Mixing disk assembly for use on centrifugal platform
A dynamic mixing disk assembly for a centrifugal platform has an inner disk and an outer disk. The inner disk comprises a first ring part and a second ring part configured to an outer periphery circumference of the first ring part. The first ring part comprises multiple or at least two pawls. The outer disk is rotatably disposed on the second ring part of the inner disk. The outer disk comprises multiple tapered recesses or at least two tapered recesses. When the pawls are engaged with the tapered recesses of the outer disk, the inner disk and the outer disk are able to rotate synchronously with the centrifugal platform. When the inner disk is stopped by the centrifugal platform, the outer disk will keep rotating, and the pawls disengage from the tapered recesses and move to the next tapered recesses.
1 . A mixing disk assembly for use on a centrifugal platform, comprising:
an inner separation disk with a first ring part and a second ring part disposed on the outer periphery of the first ring part, with the first ring part having multiple pawls;
the first ring part of the inner separation disk is provided with at least two eluent reservoirs, at least two recycling chambers, and at least one mixing chamber, wherein the at least two eluent reservoirs are in fluid communication with the at least two recycling chambers and the at least one mixing chamber through at least two metering units, and the at least one mixing chamber is in fluid communication with a siphoning channel and a separation column; and
an outer collection disk rotatably mounted on the second ring part of the inner separation disk, the outer collection disk having multiple tapered recesses that when the multiple pawls of the first ring part engage some of the multiple tapered recesses, the outer collection disk rotates synchronously with the inner separation disk, wherein each fraction collector comprises a feeding channel and multiple collecting chambers; and the outer collection disk has multiple fraction collectors in fluid communication with the separation column through a collection tube; and
when a rotational speed difference between the inner separation disk and the outer collection disk occurs, causing the outer collection disk to rotate relative to the inner separation disk and allowing the pawl to exit one tapered recess and switch to another tapered recess.
2 . The mixing disk assembly according to claim 1 , wherein the multiple pawls of the inner separation disk are each resiliently mounted on the first ring part by a spring.
3 . The mixing disk assembly according to claim 1 , wherein a first metering unit on a left side comprises an inverted Y-shaped structure designating a first metering channel firstly extended from a first eluent reservoir; further dividing as the inverted Y-shaped structure are a second metering channel toward to the recycling chamber and a third metering channel toward the mixing chamber; and a second metering unit on a right side comprises the inverted Y-shaped structure designating a first metering channel firstly extended from a second eluent reservoir and further dividing as the inverted Y-shaped structure are a second metering channel toward to the mixing chamber and a third metering channel toward the recycling chamber.
4 . The mixing disk assembly according to claim 1 , wherein during a loading phase of the centrifugal platform, at least two corresponding eluents are loaded into the respective at least two eluent reservoirs.
5 . The mixing disk assembly according to claim 2 , wherein during a loading phase of the centrifugal platform, at least two corresponding eluents are loaded into the respective at least two eluent reservoirs.
6 . The mixing disk assembly according to claim 3 , wherein during a loading phase of the centrifugal platform, at least two corresponding eluents are loaded into the respective at least two eluent reservoirs.
7 . The mixing disk assembly according to claim 4 , wherein during a metering phase, the centrifugal platform rotates at a specific rotational speed to allow the at least two eluents in the at least two eluent reservoirs to flow through the metering unit into the at least one mixing chamber and recycling chambers.
8 . The mixing disk assembly according to claim 7 , wherein by varying the rotational speed of the centrifugal platform, a first eluent and a second eluent flow through a second metering channel and third metering channel in a specific ratio into a first recycling chamber, a second recycling chamber, and the mixing chamber.
9 . The mixing disk assembly according to claim 8 , wherein during a mixing phase, the centrifugal platform employs an acceleration and deceleration mode to mix the at least two eluents in the at least one mixing chamber to obtain a mixed eluent.
10 . The mixing disk assembly according to claim 9 , wherein during a separation phase, the centrifugal platform employs a high-speed rotation mode to elute a sample from the separation column by the mixed eluent.
11 . The mixing disk assembly according to claim 10 , wherein during a collection phase, the centrifugal platform collects an eluate flowing out from an outlet of the separation column by multiple collecting chambers of the multiple fraction collectors through the feeding channel.
12 . The mixing disk assembly according to claim 11 , wherein during a switching phase, the centrifugal platform induces the rotational speed difference between the inner separation disk and the outer collection disk by stopping or reducing the rotational speed, thereby causing the outer collection disk to rotate relative to the inner separation disk.