Compositions, systems, and methods of making and using encapsulated lyophilised microspheres
The present disclosure relates to a composition comprising a shell surrounding a core, wherein the core comprises one or more lyophilised microspheres. Also described herein is a method comprising providing one or more lyophilised microspheres; and coating the one or more lyophilised microspheres with a shell under conditions effective to encapsulate the one or more lyophilised microspheres. The present disclosure further relates to a system comprising one or more composition as described herein, and one or more lyophilised cake, wherein the one or more composition and the one or more lyophilised cake are combined under conditions effective to form a rehydration system. Also described herein is a method of controlling release of one or more encapsulated microspheres comprising providing a composition as described herein and mixing the composition with a rehydration solution under a first condition effective to control release of one or more lyophilised microspheres from the composition.
1 . A composition comprising:
a shell surrounding a core, wherein said shell comprises a shell additive and said shell additive comprises a static mitigation material, a moisture barrier material, or a combination thereof, and wherein said core comprises one or more lyophilised microspheres;
wherein said core comprises a polymerase.
2 . The composition of claim 1 , wherein the shell additive is a static mitigation material present in an amount no more than 40% w/w concentration of the shell.
3 . The composition of claim 1 , wherein the shell additive is a moisture barrier material present in an amount no more than 90% w/w concentration of the shell.
4 . The composition of claim 1 , wherein the shell additive comprises one or more of a polymer, a copolymer, a block copolymer, a second polyvinyl alcohol (PVA), an ammonium salt, a stearate derivative, an oleate derivative, a laurate derivative, a polyether compound, an amino acid, tocopherol acetate, piperidyl sebacate, sodium salt, a buffer, a chelating agent, imidazolium salt, polyaniline, or any combination thereof.
5 . The composition of claim 4 , wherein the polyether compound is selected from polyethylene glycol, polypropylene glycol, a block copolymer derived from ethylene oxide (EO) and propylene oxide (PO), or any combination thereof.
6 . The composition of claim 4 , wherein the amino acid is selected from one or more of leucine, isoleucine, phenylalanine, or any combination thereof.
7 . The composition of claim 4 , wherein the sodium salt is selected from one or more of sodium chloride, sodium bisulfite, sodium citrate, or any combination thereof.
8 . The composition of claim 4 , wherein the buffer is Tris HCl.
9 . The composition of claim 4 , wherein the ammonium salt is selected from tetraalkyl ammonium chloride, tris(hydroxyethyl) alkylammonium chloride, or a combination thereof.
10 . The composition of claim 4 , wherein the imidazolium salt is selected from 1-ethyl-3-methyl-imidazolium salt, or polyquaternium, or copolymer of vinyl pyrrolidone and quaternized vinylimidazole, or a combination thereof.
11 . The composition of claim 1 , wherein the shell additive comprises ammonium salt, copolymer, polyvinyl alcohol graft polyethylene glycol copolymer, polyvinyl alcohol (PVA), or any combination thereof.
12 . The composition of claim 1 , wherein said core comprises one or more reagent selected from one or more enzyme, salt, surfactant, buffering agent, enzyme inhibitor, primer, nucleotide, organic osmolite, magnetic bead, molecular probe, crowding agent, labelled-nucleotide, a fluorophore, or any combination thereof.
13 . The composition of claim 1 , wherein said core further comprises one or more additional agent, wherein said one or more additional agent is selected from one or more sugar, one or more amino acid, one or more polymer, one or more mesoporous silica, one or more quaternary amine, and any combination thereof.
14 . The composition of claim 13 , wherein, when said one or more additional agent comprises one or more sugar, said one or more sugar is selected from trehalose, mannitol, cyclodextrin, dextran, sucrose, or any combination thereof.
15 . The composition of claim 13 , wherein, when said one or more additional agent comprises one or more amino acid, said one or more amino acid has a hydrophobic side chain.
16 . A composition comprising:
a shell surrounding a core, wherein said core comprises a core additive and said core additive comprises a static mitigation material;
wherein said core comprises a polymerase; and wherein
(i) the core additive is a static mitigation material present in an amount no more than 25% w/w concentration of the core, or
(ii) the core additive is in an amount between about 2% w/w and about 10% w/w concentration of the core.
17 . The composition of claim 16 , wherein the core additive comprises one or more of a polymer, a copolymer, a block copolymer, a second polyvinyl alcohol (PVA), an ammonium salt, an imidazolium salt, a polyether compound, or any combination thereof.
18 . The composition of claim 16 , wherein the core additive is a static mitigation material present in an amount no more than 25% w/w concentration of the core.
19 . The composition of claim 17 , wherein one or more of:
(i) the polyether compound is selected from polyethylene glycol, polypropylene glycol, a block copolymer derived from ethylene oxide (EO) and propylene oxide (PO), or any combination thereof;
(ii) the polymer is neutral, cationic, or anionic;
(iii) the ammonium salt is selected from tetraalkyl ammonium chloride, tris(hydroxyethyl) alkylammonium chloride, or a combination thereof; and
(iv) the imidazolium salt is selected from 1-ethyl-3-methyl-imidazolium salt, or polyquaternium, or copolymer of vinyl pyrrolidone and quaternized vinylimidazole, or a combination thereof.
20 . The composition of claim 16 , wherein said shell comprises more than one lyophilised microsphere, and wherein one of said reagents in a first of said more than one lyophilised microsphere is different from another of said reagents in a second of said more than one lyophilised microsphere.