Biopesticide formulation including

1 . A biopesticide formulation comprising:

a dried extract of Plicosepalus acaciae;

a dried extract of Ocimum basilicum ; and

a dried extract of Eucalyptus tereticornis,

wherein the formulation comprises a 3:4:4 ratio of the Plicosepalus acaciae dried extract, the Ocimum basilicum dried extract, and the Eucalyptus tereticornis dried extract.

2 . The biopesticide formulation of claim 1 , further comprising glycerol and polysorbate 80.

3 . The biopesticide formulation of claim 2 , wherein the biopesticide formulation is formulated as a nano-emulsion.

4 . The biopesticide formulation of claim 1 , wherein the biopesticide has an LD 50 of 4.57 μg/larva and a LC 50 of 247 μg/mL against Rhynchophorus ferrugineus.

5 . A method of making the biopesticide formulation of claim 1 , the method comprising:

providing Plicosepalus acaciae leaves;

drying the Plicosepalus acaciae leaves to obtain dried Plicosepalus acaciae leaves;

grinding the dried Plicosepalus acaciae leaves to obtain Plicosepalus acaciae powder;

extracting the Plicosepalus acaciae powder using methanol to obtain a first Plicosepalus acaciae extract;

concentrating first Plicosepalus acaciae extract to obtain a second Plicosepalus acaciae extract;

providing Eucalyptus tereticornis seeds;

delinting, sterilizing, and germinating the Eucalyptus tereticornis seeds;

cutting leaf segments from the germinated Eucalyptus tereticornis;

growing the Eucalyptus tereticornis leaf segments with a growth medium to obtain Eucalyptus tereticornis callus;

extracting the Eucalyptus tereticornis callus with methanol to obtain an Eucalyptus tereticornis extract;

providing Ocimum basilicum seeds;

delinting, sterilizing, and germinating the Ocimum basilicum seeds;

cutting leaf segments from the germinated Ocimum basilicum;

growing the Ocimum basilicum leaf segments with a growth medium to obtain Ocimum basilicum callus;

extracting the Ocimum basilicum callus with methanol to obtain an O. basilicum extract;

drying the second Plicosepalus acaciae extract, the Eucalyptus tereticornis extract, and the Ocimum basilicum extract; and

mixing the dried Plicosepalus acaciae extract, the dried Eucalyptus tereticornis extract, and the dried Ocimum basilicum extract to obtain the biopesticide formulation.

6 . The method of claim 5 , wherein the Plicosepalus acaciae leaves are dried by a method selected from the group consisting of sun drying, oven drying, and air drying.

7 . The method of claim 5 , wherein grinding the Plicosepalus acaciae leaves comprises grinding the Plicosepalus acaciae leaves using a mortar and pestle.

8 . The method of claim 5 , wherein concentrating first Plicosepalus acaciae extract comprises concentrating the first Plicosepalus acaciae extract at about 30° C. to about 45° C. using a rotary evaporator.

9 . The method of claim 5 , wherein growing the Eucalyptus tereticornis leaf segments with a growth medium comprises growing the Eucalyptus tereticornis leaf segments on Murashige and Skoog medium.

10 . The method of claim 9 , wherein the Murashige and Skoog medium comprises about 3% sucrose and about 6.0 g/L of agar.

11 . The method of claim 9 , wherein the Murashige and Skoog medium comprises about 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D), about 0.5 mg/L kinetin, about 0.5 mg/L Naphthaleneacetic Acid, and about 1 mg/L Indole-3-Butyric Acid.

12 . The method of claim 9 , wherein the Murashige and Skoog medium comprises about 100 mM of methyl jasmonate, about 0.3 mg/L kinetin, about 0.5 mg/L 2,4-D, about 0.2 mg/L Naphthaleneacetic Acid, and about 1 mg/L Indole-3-Butyric Acid, and Verticillium dahliae.

13 . The method of claim 5 , wherein growing the Ocimum basilicum leaf segments with a growth medium comprises growing the Ocimum basilicum leaf segments on Murashige and Skoog medium.

14 . The method of claim 13 , wherein the Murashige and Skoog medium comprises about 3% sucrose and about 6.0 g/L of agar.

15 . The method of claim 13 , wherein the Murashige and Skoog medium comprises about 0.5 mg/L 2,4-D, about 0.5 mg/L kinetin, about 0.5 mg/L Naphthaleneacetic Acid, and about 1 mg/L Indole-3-Butyric Acid.

16 . The method of claim 13 , wherein the Murashige and Skoog medium comprises about 100 mM of methyl jasmonate, about 0.3 mg/L kinetin, about 0.5 mg/L 2,4-D, about 0.2 mg/L Naphthaleneacetic Acid, and about 1 mg/L Indole-3-Butyric Acid, and Verticillium dahliae.

17 . The method of claim 5 , wherein growing the Ocimum basilicum leaf segments with a growth medium comprises growing the Ocimum basilicum leaf segments on Linsmaier and Skoog medium, and wherein growing the Eucalyptus tereticornis leaf segments with a growth medium comprises growing the Eucalyptus tereticornis leaf segments on Linsmaier and Skoog medium.

18 . The method of claim 5 , comprising:

mixing the biopesticide formulation is with a surfactant and a co-surfactant to obtain a first mixture;

vortexing the first mixture;

adding deionized water to the first mixture to obtain a second mixture;

and sonicating the second mixture to obtain a nano-emulsion comprising the biopesticide formulation.

19 . A method of inhibiting or killing Rhynchophorus ferrugineus , comprising administering the biopesticide formulation of claim 1 to a plant in need thereof.