Adipose compositions and methods of use thereof
Disclosed are devitalized adipose tissue. Disclosed are compositions comprising devitalized adipose tissue. In some aspects, the compositions further comprise a cryopreservation or lyophilization solution. Disclosed are cryopreserved or lyophilized devitalized adipose tissue. Disclosed are methods of augmenting a soft tissue site of a subject in need thereof comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having fat pad atrophy comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having lipodystrophy comprising administering to the subject a composition comprising devitalized adipose tissue. Disclosed are methods of treating a subject having a metabolic disease or condition comprising administering to the subject a composition comprising devitalized adipose tissue.
1 . A cryopreserved composition comprising autologous or allogeneic devitalized adipose tissue,
wherein the devitalized adipose tissue comprises at least 50% of native lipids,
wherein the devitalized adipose tissue comprises at least 70% of native growth factors,
wherein the devitalized adipose tissue is not decellularized,
wherein the devitalized adipose tissue retains its native structure,
wherein the composition does not comprise free lipids,
wherein the composition does not comprise TNFα, and
wherein the devitalized adipose tissue comprises less than 5% native viable cells.
2 . The composition of claim 1 , wherein the devitalized adipose tissue is minced.
3 . The composition of claim 2 , wherein the minced devitalized adipose tissue comprises pieces of adipose tissue less than 1 mm in size.
4 . The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.
5 . The composition of claim 1 , further comprising one or more lyopreservative agents.
6 . The composition of claim 5 , wherein the one or more lyopreservative agents is a sugar.
7 . The composition of claim 6 , wherein the sugar is trehalose.
8 . The composition of claim 5 , wherein the one or more lyopreservative agents is human serum albumin.
9 . The composition of claim 1 , wherein the devitalized adipose tissue comprises less than 5%, 2%, 1%, 0.5%, 0.2%, 0.1%, 0.05% or 0.01% peroxidized lipids of total lipids.
10 . The composition of claim 1 , wherein the growth factors are angiogenic growth factors, vascular endothelial growth factors, insulin like growth factors, hepatocyte growth factor, platelet-derived growth factor, or fibroblast growth factors.
11 . The composition of claim 1 , wherein the devitalized adipose tissue is non-radiated.