and the needle arm, the control system configured to control a calibration process that includes the steps of:

moving the sample platter so that a first opening in the sample platter aligns with the sample needle on the needle arm;

moving the needle arm above the aligned first opening and recording a first encoder position of each of the sample platter and the needle arm;

moving the sample platter so that a second opening in the sample platter aligns with the sample needle on the needle arm;

moving the needle arm above the aligned second opening and recording a second encoder position of each of the sample platter and the needle arm; and

with the known first and second encoder positions, back-calculating the geometric parameters of the sample platter and the needle arm to calibrate the movement of the sample platter and the needle arm.

13 . The liquid chromatography sample manager of claim 7 , wherein the needle arm is configured to rotate about the second vertical axis at least 45 degrees and wherein the sample platter is configured to rotate about the first vertical axis 360 degrees.

14 . A needle drive for a liquid chromatography system comprising:

a base including a shaft configured to rotate about a vertical axis, the base attachable to an interior of a sample manager of a liquid chromatography system such that the vertical axis remains in a fixed position; and

a needle assembly attached to and extending from the base such that rotation of the shaft about the vertical axis rotates the needle assembly about the vertical axis, the needle assembly including a sample needle and a drive system including a sample needle motor configured to impart vertical movement of the sample needle, wherein the needle assembly is attachably removable from the base with a plurality of accessible coupling devices, wherein the sample needle motor is configured to rotate about the vertical axis with the needle assembly.

15 . The needle drive of claim 14 , wherein the needle assembly further includes a puncture needle, and wherein the needle assembly further includes a puncture needle motor configured to impart vertical movement on the puncture needle independently from the vertical movement of the sample needle, wherein the puncture needle motor is configured to rotate about the vertical axis with the needle assembly.

16 . The needle drive of claim 14 , wherein the needle assembly further includes a stripper foot movable in a vertical direction, wherein the stripper foot includes an opening through which the puncture needle is configured to extend during puncturing.

17 . The needle drive of claim 16 , further comprising a sensor system, the sensor system including a flexible circuit board attached to the base and configured to bend with the rotation of the shaft about the vertical axis.

18 . The needle drive of claim 17 , the sensor system further comprising a stripper foot movement sensor configured to determine that the stripper foot has been moved in a vertical direction a predetermined distance, a sample needle movement sensor configured to determine that the sample needle has been moved in a vertical direction to a sample needle home position, and a puncture needle movement sensor configured to determine that the puncture needle has been moved in a vertical direction to a puncture needle home position.

19 . The needle drive of claim 14 , further comprising a shaft motor configured to impart rotation on the shaft about the vertical axis, and further comprising a magnetic encoder configured to maintain precise rotational position of the shaft of the base.

20 . A liquid chromatography system comprising:

a solvent delivery system;

a sample manager having a thermal chamber, the thermal chamber including:

a sampling mechanism mounted within the thermal chamber, the sampling mechanism including;

a sample platter mounted therein, the sample platter configured to rotate about a first vertical axis;

a needle arm configured to rotate about a second vertical axis that is in a fixed position within the thermal chamber, wherein a combination of the rotation of the needle arm and the rotation of the sample platter provides complete coverage over an entirety of a working portion of the sample platter; and

a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system including a sample needle attached to the needle arm, the sample delivery system configured to transfer a first sample from a first sample vial carrier located in the sample platter into a chromatographic flow stream;

a liquid chromatography column located downstream from the solvent delivery system and the sample delivery system; and

a detector located downstream from the liquid chromatography column.