Optimized synthetic consensus immunogenic compositions targeting fibroblast activation protein
Provided herein is an immunogenic composition comprising a synthetic consensus FAP antigen. Also disclosed herein is a method of treating or preventing a tumor associated pathology in a subject in need thereof, by administering the immunogenic composition to the subject.
1 . An isolated nucleic acid molecule encoding a single polypeptide, wherein the single polypeptide comprises the amino acid sequence of SEQ ID NO:4.
2 . The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is selected from the group consisting of a DNA molecule and an RNA molecule.
3 . The nucleic acid molecule of claim 1 , wherein the nucleic acid encoding the single polypeptide comprises a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 4 comprises a nucleotide sequence comprising SEQ ID NO: 3.
4 . The nucleic acid molecule of claim 1 , wherein the encoded polypeptide is operably linked to at least one regulatory sequence selected from the group consisting of a start codon, an IgE leader sequence and a stop codon.
5 . An isolated expression vector comprising the nucleic acid molecule of claim 1 .
6 . An isolated viral particle comprising the nucleic acid molecule of claim 1 .
7 . An isolated peptide consisting of the amino acid sequence of SEQ ID NO:4.
8 . An immunogenic composition consisting of a peptide of claim 7 and a pharmaceutically acceptable excipient.
9 . An immunogenic composition comprising a nucleic acid molecule of claim 1 .